Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR
Identifieur interne : 000525 ( PascalFrancis/Corpus ); précédent : 000524; suivant : 000526Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR
Auteurs : Maria Cristina Keightley ; Peter Sillekens ; Wim Schippers ; Charles Rinaldo ; Kirsten St. GeorgeSource :
- Journal of medical virology [ 0146-6615 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 06-0194439 INIST |
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ET : | Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR |
AU : | KEIGHTLEY (Maria Cristina); SILLEKENS (Peter); SCHIPPERS (Wim); RINALDO (Charles); GEORGE (Kirsten St.) |
AF : | Clinical Virology Laboratory, University of Pittsburgh Medical Center/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of Pathology, University of Pittsburgh/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of bioMérieux/Boxtel/Pays-Bas (2 aut., 3 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 4; Pp. 602-608; Bibl. 25 ref. |
LA : | Anglais |
EA : | Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV. |
CC : | 002A05C10; 002B05C02J |
FD : | Virus syndrome respiratoire aigu sévère; Temps réel; Détection; Réaction chaîne polymérase RT; Syndrome respiratoire aigu sévère |
FG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie |
ED : | Severe acute respiratory syndrome virus; Real time; Detection; Reverse transcription polymerase chain reaction; Severe acute respiratory syndrome |
EG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Severe acute respiratory syndrome virus; Tiempo real; Detección; Reacción cadena polimerasa transcripción inversa; Síndrome respiratorio agudo severo |
LO : | INIST-17422.354000131964810220 |
ID : | 06-0194439 |
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Pascal:06-0194439Le document en format XML
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.</div>
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<server><NO>PASCAL 06-0194439 INIST</NO>
<ET>Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR</ET>
<AU>KEIGHTLEY (Maria Cristina); SILLEKENS (Peter); SCHIPPERS (Wim); RINALDO (Charles); GEORGE (Kirsten St.)</AU>
<AF>Clinical Virology Laboratory, University of Pittsburgh Medical Center/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of Pathology, University of Pittsburgh/Pittsburgh, Pennsylvania/Etats-Unis (1 aut., 4 aut., 5 aut.); Department of bioMérieux/Boxtel/Pays-Bas (2 aut., 3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 4; Pp. 602-608; Bibl. 25 ref.</SO>
<LA>Anglais</LA>
<EA>Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.</EA>
<CC>002A05C10; 002B05C02J</CC>
<FD>Virus syndrome respiratoire aigu sévère; Temps réel; Détection; Réaction chaîne polymérase RT; Syndrome respiratoire aigu sévère</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Severe acute respiratory syndrome virus; Real time; Detection; Reverse transcription polymerase chain reaction; Severe acute respiratory syndrome</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Severe acute respiratory syndrome virus; Tiempo real; Detección; Reacción cadena polimerasa transcripción inversa; Síndrome respiratorio agudo severo</SD>
<LO>INIST-17422.354000131964810220</LO>
<ID>06-0194439</ID>
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