SrasV1, PascalFrancis, Corpus, bibRecord, 000525

Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR

Identifieur interne : 000525 ( PascalFrancis/Corpus ); précédent : 000524; suivant : 000526

Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR

Auteurs : Maria Cristina Keightley ; Peter Sillekens ; Wim Schippers ; Charles Rinaldo ; Kirsten St. George

Source :

Journal of medical virology [ 0146-6615 ] ; 2005.

RBID : Pascal:06-0194439

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Temps réel, Détection, Réaction chaîne polymérase RT, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Detection, Real time, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.

Abstract

Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR`
A11	`01`	`1`		`@1 KEIGHTLEY (Maria Cristina)`
A11	`02`	`1`		`@1 SILLEKENS (Peter)`
A11	`03`	`1`		`@1 SCHIPPERS (Wim)`
A11	`04`	`1`		`@1 RINALDO (Charles)`
A11	`05`	`1`		`@1 GEORGE (Kirsten St.)`
A14	`01`			`@1 Clinical Virology Laboratory, University of Pittsburgh Medical Center @2 Pittsburgh, Pennsylvania @3 USA @Z 1 aut. @Z 4 aut. @Z 5 aut.`
A14	`02`			`@1 Department of Pathology, University of Pittsburgh @2 Pittsburgh, Pennsylvania @3 USA @Z 1 aut. @Z 4 aut. @Z 5 aut.`
A14	`03`			`@1 Department of bioMérieux @2 Boxtel @3 NLD @Z 2 aut. @Z 3 aut.`
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A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 17422 @5 354000131964810220`
A44				`@0 0000 @1 © 2006 INIST-CNRS. All rights reserved.`
A45				`@0 25 ref.`
A47	`01`	`1`		`@0 06-0194439`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of medical virology`
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C01	`01`		`ENG`	@0 Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.
C02	`01`	`X`		`@0 002A05C10`
C02	`02`	`X`		`@0 002B05C02J`
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C03	`01`	`X`	`ENG`	`@0 Severe acute respiratory syndrome virus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Severe acute respiratory syndrome virus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Temps réel @5 05`
C03	`02`	`X`	`ENG`	`@0 Real time @5 05`
C03	`02`	`X`	`SPA`	`@0 Tiempo real @5 05`
C03	`03`	`X`	`FRE`	`@0 Détection @5 06`
C03	`03`	`X`	`ENG`	`@0 Detection @5 06`
C03	`03`	`X`	`SPA`	`@0 Detección @5 06`
C03	`04`	`X`	`FRE`	`@0 Réaction chaîne polymérase RT @5 07`
C03	`04`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 07`
C03	`04`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 07`
C03	`05`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`05`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`05`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
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C07	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW`
C07	`02`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`04`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`04`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`04`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`05`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 13`
C07	`05`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`05`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`06`	`X`	`FRE`	`@0 Virose`
C07	`06`	`X`	`ENG`	`@0 Viral disease`
C07	`06`	`X`	`SPA`	`@0 Virosis`
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C07	`08`	`X`	`FRE`	`@0 Poumon pathologie @5 16`
C07	`08`	`X`	`ENG`	`@0 Lung disease @5 16`
C07	`08`	`X`	`SPA`	`@0 Pulmón patología @5 16`
N21				`@1 121`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 06-0194439 INIST
ET :	Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR
AU :	KEIGHTLEY (Maria Cristina); SILLEKENS (Peter); SCHIPPERS (Wim); RINALDO (Charles); GEORGE (Kirsten St.)
AF :	Clinical Virology Laboratory, University of Pittsburgh Medical Center/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of Pathology, University of Pittsburgh/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of bioMérieux/Boxtel/Pays-Bas (2 aut., 3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 4; Pp. 602-608; Bibl. 25 ref.
LA :	Anglais
EA :	Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.
CC :	002A05C10; 002B05C02J
FD :	Virus syndrome respiratoire aigu sévère; Temps réel; Détection; Réaction chaîne polymérase RT; Syndrome respiratoire aigu sévère
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Severe acute respiratory syndrome virus; Real time; Detection; Reverse transcription polymerase chain reaction; Severe acute respiratory syndrome
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Severe acute respiratory syndrome virus; Tiempo real; Detección; Reacción cadena polimerasa transcripción inversa; Síndrome respiratorio agudo severo
LO :	INIST-17422.354000131964810220
ID :	06-0194439

Links to Exploration step

Pascal:06-0194439

Le document en format XML

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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.</div>
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<server><NO>PASCAL 06-0194439 INIST</NO>
<ET>Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR</ET>
<AU>KEIGHTLEY (Maria Cristina); SILLEKENS (Peter); SCHIPPERS (Wim); RINALDO (Charles); GEORGE (Kirsten St.)</AU>
<AF>Clinical Virology Laboratory, University of Pittsburgh Medical Center/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of Pathology, University of Pittsburgh/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of bioMérieux/Boxtel/Pays-Bas (2 aut., 3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 4; Pp. 602-608; Bibl. 25 ref.</SO>
<LA>Anglais</LA>
<EA>Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.</EA>
<CC>002A05C10; 002B05C02J</CC>
<FD>Virus syndrome respiratoire aigu sévère; Temps réel; Détection; Réaction chaîne polymérase RT; Syndrome respiratoire aigu sévère</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Severe acute respiratory syndrome virus; Real time; Detection; Reverse transcription polymerase chain reaction; Severe acute respiratory syndrome</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Severe acute respiratory syndrome virus; Tiempo real; Detección; Reacción cadena polimerasa transcripción inversa; Síndrome respiratorio agudo severo</SD>
<LO>INIST-17422.354000131964810220</LO>
<ID>06-0194439</ID>
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Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR

Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR

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