SrasV1, PascalFrancis, Corpus, bibRecord, 000202

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Identifieur interne : 000202 ( PascalFrancis/Corpus ); précédent : 000201; suivant : 000203

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Auteurs : HONGYUAN SHEN ; SHOU GUO FANG ; BO CHEN ; GUANG CHEN ; Felicia P. L. Tay ; DING XIANG LIU

Source :

Journal of virological methods [ 0166-0934 ] ; 2009.

RBID : Pascal:09-0319418

Descripteurs français

Pascal (Inist)
- Virus bronchite infectieuse aviaire, Vecteur, Gène, Vaccin, Génétique, Microbiologie, Méthode, Virologie, Bronchite infectieuse aviaire.

English descriptors

KwdEn :
- Avian infectious bronchitis, Avian infectious bronchitis virus, Gene, Genetics, Method, Microbiology, Vaccine, Vector, Virology.

Abstract

Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 09-0319418 INIST
ET :	Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development
AU :	HONGYUAN SHEN; SHOU GUO FANG; BO CHEN; GUANG CHEN; TAY (Felicia P. L.); DING XIANG LIU
AF :	Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos/Singapore 138673/Singapour (1 aut., 2 aut., 5 aut., 6 aut.); School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive/Singapore 637551/Singapour (3 aut., 6 aut.); Department of Biological Sciences, National University of Singapore/Singapore 117543/Singapour (4 aut., 6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2009; Vol. 160; No. 1-2; Pp. 48-56; Bibl. 1 p.1/4
LA :	Anglais
EA :	Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.
CC :	002A05C09
FD :	Virus bronchite infectieuse aviaire; Vecteur; Gène; Vaccin; Génétique; Microbiologie; Méthode; Virologie; Bronchite infectieuse aviaire
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Vétérinaire; Virose; Infection
ED :	Avian infectious bronchitis virus; Vector; Gene; Vaccine; Genetics; Microbiology; Method; Virology; Avian infectious bronchitis
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Veterinary; Viral disease; Infection
SD :	Avian infectious bronchitis virus; Vector; Gen; Vacuna; Genética; Microbiología; Método; Virología; Bronquitis infecciosa aviar
LO :	INIST-18295.354000187985960070
ID :	09-0319418

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Pascal:09-0319418

Le document en format XML

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<front><div type="abstract" xml:lang="en">Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</div>
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<fA11 i1="01" i2="1"><s1>HONGYUAN SHEN</s1>
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<fA14 i1="03"><s1>Department of Biological Sciences, National University of Singapore</s1>
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<server><NO>PASCAL 09-0319418 INIST</NO>
<ET>Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development</ET>
<AU>HONGYUAN SHEN; SHOU GUO FANG; BO CHEN; GUANG CHEN; TAY (Felicia P. L.); DING XIANG LIU</AU>
<AF>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos/Singapore 138673/Singapour (1 aut., 2 aut., 5 aut., 6 aut.); School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive/Singapore 637551/Singapour (3 aut., 6 aut.); Department of Biological Sciences, National University of Singapore/Singapore 117543/Singapour (4 aut., 6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virological methods; ISSN 0166-0934; Coden JVMEDH; Pays-Bas; Da. 2009; Vol. 160; No. 1-2; Pp. 48-56; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</EA>
<CC>002A05C09</CC>
<FD>Virus bronchite infectieuse aviaire; Vecteur; Gène; Vaccin; Génétique; Microbiologie; Méthode; Virologie; Bronchite infectieuse aviaire</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Vétérinaire; Virose; Infection</FG>
<ED>Avian infectious bronchitis virus; Vector; Gene; Vaccine; Genetics; Microbiology; Method; Virology; Avian infectious bronchitis</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Veterinary; Viral disease; Infection</EG>
<SD>Avian infectious bronchitis virus; Vector; Gen; Vacuna; Genética; Microbiología; Método; Virología; Bronquitis infecciosa aviar</SD>
<LO>INIST-18295.354000187985960070</LO>
<ID>09-0319418</ID>
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Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development

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