Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development
Identifieur interne : 000202 ( PascalFrancis/Corpus ); précédent : 000201; suivant : 000203Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development
Auteurs : HONGYUAN SHEN ; SHOU GUO FANG ; BO CHEN ; GUANG CHEN ; Felicia P. L. Tay ; DING XIANG LIUSource :
- Journal of virological methods [ 0166-0934 ] ; 2009.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
|
---|
Format Inist (serveur)
NO : | PASCAL 09-0319418 INIST |
---|---|
ET : | Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development |
AU : | HONGYUAN SHEN; SHOU GUO FANG; BO CHEN; GUANG CHEN; TAY (Felicia P. L.); DING XIANG LIU |
AF : | Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos/Singapore 138673/Singapour (1 aut., 2 aut., 5 aut., 6 aut.); School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive/Singapore 637551/Singapour (3 aut., 6 aut.); Department of Biological Sciences, National University of Singapore/Singapore 117543/Singapour (4 aut., 6 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2009; Vol. 160; No. 1-2; Pp. 48-56; Bibl. 1 p.1/4 |
LA : | Anglais |
EA : | Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken. |
CC : | 002A05C09 |
FD : | Virus bronchite infectieuse aviaire; Vecteur; Gène; Vaccin; Génétique; Microbiologie; Méthode; Virologie; Bronchite infectieuse aviaire |
FG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Vétérinaire; Virose; Infection |
ED : | Avian infectious bronchitis virus; Vector; Gene; Vaccine; Genetics; Microbiology; Method; Virology; Avian infectious bronchitis |
EG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Veterinary; Viral disease; Infection |
SD : | Avian infectious bronchitis virus; Vector; Gen; Vacuna; Genética; Microbiología; Método; Virología; Bronquitis infecciosa aviar |
LO : | INIST-18295.354000187985960070 |
ID : | 09-0319418 |
Links to Exploration step
Pascal:09-0319418Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development</title>
<author><name sortKey="Hongyuan Shen" sort="Hongyuan Shen" uniqKey="Hongyuan Shen" last="Hongyuan Shen">HONGYUAN SHEN</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Shou Guo Fang" sort="Shou Guo Fang" uniqKey="Shou Guo Fang" last="Shou Guo Fang">SHOU GUO FANG</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Bo Chen" sort="Bo Chen" uniqKey="Bo Chen" last="Bo Chen">BO CHEN</name>
<affiliation><inist:fA14 i1="02"><s1>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive</s1>
<s2>Singapore 637551</s2>
<s3>SGP</s3>
<sZ>3 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Guang Chen" sort="Guang Chen" uniqKey="Guang Chen" last="Guang Chen">GUANG CHEN</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Biological Sciences, National University of Singapore</s1>
<s2>Singapore 117543</s2>
<s3>SGP</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P. L." last="Tay">Felicia P. L. Tay</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ding Xiang Liu" sort="Ding Xiang Liu" uniqKey="Ding Xiang Liu" last="Ding Xiang Liu">DING XIANG LIU</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive</s1>
<s2>Singapore 637551</s2>
<s3>SGP</s3>
<sZ>3 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Biological Sciences, National University of Singapore</s1>
<s2>Singapore 117543</s2>
<s3>SGP</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">09-0319418</idno>
<date when="2009">2009</date>
<idno type="stanalyst">PASCAL 09-0319418 INIST</idno>
<idno type="RBID">Pascal:09-0319418</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000202</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development</title>
<author><name sortKey="Hongyuan Shen" sort="Hongyuan Shen" uniqKey="Hongyuan Shen" last="Hongyuan Shen">HONGYUAN SHEN</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Shou Guo Fang" sort="Shou Guo Fang" uniqKey="Shou Guo Fang" last="Shou Guo Fang">SHOU GUO FANG</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Bo Chen" sort="Bo Chen" uniqKey="Bo Chen" last="Bo Chen">BO CHEN</name>
<affiliation><inist:fA14 i1="02"><s1>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive</s1>
<s2>Singapore 637551</s2>
<s3>SGP</s3>
<sZ>3 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Guang Chen" sort="Guang Chen" uniqKey="Guang Chen" last="Guang Chen">GUANG CHEN</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Biological Sciences, National University of Singapore</s1>
<s2>Singapore 117543</s2>
<s3>SGP</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P. L." last="Tay">Felicia P. L. Tay</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ding Xiang Liu" sort="Ding Xiang Liu" uniqKey="Ding Xiang Liu" last="Ding Xiang Liu">DING XIANG LIU</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive</s1>
<s2>Singapore 637551</s2>
<s3>SGP</s3>
<sZ>3 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Biological Sciences, National University of Singapore</s1>
<s2>Singapore 117543</s2>
<s3>SGP</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of virological methods</title>
<title level="j" type="abbreviated">J. virol. methods</title>
<idno type="ISSN">0166-0934</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virological methods</title>
<title level="j" type="abbreviated">J. virol. methods</title>
<idno type="ISSN">0166-0934</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Avian infectious bronchitis</term>
<term>Avian infectious bronchitis virus</term>
<term>Gene</term>
<term>Genetics</term>
<term>Method</term>
<term>Microbiology</term>
<term>Vaccine</term>
<term>Vector</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus bronchite infectieuse aviaire</term>
<term>Vecteur</term>
<term>Gène</term>
<term>Vaccin</term>
<term>Génétique</term>
<term>Microbiologie</term>
<term>Méthode</term>
<term>Virologie</term>
<term>Bronchite infectieuse aviaire</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-0934</s0>
</fA01>
<fA02 i1="01"><s0>JVMEDH</s0>
</fA02>
<fA03 i2="1"><s0>J. virol. methods</s0>
</fA03>
<fA05><s2>160</s2>
</fA05>
<fA06><s2>1-2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>HONGYUAN SHEN</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>SHOU GUO FANG</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>BO CHEN</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>GUANG CHEN</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>TAY (Felicia P. L.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>DING XIANG LIU</s1>
</fA11>
<fA14 i1="01"><s1>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos</s1>
<s2>Singapore 138673</s2>
<s3>SGP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive</s1>
<s2>Singapore 637551</s2>
<s3>SGP</s3>
<sZ>3 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Biological Sciences, National University of Singapore</s1>
<s2>Singapore 117543</s2>
<s3>SGP</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA20><s1>48-56</s1>
</fA20>
<fA21><s1>2009</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18295</s2>
<s5>354000187985960070</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2009 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1 p.1/4</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>09-0319418</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virological methods</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C09</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Virus bronchite infectieuse aviaire</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Avian infectious bronchitis virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Avian infectious bronchitis virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Vecteur</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Vector</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Vector</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Gène</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Gene</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Gen</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Vaccin</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Vaccine</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Vacuna</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Génétique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Genetics</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Genética</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Méthode</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Method</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Método</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Virologie</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Virology</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Virología</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Bronchite infectieuse aviaire</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Avian infectious bronchitis</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Bronquitis infecciosa aviar</s0>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Vétérinaire</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Veterinary</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Veterinario</s0>
<s5>13</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fN21><s1>229</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 09-0319418 INIST</NO>
<ET>Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development</ET>
<AU>HONGYUAN SHEN; SHOU GUO FANG; BO CHEN; GUANG CHEN; TAY (Felicia P. L.); DING XIANG LIU</AU>
<AF>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos/Singapore 138673/Singapour (1 aut., 2 aut., 5 aut., 6 aut.); School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive/Singapore 637551/Singapour (3 aut., 6 aut.); Department of Biological Sciences, National University of Singapore/Singapore 117543/Singapour (4 aut., 6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2009; Vol. 160; No. 1-2; Pp. 48-56; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</EA>
<CC>002A05C09</CC>
<FD>Virus bronchite infectieuse aviaire; Vecteur; Gène; Vaccin; Génétique; Microbiologie; Méthode; Virologie; Bronchite infectieuse aviaire</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Vétérinaire; Virose; Infection</FG>
<ED>Avian infectious bronchitis virus; Vector; Gene; Vaccine; Genetics; Microbiology; Method; Virology; Avian infectious bronchitis</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Veterinary; Viral disease; Infection</EG>
<SD>Avian infectious bronchitis virus; Vector; Gen; Vacuna; Genética; Microbiología; Método; Virología; Bronquitis infecciosa aviar</SD>
<LO>INIST-18295.354000187985960070</LO>
<ID>09-0319418</ID>
</server>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000202 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000202 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PascalFrancis |étape= Corpus |type= RBID |clé= Pascal:09-0319418 |texte= Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |