SrasV1, PascalFrancis, Checkpoint, bibRecord, 000367

Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice

Identifieur interne : 000367 ( PascalFrancis/Checkpoint ); précédent : 000366; suivant : 000368

Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice

Auteurs : Mary C. Hu [États-Unis] ; Taff Jones [Canada] ; Richard T. Kenney [États-Unis] ; Dale L. Barnard [États-Unis] ; David S. Burt [Canada] ; George H. Lowell [Canada]

Source :

Vaccine [ 0264-410X ] ; 2007.

RBID : Pascal:07-0388604

Descripteurs français

Pascal (Inist)
- Souris, Voie intranasale, Protéine recombinante, Protéine sérique, Anticorps neutralisant, Vaccin.
Wicri :
- topic : Vaccin.

English descriptors

KwdEn :
- Intranasal administration, Mouse, Neutralizing antibody, Recombinant protein, Serum protein, Vaccine.

Abstract

The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formulated S-protein vaccine elicited high levels of antigen-specific IgG in serum, comparable to those elicited by an intramuscular Alum-adsorbed S-protein vaccine. Serum antibodies were shown to be virus neutralizing. Intranasal immunization of young mice with the Protollin-formulated vaccine elicited significant levels of antigen-specific lung IgA in contrast to mice immunized with the intramuscular vaccine in which no antigen-specific lung IgA was detected. Following live virus challenge of aged mice, no virus was detected in the lungs of intranasally immunized mice, in contrast to intramuscularly immunized mice whose lung virus titers were comparable to those observed in control mice.

Affiliations:

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Pascal:07-0388604

Le document en format XML

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<author><name sortKey="Hu, Mary C" sort="Hu, Mary C" uniqKey="Hu M" first="Mary C." last="Hu">Mary C. Hu</name>
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<s2>Bothell, WA 98011</s2>
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<country>États-Unis</country>
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<author><name sortKey="Jones, Taff" sort="Jones, Taff" uniqKey="Jones T" first="Taff" last="Jones">Taff Jones</name>
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<s2>Montreal, Quebec H7V 3S8</s2>
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<country>Canada</country>
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<author><name sortKey="Kenney, Richard T" sort="Kenney, Richard T" uniqKey="Kenney R" first="Richard T." last="Kenney">Richard T. Kenney</name>
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<s2>Columbia, MA 21046</s2>
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<country>États-Unis</country>
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<author><name sortKey="Barnard, Dale L" sort="Barnard, Dale L" uniqKey="Barnard D" first="Dale L." last="Barnard">Dale L. Barnard</name>
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<author><name sortKey="Burt, David S" sort="Burt, David S" uniqKey="Burt D" first="David S." last="Burt">David S. Burt</name>
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<author><name sortKey="Lowell, George H" sort="Lowell, George H" uniqKey="Lowell G" first="George H." last="Lowell">George H. Lowell</name>
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<s2>Montreal, Quebec H7V 3S8</s2>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice</title>
<author><name sortKey="Hu, Mary C" sort="Hu, Mary C" uniqKey="Hu M" first="Mary C." last="Hu">Mary C. Hu</name>
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<s2>Bothell, WA 98011</s2>
<s3>USA</s3>
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<author><name sortKey="Jones, Taff" sort="Jones, Taff" uniqKey="Jones T" first="Taff" last="Jones">Taff Jones</name>
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<s2>Montreal, Quebec H7V 3S8</s2>
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<country>Canada</country>
<wicri:noRegion>Montreal, Quebec H7V 3S8</wicri:noRegion>
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</author>
<author><name sortKey="Kenney, Richard T" sort="Kenney, Richard T" uniqKey="Kenney R" first="Richard T." last="Kenney">Richard T. Kenney</name>
<affiliation wicri:level="2"><inist:fA14 i1="03"><s1>GlaxoSmithKline Biologicals North America of Maryland, 6996 Columbia Gateway Drive</s1>
<s2>Columbia, MA 21046</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Barnard, Dale L" sort="Barnard, Dale L" uniqKey="Barnard D" first="Dale L." last="Barnard">Dale L. Barnard</name>
<affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Institute for Antiviral Research, Department of ADVS, Utah State University</s1>
<s2>Logan, UT 84322</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
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<placeName><region type="state">Utah</region>
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<author><name sortKey="Burt, David S" sort="Burt, David S" uniqKey="Burt D" first="David S." last="Burt">David S. Burt</name>
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<s2>Montreal, Quebec H7V 3S8</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Montreal, Quebec H7V 3S8</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lowell, George H" sort="Lowell, George H" uniqKey="Lowell G" first="George H." last="Lowell">George H. Lowell</name>
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<s2>Montreal, Quebec H7V 3S8</s2>
<s3>CAN</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Canada</country>
<wicri:noRegion>Montreal, Quebec H7V 3S8</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Intranasal administration</term>
<term>Mouse</term>
<term>Neutralizing antibody</term>
<term>Recombinant protein</term>
<term>Serum protein</term>
<term>Vaccine</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Souris</term>
<term>Voie intranasale</term>
<term>Protéine recombinante</term>
<term>Protéine sérique</term>
<term>Anticorps neutralisant</term>
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</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term>
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<front><div type="abstract" xml:lang="en">The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formulated S-protein vaccine elicited high levels of antigen-specific IgG in serum, comparable to those elicited by an intramuscular Alum-adsorbed S-protein vaccine. Serum antibodies were shown to be virus neutralizing. Intranasal immunization of young mice with the Protollin-formulated vaccine elicited significant levels of antigen-specific lung IgA in contrast to mice immunized with the intramuscular vaccine in which no antigen-specific lung IgA was detected. Following live virus challenge of aged mice, no virus was detected in the lungs of intranasally immunized mice, in contrast to intramuscularly immunized mice whose lung virus titers were comparable to those observed in control mice.</div>
</front>
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<country name="Canada"><noRegion><name sortKey="Jones, Taff" sort="Jones, Taff" uniqKey="Jones T" first="Taff" last="Jones">Taff Jones</name>
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Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice

Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice

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Descripteurs français

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Abstract

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