SrasV1, PascalFrancis, Corpus, bibRecord, 000524

Rapid and sensitive detection of multiple genes from the SARS-coronavirus using quantitative RT-PCR with dual systems

Identifieur interne : 000524 ( PascalFrancis/Corpus ); précédent : 000523; suivant : 000525

Rapid and sensitive detection of multiple genes from the SARS-coronavirus using quantitative RT-PCR with dual systems

Auteurs : Jau-Ling Huang ; Hui-Tsu Lin ; Yu-Ming Wang ; Yi-Chien Yeh ; Konan Peck ; Bai-Ling Lin ; Huan-Wun Liu ; Ann Chen ; Chang-Shen Lin

Source :

Journal of medical virology [ 0146-6615 ] ; 2005.

RBID : Pascal:06-0194441

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Détection, Gène, Analyse quantitative, Réaction chaîne polymérase RT, Temps réel, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Detection, Gene, Quantitative analysis, Real time, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.

Abstract

The outbreak of severe acute respiratory syndrome (SARS) was caused by a newly identified coronavirus (SARS-CoV) in 2003. To detect early SARS-CoV infection, a one-step, real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that could simultaneously detect nucleocapsid (N), membrane (M), and spike (S) genes of SARS-CoV with the same PCR condition using eitherApplied Biosystems (ABI) Prism 7700 Sequence Detection System or Roche LightCycler. The sensitivity of this assay was evaluated using cell culture-derived viruses, in vitro transcribed viral RNA, and clinical specimens. The SARS-S, -M, and -N primer/probe sets described in this paper could detect one to ten copies of in vitro transcribed S, M, and N RNA per test using both the ABI and Roche assay systems. The relative sensitivities for detecting cell culture-derived SARS-CoV were 0.01, 0.01, and 0.001 PFU/test, respectively. It showed that SARS-N has comparable detection efficiencies to SARS2 and SARS3 which are primers sets designed by Centers for Disease Control and Prevention. In addition, SARS-S and SARS-M also demonstrated equivalent sensitivity to the commercially available RealArt HPA-Coronavirus reagents (Artus). The relative sensitivity of these primer/probe sets was also examined using human sera spiked viruses and clinical specimens from four confirmed SARS patients. Similar results as above were obtained. Specificity tests and sequence alignment showed that these primer/probe sets annealed perfectly to 31 isolates of SARS-CoV; and there was no cross detection with other coronaviruses and human respiratory tract-associated viruses. Therefore, not only is it compatible with the ABI and Roche systems, this multiple-gene detection assay also has the merit of being a rapid, safe, sensitive, and specific tool for accurate diagnosis of SARS-CoV infection.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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Format Inist (serveur)

NO :	PASCAL 06-0194441 INIST
ET :	Rapid and sensitive detection of multiple genes from the SARS-coronavirus using quantitative RT-PCR with dual systems
AU :	HUANG (Jau-Ling); LIN (Hui-Tsu); WANG (Yu-Ming); YEH (Yi-Chien); PECK (Konan); LIN (Bai-Ling); LIU (Huan-Wun); CHEN (Ann); LIN (Chang-Shen)
AF :	Institute of Preventive Medicine, National Defense Medical Center/Taipei/Taïwan (1 aut., 2 aut., 3 aut., 4 aut., 7 aut., 8 aut.); Department of Bioscience Technology, College of Health Sciences, Chang Jung Christian University/Tainan/Taïwan (1 aut.); Institute of Medical Research, Chang Jung Christian University/Tainan/Taïwan (4 aut.); Institute of Biomedical Science, Academia Sinica/Taipei/Taïwan (5 aut.); Development Center for Biotechnology/Taipei/Taïwan (6 aut.); National Health Research Institutes/Miaoli/Taïwan (9 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 2; Pp. 151-158; Bibl. 31 ref.
LA :	Anglais
EA :	The outbreak of severe acute respiratory syndrome (SARS) was caused by a newly identified coronavirus (SARS-CoV) in 2003. To detect early SARS-CoV infection, a one-step, real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that could simultaneously detect nucleocapsid (N), membrane (M), and spike (S) genes of SARS-CoV with the same PCR condition using eitherApplied Biosystems (ABI) Prism 7700 Sequence Detection System or Roche LightCycler. The sensitivity of this assay was evaluated using cell culture-derived viruses, in vitro transcribed viral RNA, and clinical specimens. The SARS-S, -M, and -N primer/probe sets described in this paper could detect one to ten copies of in vitro transcribed S, M, and N RNA per test using both the ABI and Roche assay systems. The relative sensitivities for detecting cell culture-derived SARS-CoV were 0.01, 0.01, and 0.001 PFU/test, respectively. It showed that SARS-N has comparable detection efficiencies to SARS2 and SARS3 which are primers sets designed by Centers for Disease Control and Prevention. In addition, SARS-S and SARS-M also demonstrated equivalent sensitivity to the commercially available RealArt HPA-Coronavirus reagents (Artus). The relative sensitivity of these primer/probe sets was also examined using human sera spiked viruses and clinical specimens from four confirmed SARS patients. Similar results as above were obtained. Specificity tests and sequence alignment showed that these primer/probe sets annealed perfectly to 31 isolates of SARS-CoV; and there was no cross detection with other coronaviruses and human respiratory tract-associated viruses. Therefore, not only is it compatible with the ABI and Roche systems, this multiple-gene detection assay also has the merit of being a rapid, safe, sensitive, and specific tool for accurate diagnosis of SARS-CoV infection.
CC :	002A05C10; 002B05C02J
FD :	Virus syndrome respiratoire aigu sévère; Détection; Gène; Analyse quantitative; Réaction chaîne polymérase RT; Temps réel; Syndrome respiratoire aigu sévère
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Severe acute respiratory syndrome virus; Detection; Gene; Quantitative analysis; Reverse transcription polymerase chain reaction; Real time; Severe acute respiratory syndrome
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Severe acute respiratory syndrome virus; Detección; Gen; Análisis cuantitativo; Reacción cadena polimerasa transcripción inversa; Tiempo real; Síndrome respiratorio agudo severo
LO :	INIST-17422.354000132135750020
ID :	06-0194441

Links to Exploration step

Pascal:06-0194441

Le document en format XML

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<author><name sortKey="Lin, Chang Shen" sort="Lin, Chang Shen" uniqKey="Lin C" first="Chang-Shen" last="Lin">Chang-Shen Lin</name>
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<front><div type="abstract" xml:lang="en">The outbreak of severe acute respiratory syndrome (SARS) was caused by a newly identified coronavirus (SARS-CoV) in 2003. To detect early SARS-CoV infection, a one-step, real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that could simultaneously detect nucleocapsid (N), membrane (M), and spike (S) genes of SARS-CoV with the same PCR condition using eitherApplied Biosystems (ABI) Prism 7700 Sequence Detection System or Roche LightCycler. The sensitivity of this assay was evaluated using cell culture-derived viruses, in vitro transcribed viral RNA, and clinical specimens. The SARS-S, -M, and -N primer/probe sets described in this paper could detect one to ten copies of in vitro transcribed S, M, and N RNA per test using both the ABI and Roche assay systems. The relative sensitivities for detecting cell culture-derived SARS-CoV were 0.01, 0.01, and 0.001 PFU/test, respectively. It showed that SARS-N has comparable detection efficiencies to SARS2 and SARS3 which are primers sets designed by Centers for Disease Control and Prevention. In addition, SARS-S and SARS-M also demonstrated equivalent sensitivity to the commercially available RealArt HPA-Coronavirus reagents (Artus). The relative sensitivity of these primer/probe sets was also examined using human sera spiked viruses and clinical specimens from four confirmed SARS patients. Similar results as above were obtained. Specificity tests and sequence alignment showed that these primer/probe sets annealed perfectly to 31 isolates of SARS-CoV; and there was no cross detection with other coronaviruses and human respiratory tract-associated viruses. Therefore, not only is it compatible with the ABI and Roche systems, this multiple-gene detection assay also has the merit of being a rapid, safe, sensitive, and specific tool for accurate diagnosis of SARS-CoV infection.</div>
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<server><NO>PASCAL 06-0194441 INIST</NO>
<ET>Rapid and sensitive detection of multiple genes from the SARS-coronavirus using quantitative RT-PCR with dual systems</ET>
<AU>HUANG (Jau-Ling); LIN (Hui-Tsu); WANG (Yu-Ming); YEH (Yi-Chien); PECK (Konan); LIN (Bai-Ling); LIU (Huan-Wun); CHEN (Ann); LIN (Chang-Shen)</AU>
<AF>Institute of Preventive Medicine, National Defense Medical Center/Taipei/Taïwan (1 aut., 2 aut., 3 aut., 4 aut., 7 aut., 8 aut.); Department of Bioscience Technology, College of Health Sciences, Chang Jung Christian University/Tainan/Taïwan (1 aut.); Institute of Medical Research, Chang Jung Christian University/Tainan/Taïwan (4 aut.); Institute of Biomedical Science, Academia Sinica/Taipei/Taïwan (5 aut.); Development Center for Biotechnology/Taipei/Taïwan (6 aut.); National Health Research Institutes/Miaoli/Taïwan (9 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 2; Pp. 151-158; Bibl. 31 ref.</SO>
<LA>Anglais</LA>
<EA>The outbreak of severe acute respiratory syndrome (SARS) was caused by a newly identified coronavirus (SARS-CoV) in 2003. To detect early SARS-CoV infection, a one-step, real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that could simultaneously detect nucleocapsid (N), membrane (M), and spike (S) genes of SARS-CoV with the same PCR condition using eitherApplied Biosystems (ABI) Prism 7700 Sequence Detection System or Roche LightCycler. The sensitivity of this assay was evaluated using cell culture-derived viruses, in vitro transcribed viral RNA, and clinical specimens. The SARS-S, -M, and -N primer/probe sets described in this paper could detect one to ten copies of in vitro transcribed S, M, and N RNA per test using both the ABI and Roche assay systems. The relative sensitivities for detecting cell culture-derived SARS-CoV were 0.01, 0.01, and 0.001 PFU/test, respectively. It showed that SARS-N has comparable detection efficiencies to SARS2 and SARS3 which are primers sets designed by Centers for Disease Control and Prevention. In addition, SARS-S and SARS-M also demonstrated equivalent sensitivity to the commercially available RealArt HPA-Coronavirus reagents (Artus). The relative sensitivity of these primer/probe sets was also examined using human sera spiked viruses and clinical specimens from four confirmed SARS patients. Similar results as above were obtained. Specificity tests and sequence alignment showed that these primer/probe sets annealed perfectly to 31 isolates of SARS-CoV; and there was no cross detection with other coronaviruses and human respiratory tract-associated viruses. Therefore, not only is it compatible with the ABI and Roche systems, this multiple-gene detection assay also has the merit of being a rapid, safe, sensitive, and specific tool for accurate diagnosis of SARS-CoV infection.</EA>
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<ED>Severe acute respiratory syndrome virus; Detection; Gene; Quantitative analysis; Reverse transcription polymerase chain reaction; Real time; Severe acute respiratory syndrome</ED>
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Rapid and sensitive detection of multiple genes from the SARS-coronavirus using quantitative RT-PCR with dual systems

Rapid and sensitive detection of multiple genes from the SARS-coronavirus using quantitative RT-PCR with dual systems

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