SrasV1, PascalFrancis, Curation, bibRecord, 000488

Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus

Identifieur interne : 000488 ( PascalFrancis/Curation ); précédent : 000487; suivant : 000489

Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus

Auteurs : Masanobu Yamate [Japon] ; Makiko Yamashita [Japon] ; Toshiyuki Goto [Japon] ; Shoutaro Tsuji [Japon] ; Yong-Gang Li [Japon] ; Jiranan Warachit [Japon] ; Mikihiro Yunoki [Japon] ; Kazuyoshi Ikuta [Japon]

Source :

Microbes and infection [ 1286-4579 ] ; 2005.

RBID : Pascal:06-0256876

Descripteurs français

Pascal (Inist)
- Coronavirus, Cellule infectée, Nucléocapside, Protéine, Infection persistante, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Infected cell, Nucleocapsid, Persistent infection, Protein, Severe acute respiratory syndrome.

Abstract

Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.

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A08	`01`	`1`	`ENG`	`@1 Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus`
A11	`01`	`1`		`@1 YAMATE (Masanobu)`
A11	`02`	`1`		`@1 YAMASHITA (Makiko)`
A11	`03`	`1`		`@1 GOTO (Toshiyuki)`
A11	`04`	`1`		`@1 TSUJI (Shoutaro)`
A11	`05`	`1`		`@1 LI (Yong-Gang)`
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A14	`01`			`@1 Department of Virology, Research Institute for Microbial Diseases, Osaka University @2 Suita, Osaka 565-0871 @3 JPN @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut. @Z 8 aut.`
A14	`02`			`@1 School of Health Science, Faculty of Medicine, Kyoto University @2 Sakyo-ku, Kyoto 606-8507 @3 JPN @Z 3 aut.`
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C01	`01`		`ENG`	@0 Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.
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Pascal:06-0256876

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus</title>
<author><name sortKey="Yamate, Masanobu" sort="Yamate, Masanobu" uniqKey="Yamate M" first="Masanobu" last="Yamate">Masanobu Yamate</name>
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<author><name sortKey="Yamashita, Makiko" sort="Yamashita, Makiko" uniqKey="Yamashita M" first="Makiko" last="Yamashita">Makiko Yamashita</name>
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<author><name sortKey="Goto, Toshiyuki" sort="Goto, Toshiyuki" uniqKey="Goto T" first="Toshiyuki" last="Goto">Toshiyuki Goto</name>
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<author><name sortKey="Tsuji, Shoutaro" sort="Tsuji, Shoutaro" uniqKey="Tsuji S" first="Shoutaro" last="Tsuji">Shoutaro Tsuji</name>
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<author><name sortKey="Warachit, Jiranan" sort="Warachit, Jiranan" uniqKey="Warachit J" first="Jiranan" last="Warachit">Jiranan Warachit</name>
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<author><name sortKey="Yunoki, Mikihiro" sort="Yunoki, Mikihiro" uniqKey="Yunoki M" first="Mikihiro" last="Yunoki">Mikihiro Yunoki</name>
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<author><name sortKey="Ikuta, Kazuyoshi" sort="Ikuta, Kazuyoshi" uniqKey="Ikuta K" first="Kazuyoshi" last="Ikuta">Kazuyoshi Ikuta</name>
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<series><title level="j" type="main">Microbes and infection</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
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<term>Severe acute respiratory syndrome</term>
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<term>Cellule infectée</term>
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<front><div type="abstract" xml:lang="en">Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.</div>
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<fA06><s2>15</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>YAMATE (Masanobu)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>YAMASHITA (Makiko)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>GOTO (Toshiyuki)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>TSUJI (Shoutaro)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>LI (Yong-Gang)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>WARACHIT (Jiranan)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>YUNOKI (Mikihiro)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>IKUTA (Kazuyoshi)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Virology, Research Institute for Microbial Diseases, Osaka University</s1>
<s2>Suita, Osaka 565-0871</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>School of Health Science, Faculty of Medicine, Kyoto University</s1>
<s2>Sakyo-ku, Kyoto 606-8507</s2>
<s3>JPN</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Research and Development Division, Benesis Corporation, 2-25-1, Shodai-Ohtani</s1>
<s2>Hirakata, Osaka 573-1153</s2>
<s3>JPN</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>1530-1540</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>26816</s2>
<s5>354000132876030090</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2006 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>59 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>06-0256876</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Microbes and infection</s0>
</fA64>
<fA66 i1="01"><s0>FRA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Cellule infectée</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Infected cell</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Célula infectada</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Nucléocapside</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Nucleocápside</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Protéine</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Protein</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Proteína</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Infection persistante</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Persistent infection</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Infección persistente</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>163</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus

Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus

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