SrasV1, PascalFrancis, Curation, bibRecord, 000102

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

Identifieur interne : 000102 ( PascalFrancis/Curation ); précédent : 000101; suivant : 000103

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

Auteurs : Leo L. M. Poon [Hong Kong] ; Bonnie W. Y. Wong [Hong Kong] ; KWOK HUNG CHAN [Hong Kong] ; Cynthia S. W. Leung [Hong Kong] ; KWOK YUNG YUEN [Hong Kong] ; YI GUAN [Hong Kong] ; Joseph S. M. Peiris [Hong Kong]

Source :

Journal of clinical virology [ 1386-6532 ] ; 2004.

RBID : Pascal:04-0287118

Descripteurs français

Pascal (Inist)
- Coronavirus, Analyse quantitative, Réaction chaîne polymérase RT, Détection, Contrôle, Réaction chaîne polymérase, Inhibiteur, Précoce, Diagnostic, Syndrome respiratoire aigu sévère, Microbiologie, Virologie.
Wicri :
- topic : Analyse quantitative.

English descriptors

KwdEn :
- Check, Coronavirus, Detection, Diagnosis, Early, Inhibitor, Microbiology, Polymerase chain reaction, Quantitative analysis, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome, Virology.

Abstract

In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.

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A03		`1`		`@0 J. clin. virol.`
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A08	`01`	`1`	`ENG`	`@1 A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors`
A11	`01`	`1`		`@1 POON (Leo L. M.)`
A11	`02`	`1`		`@1 WONG (Bonnie W. Y.)`
A11	`03`	`1`		`@1 KWOK HUNG CHAN`
A11	`04`	`1`		`@1 LEUNG (Cynthia S. W.)`
A11	`05`	`1`		`@1 KWOK YUNG YUEN`
A11	`06`	`1`		`@1 YI GUAN`
A11	`07`	`1`		`@1 PEIRIS (Joseph S. M.)`
A14	`01`			`@1 Department of Microbiology, Queen Mary Hospital, University of Hong Kong @2 Pokfulam SAR @3 HKG @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut.`
A14	`02`			`@1 Department of Microbiology, Queen Mary Hospital @2 SAR @3 HKG @Z 3 aut.`
A20				`@1 214-217`
A21				`@1 2004`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 26272 @5 354000110266800030`
A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
A45				`@0 7 ref.`
A47	`01`	`1`		`@0 04-0287118`
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A64	`01`	`1`		`@0 Journal of clinical virology`
A66	`01`			`@0 NLD`
C01	`01`		`ENG`	@0 In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.
C02	`01`	`X`		`@0 002A05C10`
C02	`02`	`X`		`@0 002B05C02J`
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C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
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C03	`02`	`X`	`FRE`	`@0 Analyse quantitative @5 05`
C03	`02`	`X`	`ENG`	`@0 Quantitative analysis @5 05`
C03	`02`	`X`	`SPA`	`@0 Análisis cuantitativo @5 05`
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C03	`03`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 06`
C03	`03`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 06`
C03	`04`	`X`	`FRE`	`@0 Détection @5 07`
C03	`04`	`X`	`ENG`	`@0 Detection @5 07`
C03	`04`	`X`	`SPA`	`@0 Detección @5 07`
C03	`05`	`X`	`FRE`	`@0 Contrôle @5 08`
C03	`05`	`X`	`ENG`	`@0 Check @5 08`
C03	`05`	`X`	`SPA`	`@0 Control @5 08`
C03	`06`	`X`	`FRE`	`@0 Réaction chaîne polymérase @5 09`
C03	`06`	`X`	`ENG`	`@0 Polymerase chain reaction @5 09`
C03	`06`	`X`	`SPA`	`@0 Reacción cadena polimerasa @5 09`
C03	`07`	`X`	`FRE`	`@0 Inhibiteur @5 10`
C03	`07`	`X`	`ENG`	`@0 Inhibitor @5 10`
C03	`07`	`X`	`SPA`	`@0 Inhibidor @5 10`
C03	`08`	`X`	`FRE`	`@0 Précoce @5 11`
C03	`08`	`X`	`ENG`	`@0 Early @5 11`
C03	`08`	`X`	`SPA`	`@0 Precoz @5 11`
C03	`09`	`X`	`FRE`	`@0 Diagnostic @5 12`
C03	`09`	`X`	`ENG`	`@0 Diagnosis @5 12`
C03	`09`	`X`	`SPA`	`@0 Diagnóstico @5 12`
C03	`10`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`10`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`10`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C03	`11`	`X`	`FRE`	`@0 Microbiologie @5 67`
C03	`11`	`X`	`ENG`	`@0 Microbiology @5 67`
C03	`11`	`X`	`SPA`	`@0 Microbiología @5 67`
C03	`12`	`X`	`FRE`	`@0 Virologie @5 68`
C03	`12`	`X`	`ENG`	`@0 Virology @5 68`
C03	`12`	`X`	`SPA`	`@0 Virología @5 68`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Virose @2 NM`
C07	`04`	`X`	`ENG`	`@0 Viral disease @2 NM`
C07	`04`	`X`	`SPA`	`@0 Virosis @2 NM`
C07	`05`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`05`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`05`	`X`	`SPA`	`@0 Infección @2 NM`
C07	`06`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 19`
C07	`06`	`X`	`ENG`	`@0 Respiratory disease @5 19`
C07	`06`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 19`
C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 20`
C07	`07`	`X`	`ENG`	`@0 Lung disease @5 20`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 20`
N21				`@1 180`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Pascal:04-0287118

Le document en format XML

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<series><title level="j" type="main">Journal of clinical virology</title>
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<front><div type="abstract" xml:lang="en">In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.</div>
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<fC01 i1="01" l="ENG"><s0>In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B05C02J</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Analyse quantitative</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Quantitative analysis</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Análisis cuantitativo</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Réaction chaîne polymérase RT</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Reverse transcription polymerase chain reaction</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Reacción cadena polimerasa transcripción inversa</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Détection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Detection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Detección</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Contrôle</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Check</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Control</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Réaction chaîne polymérase</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Polymerase chain reaction</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Reacción cadena polimerasa</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Inhibiteur</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Inhibitor</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Inhibidor</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Précoce</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Early</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Precoz</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Diagnostic</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Diagnosis</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Diagnóstico</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>67</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>67</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>67</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Virologie</s0>
<s5>68</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Virology</s0>
<s5>68</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Virología</s0>
<s5>68</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>19</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>19</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>19</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>20</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>20</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>20</s5>
</fC07>
<fN21><s1>180</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

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