Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus
Identifieur interne : 000789 ( PascalFrancis/Corpus ); précédent : 000788; suivant : 000790Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus
Auteurs : Jyh-Lyh Juang ; Tsan-Chi Chen ; SHIH SHENG JIANG ; Chao A. Hsiung ; Wei-Chen Chen ; Guang-Wu Chen ; Shiang-Ming Lin ; Jih-Hui Lin ; Shu-Chun Chiu ; Yiu-Kay LaiSource :
- Laboratory investigation [ 0023-6837 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 04-0548676 INIST |
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ET : | Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus |
AU : | JUANG (Jyh-Lyh); CHEN (Tsan-Chi); SHIH SHENG JIANG; HSIUNG (Chao A.); CHEN (Wei-Chen); CHEN (Guang-Wu); LIN (Shiang-Ming); LIN (Jih-Hui); CHIU (Shu-Chun); LAI (Yiu-Kay) |
AF : | Division of Molecular and Genomic Medicine, National Health Research Institutes/Taipei/Taïwan (1 aut., 2 aut., 7 aut.); President's Laboratory, National Health Research Institutes/Taipei/Taïwan (3 aut.); Biostatistics and Bioinformatics, National Health Research Institutes/Taipei/Taïwan (4 aut., 5 aut., 6 aut.); Center for Disease Control, Department of Health/Taipei/Taïwan (8 aut., 9 aut.); Department of Life Science and Institute of Biotechnology, National Tsing Hua University/Hsinchu/Taïwan (10 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Laboratory investigation; ISSN 0023-6837; Coden LAINAW; Etats-Unis; Da. 2004; Vol. 84; No. 9; Pp. 1085-1091; Bibl. 17 ref. |
LA : | Anglais |
EA : | An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay. |
CC : | 002B24; 002A31; 215 |
FD : | Couplage; Réaction chaîne polymérase multiplex; Expression génique; RNA-directed DNA polymerase; Réaction chaîne polymérase RT; Gène; Génétique; Puce électronique; Analyse biochimique; Dosage; Syndrome respiratoire aigu sévère; Coronavirus; Biologie clinique; Biotechnologie |
FG : | Nucleotidyltransferases; Transferases; Enzyme; Virose; Infection; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Poumon pathologie |
ED : | Coupling; Multiplex polymerase chain reaction; Gene expression; RNA-directed DNA polymerase; Reverse transcription polymerase chain reaction; Gene; Genetics; Chip; Biochemical analysis; Assay; Severe acute respiratory syndrome; Coronavirus; Clinical biology; Biotechnology |
EG : | Nucleotidyltransferases; Transferases; Enzyme; Viral disease; Infection; Coronaviridae; Nidovirales; Virus; Respiratory disease; Lung disease |
SD : | Acoplamiento; Reacción cadena polimerasa multiplex; Expresión genética; RNA-directed DNA polymerase; Reacción cadena polimerasa transcripción inversa; Gen; Genética; Pulga electrónica; Análisis bioquímico; Dosificación; Síndrome respiratorio agudo severo; Coronavirus; Biología clínica; Biotecnología |
LO : | INIST-8078.354000110574040010 |
ID : | 04-0548676 |
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<front><div type="abstract" xml:lang="en">An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</div>
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<s5>11</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Genetics</s0>
<s5>11</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Genética</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Puce électronique</s0>
<s5>12</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Chip</s0>
<s5>12</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Pulga electrónica</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Analyse biochimique</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Biochemical analysis</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Análisis bioquímico</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Dosage</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Assay</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Dosificación</s0>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Biologie clinique</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Clinical biology</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Biología clínica</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Biotechnologie</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Biotechnology</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Biotecnología</s0>
<s5>20</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>37</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>37</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>37</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>38</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>38</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>38</s5>
</fC07>
<fN21><s1>313</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 04-0548676 INIST</NO>
<ET>Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus</ET>
<AU>JUANG (Jyh-Lyh); CHEN (Tsan-Chi); SHIH SHENG JIANG; HSIUNG (Chao A.); CHEN (Wei-Chen); CHEN (Guang-Wu); LIN (Shiang-Ming); LIN (Jih-Hui); CHIU (Shu-Chun); LAI (Yiu-Kay)</AU>
<AF>Division of Molecular and Genomic Medicine, National Health Research Institutes/Taipei/Taïwan (1 aut., 2 aut., 7 aut.); President's Laboratory, National Health Research Institutes/Taipei/Taïwan (3 aut.); Biostatistics and Bioinformatics, National Health Research Institutes/Taipei/Taïwan (4 aut., 5 aut., 6 aut.); Center for Disease Control, Department of Health/Taipei/Taïwan (8 aut., 9 aut.); Department of Life Science and Institute of Biotechnology, National Tsing Hua University/Hsinchu/Taïwan (10 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Laboratory investigation; ISSN 0023-6837; Coden LAINAW; Etats-Unis; Da. 2004; Vol. 84; No. 9; Pp. 1085-1091; Bibl. 17 ref.</SO>
<LA>Anglais</LA>
<EA>An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</EA>
<CC>002B24; 002A31; 215</CC>
<FD>Couplage; Réaction chaîne polymérase multiplex; Expression génique; RNA-directed DNA polymerase; Réaction chaîne polymérase RT; Gène; Génétique; Puce électronique; Analyse biochimique; Dosage; Syndrome respiratoire aigu sévère; Coronavirus; Biologie clinique; Biotechnologie</FD>
<FG>Nucleotidyltransferases; Transferases; Enzyme; Virose; Infection; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Poumon pathologie</FG>
<ED>Coupling; Multiplex polymerase chain reaction; Gene expression; RNA-directed DNA polymerase; Reverse transcription polymerase chain reaction; Gene; Genetics; Chip; Biochemical analysis; Assay; Severe acute respiratory syndrome; Coronavirus; Clinical biology; Biotechnology</ED>
<EG>Nucleotidyltransferases; Transferases; Enzyme; Viral disease; Infection; Coronaviridae; Nidovirales; Virus; Respiratory disease; Lung disease</EG>
<SD>Acoplamiento; Reacción cadena polimerasa multiplex; Expresión genética; RNA-directed DNA polymerase; Reacción cadena polimerasa transcripción inversa; Gen; Genética; Pulga electrónica; Análisis bioquímico; Dosificación; Síndrome respiratorio agudo severo; Coronavirus; Biología clínica; Biotecnología</SD>
<LO>INIST-8078.354000110574040010</LO>
<ID>04-0548676</ID>
</server>
</inist>
</record>
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