SrasV1, PascalFrancis, Curation, bibRecord, 000009

Identification of a novel coronavirus in patients with severe acute respiratory syndrome

Identifieur interne : 000009 ( PascalFrancis/Curation ); précédent : 000008; suivant : 000010

Identification of a novel coronavirus in patients with severe acute respiratory syndrome

Auteurs : Christian Drosten [Allemagne] ; Stephan Günther [Allemagne] ; Wolfgang Preiser [Allemagne] ; Sylvie Van Der Werf ; Hans-Reinhard Brodt [Allemagne] ; Stephan Becker [Allemagne] ; Holger Rabenau [Allemagne] ; Marcus Panning [Allemagne] ; Larissa Kolesnikova [Allemagne] ; Ron A. M. Fouchier [Pays-Bas] ; Annemarie Berger [Allemagne] ; Ana-Maria Burguiere [France] ; Jindrich Cinatl [Allemagne] ; Markus Eickmann [Allemagne] ; Nicolas Escriou [France] ; Klaus Grywna [Allemagne] ; Stefanie Kramme [Allemagne] ; Jean-Claude Manuguerra [France] ; Stefanie Müller [Allemagne] ; Volker Rickerts [Allemagne] ; Martin Stürmer [Allemagne] ; Simon Vieth [Allemagne] ; Hans-Dieter Klenk [Allemagne] ; Albert D. M. E. Osterhaus [Pays-Bas] ; Herbert Schmitz [Allemagne] ; Hans Wilhelm Doerr [Allemagne]

Source :

The New England journal of medicine [ 0028-4793 ] ; 2003.

RBID : Pascal:03-0295886

Descripteurs français

Pascal (Inist)
- Poumon pathologie, Détresse respiratoire adulte syndrome, Virose, Homme, Coronavirus, Exploration microbiologique, Identification, Pathogénie, Prélèvement, Expectoration, Plasma sanguin, Fèces, Asie, SRAS, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Adult respiratory distress syndrome, Asia, Blood plasma, Coronavirus, Feces, Human, Identification, Lung disease, Microbiological investigation, Pathogenesis, Samplings, Severe acute respiratory syndrome, Sputum, Viral disease.

Abstract

BACKGROUND The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent ofthe nucleotide sequence). On the basis ofthe obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated. CONCLUSIONS The novel coronavirus might have a role in causing SARS.

A01	`01`	`1`		`@0 0028-4793`
A02	`01`			`@0 NEJMAG`
A03		`1`		`@0 N. Engl. j. med.`
A05				`@2 348`
A06				`@2 20`
A08	`01`	`1`	`ENG`	`@1 Identification of a novel coronavirus in patients with severe acute respiratory syndrome`
A11	`01`	`1`		`@1 DROSTEN (Christian)`
A11	`02`	`1`		`@1 GÜNTHER (Stephan)`
A11	`03`	`1`		`@1 PREISER (Wolfgang)`
A11	`04`	`1`		`@1 VAN DER WERF (Sylvie)`
A11	`05`	`1`		`@1 BRODT (Hans-Reinhard)`
A11	`06`	`1`		`@1 BECKER (Stephan)`
A11	`07`	`1`		`@1 RABENAU (Holger)`
A11	`08`	`1`		`@1 PANNING (Marcus)`
A11	`09`	`1`		`@1 KOLESNIKOVA (Larissa)`
A11	`10`	`1`		`@1 FOUCHIER (Ron A. M.)`
A11	`11`	`1`		`@1 BERGER (Annemarie)`
A11	`12`	`1`		`@1 BURGUIERE (Ana-Maria)`
A11	`13`	`1`		`@1 CINATL (Jindrich)`
A11	`14`	`1`		`@1 EICKMANN (Markus)`
A11	`15`	`1`		`@1 ESCRIOU (Nicolas)`
A11	`16`	`1`		`@1 GRYWNA (Klaus)`
A11	`17`	`1`		`@1 KRAMME (Stefanie)`
A11	`18`	`1`		`@1 MANUGUERRA (Jean-Claude)`
A11	`19`	`1`		`@1 MÜLLER (Stefanie)`
A11	`20`	`1`		`@1 RICKERTS (Volker)`
A11	`21`	`1`		`@1 STÜRMER (Martin)`
A11	`22`	`1`		`@1 VIETH (Simon)`
A11	`23`	`1`		`@1 KLENK (Hans-Dieter)`
A11	`24`	`1`		`@1 OSTERHAUS (Albert D. M. E.)`
A11	`25`	`1`		`@1 SCHMITZ (Herbert)`
A11	`26`	`1`		`@1 DOERR (Hans Wilhelm)`
A14	`01`			`@1 Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases @2 Hamburg @3 DEU @Z 1 aut. @Z 2 aut. @Z 8 aut. @Z 16 aut. @Z 17 aut. @Z 19 aut. @Z 22 aut. @Z 25 aut.`
A14	`02`			`@1 Institute of Medical Virology, Johann Wolfgang Goethe University @2 Frankfurt am Main @3 DEU @Z 3 aut. @Z 7 aut. @Z 11 aut. @Z 13 aut. @Z 21 aut. @Z 26 aut.`
A14	`03`			`@1 Medical Clinic III, Johann Wolfgang Goethe University @2 Frankfurt am Main @3 DEU @Z 5 aut. @Z 20 aut.`
A14	`04`			`@1 Institute of Virology, Philipps University @2 Marburg @3 DEU @Z 6 aut. @Z 9 aut. @Z 14 aut. @Z 23 aut.`
A14	`05`			`@1 Institute of Virology, Erasmus University @2 Rotterdam @3 NLD @Z 10 aut. @Z 24 aut.`
A14	`06`			`@1 Pasteur Institute, Molecular Genetics of Respiratory Tract Viruses, National Influenza Center @2 Paris @3 FRA @Z 12 aut. @Z 15 aut. @Z 18 aut.`
A20				`@1 1967-1976`
A21				`@1 2003`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 6013 @5 354000118185320050`
A44				`@0 0000 @1 © 2003 INIST-CNRS. All rights reserved.`
A45				`@0 13 ref.`
A47	`01`	`1`		`@0 03-0295886`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 The New England journal of medicine`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 BACKGROUND The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent ofthe nucleotide sequence). On the basis ofthe obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated. CONCLUSIONS The novel coronavirus might have a role in causing SARS.
C02	`01`	`X`		`@0 002B05C02C`
C02	`02`	`X`		`@0 235`
C03	`01`	`X`	`FRE`	`@0 Poumon pathologie @5 01`
C03	`01`	`X`	`ENG`	`@0 Lung disease @5 01`
C03	`01`	`X`	`SPA`	`@0 Pulmón patología @5 01`
C03	`02`	`X`	`FRE`	`@0 Détresse respiratoire adulte syndrome @2 NM @5 02`
C03	`02`	`X`	`ENG`	`@0 Adult respiratory distress syndrome @2 NM @5 02`
C03	`02`	`X`	`SPA`	`@0 Trastorno respiratorio adulto síndrome @2 NM @5 02`
C03	`03`	`X`	`FRE`	`@0 Virose @5 03`
C03	`03`	`X`	`ENG`	`@0 Viral disease @5 03`
C03	`03`	`X`	`SPA`	`@0 Virosis @5 03`
C03	`04`	`X`	`FRE`	`@0 Homme @5 04`
C03	`04`	`X`	`ENG`	`@0 Human @5 04`
C03	`04`	`X`	`SPA`	`@0 Hombre @5 04`
C03	`05`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 05`
C03	`05`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 05`
C03	`05`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 05`
C03	`06`	`X`	`FRE`	`@0 Exploration microbiologique @5 06`
C03	`06`	`X`	`ENG`	`@0 Microbiological investigation @5 06`
C03	`06`	`X`	`SPA`	`@0 Análisis microbiológico @5 06`
C03	`07`	`X`	`FRE`	`@0 Identification @5 07`
C03	`07`	`X`	`ENG`	`@0 Identification @5 07`
C03	`07`	`X`	`SPA`	`@0 Identificación @5 07`
C03	`08`	`X`	`FRE`	`@0 Pathogénie @5 08`
C03	`08`	`X`	`ENG`	`@0 Pathogenesis @5 08`
C03	`08`	`X`	`SPA`	`@0 Patogenia @5 08`
C03	`09`	`X`	`FRE`	`@0 Prélèvement @5 09`
C03	`09`	`X`	`ENG`	`@0 Samplings @5 09`
C03	`09`	`X`	`SPA`	`@0 Toma de muestra @5 09`
C03	`10`	`X`	`FRE`	`@0 Expectoration @5 10`
C03	`10`	`X`	`ENG`	`@0 Sputum @5 10`
C03	`10`	`X`	`SPA`	`@0 Expectoración @5 10`
C03	`11`	`X`	`FRE`	`@0 Plasma sanguin @5 11`
C03	`11`	`X`	`ENG`	`@0 Blood plasma @5 11`
C03	`11`	`X`	`SPA`	`@0 Plasma sanguíneo @5 11`
C03	`12`	`X`	`FRE`	`@0 Fèces @5 12`
C03	`12`	`X`	`ENG`	`@0 Feces @5 12`
C03	`12`	`X`	`SPA`	`@0 Heces @5 12`
C03	`13`	`X`	`FRE`	`@0 Asie @2 NG @5 13`
C03	`13`	`X`	`ENG`	`@0 Asia @2 NG @5 13`
C03	`13`	`X`	`SPA`	`@0 Asia @2 NG @5 13`
C03	`14`	`X`	`FRE`	`@0 SRAS @4 INC @5 86`
C03	`15`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @4 CD @5 96`
C03	`15`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Infection`
C07	`01`	`X`	`ENG`	`@0 Infection`
C07	`01`	`X`	`SPA`	`@0 Infección`
C07	`02`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`04`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`04`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`04`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`05`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 37`
C07	`05`	`X`	`ENG`	`@0 Respiratory disease @5 37`
C07	`05`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 37`
N21				`@1 195`
N82				`@1 PSI`

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Pascal:03-0295886

Le document en format XML

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<author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
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<author><name sortKey="Kolesnikova, Larissa" sort="Kolesnikova, Larissa" uniqKey="Kolesnikova L" first="Larissa" last="Kolesnikova">Larissa Kolesnikova</name>
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<author><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A. M." last="Fouchier">Ron A. M. Fouchier</name>
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<author><name sortKey="Berger, Annemarie" sort="Berger, Annemarie" uniqKey="Berger A" first="Annemarie" last="Berger">Annemarie Berger</name>
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<author><name sortKey="Burguiere, Ana Maria" sort="Burguiere, Ana Maria" uniqKey="Burguiere A" first="Ana-Maria" last="Burguiere">Ana-Maria Burguiere</name>
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<author><name sortKey="Cinatl, Jindrich" sort="Cinatl, Jindrich" uniqKey="Cinatl J" first="Jindrich" last="Cinatl">Jindrich Cinatl</name>
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<author><name sortKey="Eickmann, Markus" sort="Eickmann, Markus" uniqKey="Eickmann M" first="Markus" last="Eickmann">Markus Eickmann</name>
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<author><name sortKey="Escriou, Nicolas" sort="Escriou, Nicolas" uniqKey="Escriou N" first="Nicolas" last="Escriou">Nicolas Escriou</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Pasteur Institute, Molecular Genetics of Respiratory Tract Viruses, National Influenza Center</s1>
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<author><name sortKey="Grywna, Klaus" sort="Grywna, Klaus" uniqKey="Grywna K" first="Klaus" last="Grywna">Klaus Grywna</name>
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<author><name sortKey="Kramme, Stefanie" sort="Kramme, Stefanie" uniqKey="Kramme S" first="Stefanie" last="Kramme">Stefanie Kramme</name>
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<author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Pasteur Institute, Molecular Genetics of Respiratory Tract Viruses, National Influenza Center</s1>
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<author><name sortKey="Muller, Stefanie" sort="Muller, Stefanie" uniqKey="Muller S" first="Stefanie" last="Müller">Stefanie Müller</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases</s1>
<s2>Hamburg</s2>
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<author><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D. M. E." last="Osterhaus">Albert D. M. E. Osterhaus</name>
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<author><name sortKey="Doerr, Hans Wilhelm" sort="Doerr, Hans Wilhelm" uniqKey="Doerr H" first="Hans Wilhelm" last="Doerr">Hans Wilhelm Doerr</name>
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<publicationStmt><idno type="wicri:source">INIST</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification of a novel coronavirus in patients with severe acute respiratory syndrome</title>
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<s2>Hamburg</s2>
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</author>
<author><name sortKey="Gunther, Stephan" sort="Gunther, Stephan" uniqKey="Gunther S" first="Stephan" last="Günther">Stephan Günther</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases</s1>
<s2>Hamburg</s2>
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</author>
<author><name sortKey="Preiser, Wolfgang" sort="Preiser, Wolfgang" uniqKey="Preiser W" first="Wolfgang" last="Preiser">Wolfgang Preiser</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Medical Virology, Johann Wolfgang Goethe University</s1>
<s2>Frankfurt am Main</s2>
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<country>Allemagne</country>
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<author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name>
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<author><name sortKey="Brodt, Hans Reinhard" sort="Brodt, Hans Reinhard" uniqKey="Brodt H" first="Hans-Reinhard" last="Brodt">Hans-Reinhard Brodt</name>
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</author>
<author><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Virology, Philipps University</s1>
<s2>Marburg</s2>
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</inist:fA14>
<country>Allemagne</country>
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</author>
<author><name sortKey="Rabenau, Holger" sort="Rabenau, Holger" uniqKey="Rabenau H" first="Holger" last="Rabenau">Holger Rabenau</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Medical Virology, Johann Wolfgang Goethe University</s1>
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<author><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name>
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</author>
<author><name sortKey="Kolesnikova, Larissa" sort="Kolesnikova, Larissa" uniqKey="Kolesnikova L" first="Larissa" last="Kolesnikova">Larissa Kolesnikova</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Virology, Philipps University</s1>
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</author>
<author><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A. M." last="Fouchier">Ron A. M. Fouchier</name>
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<author><name sortKey="Burguiere, Ana Maria" sort="Burguiere, Ana Maria" uniqKey="Burguiere A" first="Ana-Maria" last="Burguiere">Ana-Maria Burguiere</name>
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<author><name sortKey="Eickmann, Markus" sort="Eickmann, Markus" uniqKey="Eickmann M" first="Markus" last="Eickmann">Markus Eickmann</name>
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<author><name sortKey="Escriou, Nicolas" sort="Escriou, Nicolas" uniqKey="Escriou N" first="Nicolas" last="Escriou">Nicolas Escriou</name>
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<author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name>
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<author><name sortKey="Muller, Stefanie" sort="Muller, Stefanie" uniqKey="Muller S" first="Stefanie" last="Müller">Stefanie Müller</name>
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<author><name sortKey="Rickerts, Volker" sort="Rickerts, Volker" uniqKey="Rickerts V" first="Volker" last="Rickerts">Volker Rickerts</name>
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<series><title level="j" type="main">The New England journal of medicine</title>
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<term>Blood plasma</term>
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<term>Feces</term>
<term>Human</term>
<term>Identification</term>
<term>Lung disease</term>
<term>Microbiological investigation</term>
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<term>Samplings</term>
<term>Severe acute respiratory syndrome</term>
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<term>Détresse respiratoire adulte syndrome</term>
<term>Virose</term>
<term>Homme</term>
<term>Coronavirus</term>
<term>Exploration microbiologique</term>
<term>Identification</term>
<term>Pathogénie</term>
<term>Prélèvement</term>
<term>Expectoration</term>
<term>Plasma sanguin</term>
<term>Fèces</term>
<term>Asie</term>
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<front><div type="abstract" xml:lang="en">BACKGROUND The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent ofthe nucleotide sequence). On the basis ofthe obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated. CONCLUSIONS The novel coronavirus might have a role in causing SARS.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0028-4793</s0>
</fA01>
<fA02 i1="01"><s0>NEJMAG</s0>
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<fA03 i2="1"><s0>N. Engl. j. med.</s0>
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<fA05><s2>348</s2>
</fA05>
<fA06><s2>20</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Identification of a novel coronavirus in patients with severe acute respiratory syndrome</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>DROSTEN (Christian)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>GÜNTHER (Stephan)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>PREISER (Wolfgang)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>VAN DER WERF (Sylvie)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>BRODT (Hans-Reinhard)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BECKER (Stephan)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>RABENAU (Holger)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>PANNING (Marcus)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>KOLESNIKOVA (Larissa)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>FOUCHIER (Ron A. M.)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>BERGER (Annemarie)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>BURGUIERE (Ana-Maria)</s1>
</fA11>
<fA11 i1="13" i2="1"><s1>CINATL (Jindrich)</s1>
</fA11>
<fA11 i1="14" i2="1"><s1>EICKMANN (Markus)</s1>
</fA11>
<fA11 i1="15" i2="1"><s1>ESCRIOU (Nicolas)</s1>
</fA11>
<fA11 i1="16" i2="1"><s1>GRYWNA (Klaus)</s1>
</fA11>
<fA11 i1="17" i2="1"><s1>KRAMME (Stefanie)</s1>
</fA11>
<fA11 i1="18" i2="1"><s1>MANUGUERRA (Jean-Claude)</s1>
</fA11>
<fA11 i1="19" i2="1"><s1>MÜLLER (Stefanie)</s1>
</fA11>
<fA11 i1="20" i2="1"><s1>RICKERTS (Volker)</s1>
</fA11>
<fA11 i1="21" i2="1"><s1>STÜRMER (Martin)</s1>
</fA11>
<fA11 i1="22" i2="1"><s1>VIETH (Simon)</s1>
</fA11>
<fA11 i1="23" i2="1"><s1>KLENK (Hans-Dieter)</s1>
</fA11>
<fA11 i1="24" i2="1"><s1>OSTERHAUS (Albert D. M. E.)</s1>
</fA11>
<fA11 i1="25" i2="1"><s1>SCHMITZ (Herbert)</s1>
</fA11>
<fA11 i1="26" i2="1"><s1>DOERR (Hans Wilhelm)</s1>
</fA11>
<fA14 i1="01"><s1>Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases</s1>
<s2>Hamburg</s2>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>16 aut.</sZ>
<sZ>17 aut.</sZ>
<sZ>19 aut.</sZ>
<sZ>22 aut.</sZ>
<sZ>25 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Institute of Medical Virology, Johann Wolfgang Goethe University</s1>
<s2>Frankfurt am Main</s2>
<s3>DEU</s3>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
<sZ>21 aut.</sZ>
<sZ>26 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Medical Clinic III, Johann Wolfgang Goethe University</s1>
<s2>Frankfurt am Main</s2>
<s3>DEU</s3>
<sZ>5 aut.</sZ>
<sZ>20 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Institute of Virology, Philipps University</s1>
<s2>Marburg</s2>
<s3>DEU</s3>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>14 aut.</sZ>
<sZ>23 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Institute of Virology, Erasmus University</s1>
<s2>Rotterdam</s2>
<s3>NLD</s3>
<sZ>10 aut.</sZ>
<sZ>24 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Pasteur Institute, Molecular Genetics of Respiratory Tract Viruses, National Influenza Center</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>12 aut.</sZ>
<sZ>15 aut.</sZ>
<sZ>18 aut.</sZ>
</fA14>
<fA20><s1>1967-1976</s1>
</fA20>
<fA21><s1>2003</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>6013</s2>
<s5>354000118185320050</s5>
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<fA44><s0>0000</s0>
<s1>© 2003 INIST-CNRS. All rights reserved.</s1>
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<fA45><s0>13 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>03-0295886</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>The New England journal of medicine</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>BACKGROUND The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent. METHODS Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques. RESULTS A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent ofthe nucleotide sequence). On the basis ofthe obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated. CONCLUSIONS The novel coronavirus might have a role in causing SARS.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B05C02C</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>235</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Poumon pathologie</s0>
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<fC03 i1="01" i2="X" l="ENG"><s0>Lung disease</s0>
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<fC03 i1="01" i2="X" l="SPA"><s0>Pulmón patología</s0>
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<s5>02</s5>
</fC03>
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<s2>NM</s2>
<s5>02</s5>
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<fC03 i1="03" i2="X" l="FRE"><s0>Virose</s0>
<s5>03</s5>
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<fC03 i1="03" i2="X" l="SPA"><s0>Virosis</s0>
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<fC03 i1="04" i2="X" l="FRE"><s0>Homme</s0>
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<s5>04</s5>
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<fC03 i1="05" i2="X" l="FRE"><s0>Coronavirus</s0>
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<s5>05</s5>
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<fC03 i1="05" i2="X" l="ENG"><s0>Coronavirus</s0>
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<s5>05</s5>
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<fC03 i1="05" i2="X" l="SPA"><s0>Coronavirus</s0>
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<fC03 i1="07" i2="X" l="ENG"><s0>Identification</s0>
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<s5>09</s5>
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<s5>09</s5>
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<s5>10</s5>
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<fC03 i1="10" i2="X" l="SPA"><s0>Expectoración</s0>
<s5>10</s5>
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<fC03 i1="11" i2="X" l="FRE"><s0>Plasma sanguin</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Blood plasma</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Plasma sanguíneo</s0>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Fèces</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Feces</s0>
<s5>12</s5>
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<fC03 i1="12" i2="X" l="SPA"><s0>Heces</s0>
<s5>12</s5>
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<fC03 i1="13" i2="X" l="FRE"><s0>Asie</s0>
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<s5>13</s5>
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<s5>13</s5>
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<fC03 i1="13" i2="X" l="SPA"><s0>Asia</s0>
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<s5>13</s5>
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<fC03 i1="14" i2="X" l="FRE"><s0>SRAS</s0>
<s4>INC</s4>
<s5>86</s5>
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<fC03 i1="15" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s4>CD</s4>
<s5>96</s5>
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<fC07 i1="01" i2="X" l="FRE"><s0>Infection</s0>
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<fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
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<s2>NW</s2>
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<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
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<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
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<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
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<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
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<s5>37</s5>
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<s5>37</s5>
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<fC07 i1="05" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>37</s5>
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<fN21><s1>195</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
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Identification of a novel coronavirus in patients with severe acute respiratory syndrome

Identification of a novel coronavirus in patients with severe acute respiratory syndrome

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