SrasV1, PascalFrancis, Corpus, bibRecord, 000415

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Identifieur interne : 000415 ( PascalFrancis/Corpus ); précédent : 000414; suivant : 000416

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Auteurs : S. Escutenaire ; N. Mohamed ; M. Isaksson ; P. Thoren ; B. Klingeborn ; S. Belak ; M. Berg ; J. Blomberg

Source :

Archives of virology [ 0304-8608 ] ; 2007.

RBID : Pascal:07-0088920

Descripteurs français

Pascal (Inist)
- Temps réel, Réaction chaîne polymérase RT, Détection.

English descriptors

KwdEn :
- Detection, Real time, Reverse transcription polymerase chain reaction.

Abstract

Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0304-8608`
A03		`1`		`@0 Arch. virol.`
A05				`@2 152`
A06				`@2 1`
A08	`01`	`1`	`ENG`	`@1 SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses`
A11	`01`	`1`		`@1 ESCUTENAIRE (S.)`
A11	`02`	`1`		`@1 MOHAMED (N.)`
A11	`03`	`1`		`@1 ISAKSSON (M.)`
A11	`04`	`1`		`@1 THOREN (P.)`
A11	`05`	`1`		`@1 KLINGEBORN (B.)`
A11	`06`	`1`		`@1 BELAK (S.)`
A11	`07`	`1`		`@1 BERG (M.)`
A11	`08`	`1`		`@1 BLOMBERG (J.)`
A14	`01`			`@1 Department of Virology, National Veterinary Institute @2 Uppsala @3 SWE @Z 1 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut.`
A14	`02`			`@1 Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University @2 Uppsala @3 SWE @Z 2 aut. @Z 8 aut.`
A14	`03`			`@1 Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences @2 Uppsala @3 SWE @Z 6 aut. @Z 7 aut.`
A20				`@1 41-58`
A21				`@1 2007`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 6355 @5 354000159582010040`
A44				`@0 0000 @1 © 2007 INIST-CNRS. All rights reserved.`
A45				`@0 41 ref.`
A47	`01`	`1`		`@0 07-0088920`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Archives of virology`
A66	`01`			`@0 AUT`
C01	`01`		`ENG`	@0 Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.
C02	`01`	`X`		`@0 002A05C10`
C03	`01`	`X`	`FRE`	`@0 Temps réel @5 05`
C03	`01`	`X`	`ENG`	`@0 Real time @5 05`
C03	`01`	`X`	`SPA`	`@0 Tiempo real @5 05`
C03	`02`	`X`	`FRE`	`@0 Réaction chaîne polymérase RT @5 06`
C03	`02`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 06`
C03	`02`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 06`
C03	`03`	`X`	`FRE`	`@0 Détection @5 07`
C03	`03`	`X`	`ENG`	`@0 Detection @5 07`
C03	`03`	`X`	`SPA`	`@0 Detección @5 07`
N21				`@1 057`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 07-0088920 INIST
ET :	SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses
AU :	ESCUTENAIRE (S.); MOHAMED (N.); ISAKSSON (M.); THOREN (P.); KLINGEBORN (B.); BELAK (S.); BERG (M.); BLOMBERG (J.)
AF :	Department of Virology, National Veterinary Institute/Uppsala/Suède (1 aut., 3 aut., 4 aut., 5 aut., 6 aut.); Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University/Uppsala/Suède (2 aut., 8 aut.); Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences/Uppsala/Suède (6 aut., 7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Archives of virology; ISSN 0304-8608; Autriche; Da. 2007; Vol. 152; No. 1; Pp. 41-58; Bibl. 41 ref.
LA :	Anglais
EA :	Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.
CC :	002A05C10
FD :	Temps réel; Réaction chaîne polymérase RT; Détection
ED :	Real time; Reverse transcription polymerase chain reaction; Detection
SD :	Tiempo real; Reacción cadena polimerasa transcripción inversa; Detección
LO :	INIST-6355.354000159582010040
ID :	07-0088920

Links to Exploration step

Pascal:07-0088920

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses</title>
<author><name sortKey="Escutenaire, S" sort="Escutenaire, S" uniqKey="Escutenaire S" first="S." last="Escutenaire">S. Escutenaire</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Mohamed, N" sort="Mohamed, N" uniqKey="Mohamed N" first="N." last="Mohamed">N. Mohamed</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Isaksson, M" sort="Isaksson, M" uniqKey="Isaksson M" first="M." last="Isaksson">M. Isaksson</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Thoren, P" sort="Thoren, P" uniqKey="Thoren P" first="P." last="Thoren">P. Thoren</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Klingeborn, B" sort="Klingeborn, B" uniqKey="Klingeborn B" first="B." last="Klingeborn">B. Klingeborn</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Belak, S" sort="Belak, S" uniqKey="Belak S" first="S." last="Belak">S. Belak</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Berg, M" sort="Berg, M" uniqKey="Berg M" first="M." last="Berg">M. Berg</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Blomberg, J" sort="Blomberg, J" uniqKey="Blomberg J" first="J." last="Blomberg">J. Blomberg</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0088920</idno>
<date when="2007">2007</date>
<idno type="stanalyst">PASCAL 07-0088920 INIST</idno>
<idno type="RBID">Pascal:07-0088920</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000415</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses</title>
<author><name sortKey="Escutenaire, S" sort="Escutenaire, S" uniqKey="Escutenaire S" first="S." last="Escutenaire">S. Escutenaire</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Mohamed, N" sort="Mohamed, N" uniqKey="Mohamed N" first="N." last="Mohamed">N. Mohamed</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Isaksson, M" sort="Isaksson, M" uniqKey="Isaksson M" first="M." last="Isaksson">M. Isaksson</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Thoren, P" sort="Thoren, P" uniqKey="Thoren P" first="P." last="Thoren">P. Thoren</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Klingeborn, B" sort="Klingeborn, B" uniqKey="Klingeborn B" first="B." last="Klingeborn">B. Klingeborn</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Belak, S" sort="Belak, S" uniqKey="Belak S" first="S." last="Belak">S. Belak</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Berg, M" sort="Berg, M" uniqKey="Berg M" first="M." last="Berg">M. Berg</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Blomberg, J" sort="Blomberg, J" uniqKey="Blomberg J" first="J." last="Blomberg">J. Blomberg</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term>
<term>Real time</term>
<term>Reverse transcription polymerase chain reaction</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Temps réel</term>
<term>Réaction chaîne polymérase RT</term>
<term>Détection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0304-8608</s0>
</fA01>
<fA03 i2="1"><s0>Arch. virol.</s0>
</fA03>
<fA05><s2>152</s2>
</fA05>
<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>ESCUTENAIRE (S.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>MOHAMED (N.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>ISAKSSON (M.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>THOREN (P.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>KLINGEBORN (B.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BELAK (S.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>BERG (M.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>BLOMBERG (J.)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>41-58</s1>
</fA20>
<fA21><s1>2007</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>6355</s2>
<s5>354000159582010040</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2007 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>41 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>07-0088920</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Archives of virology</s0>
</fA64>
<fA66 i1="01"><s0>AUT</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Temps réel</s0>
<s5>05</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Real time</s0>
<s5>05</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Tiempo real</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Réaction chaîne polymérase RT</s0>
<s5>06</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Reverse transcription polymerase chain reaction</s0>
<s5>06</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Reacción cadena polimerasa transcripción inversa</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Détection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Detection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Detección</s0>
<s5>07</s5>
</fC03>
<fN21><s1>057</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 07-0088920 INIST</NO>
<ET>SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses</ET>
<AU>ESCUTENAIRE (S.); MOHAMED (N.); ISAKSSON (M.); THOREN (P.); KLINGEBORN (B.); BELAK (S.); BERG (M.); BLOMBERG (J.)</AU>
<AF>Department of Virology, National Veterinary Institute/Uppsala/Suède (1 aut., 3 aut., 4 aut., 5 aut., 6 aut.); Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University/Uppsala/Suède (2 aut., 8 aut.); Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences/Uppsala/Suède (6 aut., 7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Archives of virology; ISSN 0304-8608; Autriche; Da. 2007; Vol. 152; No. 1; Pp. 41-58; Bibl. 41 ref.</SO>
<LA>Anglais</LA>
<EA>Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.</EA>
<CC>002A05C10</CC>
<FD>Temps réel; Réaction chaîne polymérase RT; Détection</FD>
<ED>Real time; Reverse transcription polymerase chain reaction; Detection</ED>
<SD>Tiempo real; Reacción cadena polimerasa transcripción inversa; Detección</SD>
<LO>INIST-6355.354000159582010040</LO>
<ID>07-0088920</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000415 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000415 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:07-0088920
   |texte=   SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Source :

Descripteurs français

English descriptors

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri