SrasV1, PascalFrancis, Curation, bibRecord, 000201

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

Identifieur interne : 000201 ( PascalFrancis/Curation ); précédent : 000200; suivant : 000202

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

Auteurs : Jyh-Lyh Juang [Taïwan] ; Tsan-Chi Chen [Taïwan] ; SHIH SHENG JIANG [Taïwan] ; Chao A. Hsiung [Taïwan] ; Wei-Chen Chen [Taïwan] ; Guang-Wu Chen [Taïwan] ; Shiang-Ming Lin [Taïwan] ; Jih-Hui Lin [Taïwan] ; Shu-Chun Chiu [Taïwan] ; Yiu-Kay Lai [Taïwan]

Source :

Laboratory investigation [ 0023-6837 ] ; 2004.

RBID : Pascal:04-0548676

Descripteurs français

Pascal (Inist)
- Couplage, Réaction chaîne polymérase multiplex, Expression génique, RNA-directed DNA polymerase, Réaction chaîne polymérase RT, Gène, Génétique, Puce électronique, Analyse biochimique, Dosage, Syndrome respiratoire aigu sévère, Coronavirus, Biologie clinique, Biotechnologie.
Wicri :
- topic : Génétique, Biotechnologie.

English descriptors

KwdEn :
- Assay, Biochemical analysis, Biotechnology, Chip, Clinical biology, Coronavirus, Coupling, Gene, Gene expression, Genetics, Multiplex polymerase chain reaction, RNA-directed DNA polymerase, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome.

Abstract

An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Assay</term>
<term>Biochemical analysis</term>
<term>Biotechnology</term>
<term>Chip</term>
<term>Clinical biology</term>
<term>Coronavirus</term>
<term>Coupling</term>
<term>Gene</term>
<term>Gene expression</term>
<term>Genetics</term>
<term>Multiplex polymerase chain reaction</term>
<term>RNA-directed DNA polymerase</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>Severe acute respiratory syndrome</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Couplage</term>
<term>Réaction chaîne polymérase multiplex</term>
<term>Expression génique</term>
<term>RNA-directed DNA polymerase</term>
<term>Réaction chaîne polymérase RT</term>
<term>Gène</term>
<term>Génétique</term>
<term>Puce électronique</term>
<term>Analyse biochimique</term>
<term>Dosage</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Coronavirus</term>
<term>Biologie clinique</term>
<term>Biotechnologie</term>
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<front><div type="abstract" xml:lang="en">An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0023-6837</s0>
</fA01>
<fA02 i1="01"><s0>LAINAW</s0>
</fA02>
<fA03 i2="1"><s0>Lab. invest.</s0>
</fA03>
<fA05><s2>84</s2>
</fA05>
<fA06><s2>9</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>JUANG (Jyh-Lyh)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>CHEN (Tsan-Chi)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>SHIH SHENG JIANG</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>HSIUNG (Chao A.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>CHEN (Wei-Chen)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>CHEN (Guang-Wu)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>LIN (Shiang-Ming)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>LIN (Jih-Hui)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>CHIU (Shu-Chun)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>LAI (Yiu-Kay)</s1>
</fA11>
<fA14 i1="01"><s1>Division of Molecular and Genomic Medicine, National Health Research Institutes</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>President's Laboratory, National Health Research Institutes</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Biostatistics and Bioinformatics, National Health Research Institutes</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Center for Disease Control, Department of Health</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Life Science and Institute of Biotechnology, National Tsing Hua University</s1>
<s2>Hsinchu</s2>
<s3>TWN</s3>
<sZ>10 aut.</sZ>
</fA14>
<fA20><s1>1085-1091</s1>
</fA20>
<fA21><s1>2004</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>8078</s2>
<s5>354000110574040010</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>17 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>04-0548676</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Laboratory investigation</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B24</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A31</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Couplage</s0>
<s5>02</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coupling</s0>
<s5>02</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Acoplamiento</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Réaction chaîne polymérase multiplex</s0>
<s5>03</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Multiplex polymerase chain reaction</s0>
<s5>03</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Reacción cadena polimerasa multiplex</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Expression génique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Gene expression</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Expresión genética</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Réaction chaîne polymérase RT</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Reverse transcription polymerase chain reaction</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Reacción cadena polimerasa transcripción inversa</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Gène</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Gene</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Gen</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Génétique</s0>
<s5>11</s5>
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<fC03 i1="07" i2="X" l="ENG"><s0>Genetics</s0>
<s5>11</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Genética</s0>
<s5>11</s5>
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<fC03 i1="08" i2="X" l="FRE"><s0>Puce électronique</s0>
<s5>12</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Chip</s0>
<s5>12</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Pulga electrónica</s0>
<s5>12</s5>
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<fC03 i1="09" i2="X" l="FRE"><s0>Analyse biochimique</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Biochemical analysis</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Análisis bioquímico</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Dosage</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Assay</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Dosificación</s0>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Biologie clinique</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Clinical biology</s0>
<s5>19</s5>
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<fC03 i1="13" i2="X" l="SPA"><s0>Biología clínica</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Biotechnologie</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Biotechnology</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Biotecnología</s0>
<s5>20</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>37</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>37</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>37</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>38</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>38</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>38</s5>
</fC07>
<fN21><s1>313</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
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Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

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