Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells
Identifieur interne : 000824 ( PascalFrancis/Checkpoint ); précédent : 000823; suivant : 000825Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells
Auteurs : ZHI WANG [République populaire de Chine] ; Lili Ren [République populaire de Chine] ; XINGANG ZHAO [République populaire de Chine] ; TAO HUNG [République populaire de Chine] ; ANMING MENG [République populaire de Chine] ; JIANWEI WANG [République populaire de Chine] ; YE-GUANG CHEN [République populaire de Chine]Source :
- Journal of virology [ 0022-538X ] ; 2004.
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Abstract
Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells</title><author><name sortKey="Zhi Wang" sort="Zhi Wang" uniqKey="Zhi Wang" last="Zhi Wang">ZHI WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xingang Zhao" sort="Xingang Zhao" uniqKey="Xingang Zhao" last="Xingang Zhao">XINGANG ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Tao Hung" sort="Tao Hung" uniqKey="Tao Hung" last="Tao Hung">TAO HUNG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Anming Meng" sort="Anming Meng" uniqKey="Anming Meng" last="Anming Meng">ANMING MENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jianwei Wang" sort="Jianwei Wang" uniqKey="Jianwei Wang" last="Jianwei Wang">JIANWEI WANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ye Guang Chen" sort="Ye Guang Chen" uniqKey="Ye Guang Chen" last="Ye-Guang Chen">YE-GUANG CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0393340</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0393340 INIST</idno><idno type="RBID">Pascal:04-0393340</idno><idno type="wicri:Area/PascalFrancis/Corpus">000845</idno><idno type="wicri:Area/PascalFrancis/Curation">000145</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000824</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000824</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells</title><author><name sortKey="Zhi Wang" sort="Zhi Wang" uniqKey="Zhi Wang" last="Zhi Wang">ZHI WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xingang Zhao" sort="Xingang Zhao" uniqKey="Xingang Zhao" last="Xingang Zhao">XINGANG ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Tao Hung" sort="Tao Hung" uniqKey="Tao Hung" last="Tao Hung">TAO HUNG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Anming Meng" sort="Anming Meng" uniqKey="Anming Meng" last="Anming Meng">ANMING MENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jianwei Wang" sort="Jianwei Wang" uniqKey="Jianwei Wang" last="Jianwei Wang">JIANWEI WANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ye Guang Chen" sort="Ye Guang Chen" uniqKey="Ye Guang Chen" last="Ye-Guang Chen">YE-GUANG CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene silencing</term><term>Mammalia</term><term>Microbiology</term><term>Replication</term><term>Severe acute respiratory syndrome</term><term>Virology</term><term>Virus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus</term><term>Mammalia</term><term>Réplication</term><term>Silence expression génique</term><term>Microbiologie</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0></fA01><fA03 i2="1"><s0>J. virol.</s0></fA03><fA05><s2>78</s2></fA05><fA06><s2>14</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells</s1></fA08><fA11 i1="01" i2="1"><s1>ZHI WANG</s1></fA11><fA11 i1="02" i2="1"><s1>REN (Lili)</s1></fA11><fA11 i1="03" i2="1"><s1>XINGANG ZHAO</s1></fA11><fA11 i1="04" i2="1"><s1>TAO HUNG</s1></fA11><fA11 i1="05" i2="1"><s1>ANMING MENG</s1></fA11><fA11 i1="06" i2="1"><s1>JIANWEI WANG</s1></fA11><fA11 i1="07" i2="1"><s1>YE-GUANG CHEN</s1></fA11><fA14 i1="01"><s1>State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University</s1><s2>Beijing 100084</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="02"><s1>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention</s1><s2>Beijing 100052</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></fA14><fA20><s1>7523-7527</s1></fA20><fA21><s1>2004</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>13592</s2><s5>354000113683220250</s5></fA43><fA44><s0>0000</s0><s1>© 2004 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>32 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>04-0393340</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virology</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C10</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Mammalia</s0><s2>NS</s2><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Mammalia</s0><s2>NS</s2><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Mammalia</s0><s2>NS</s2><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Réplication</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Replication</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Replicación</s0><s5>05</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Silence expression génique</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Gene silencing</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Silencio expresión genética</s0><s5>06</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Microbiologie</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Microbiology</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Microbiología</s0><s5>07</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Virologie</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Virology</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Virología</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>14</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>14</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>14</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Virose</s0><s2>NM</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Viral disease</s0><s2>NM</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Virosis</s0><s2>NM</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Infection</s0><s2>NM</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Infection</s0><s2>NM</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Infección</s0><s2>NM</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>19</s5></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>19</s5></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>19</s5></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>20</s5></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Lung disease</s0><s5>20</s5></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>20</s5></fC07><fN21><s1>222</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhi Wang" sort="Zhi Wang" uniqKey="Zhi Wang" last="Zhi Wang">ZHI WANG</name></noRegion><name sortKey="Anming Meng" sort="Anming Meng" uniqKey="Anming Meng" last="Anming Meng">ANMING MENG</name><name sortKey="Jianwei Wang" sort="Jianwei Wang" uniqKey="Jianwei Wang" last="Jianwei Wang">JIANWEI WANG</name><name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name><name sortKey="Tao Hung" sort="Tao Hung" uniqKey="Tao Hung" last="Tao Hung">TAO HUNG</name><name sortKey="Xingang Zhao" sort="Xingang Zhao" uniqKey="Xingang Zhao" last="Xingang Zhao">XINGANG ZHAO</name><name sortKey="Ye Guang Chen" sort="Ye Guang Chen" uniqKey="Ye Guang Chen" last="Ye-Guang Chen">YE-GUANG CHEN</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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