Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice
Identifieur interne : 000356 ( PascalFrancis/Corpus ); précédent : 000355; suivant : 000357Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice
Auteurs : Mary C. Hu ; Taff Jones ; Richard T. Kenney ; Dale L. Barnard ; David S. Burt ; George H. LowellSource :
- Vaccine [ 0264-410X ] ; 2007.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formulated S-protein vaccine elicited high levels of antigen-specific IgG in serum, comparable to those elicited by an intramuscular Alum-adsorbed S-protein vaccine. Serum antibodies were shown to be virus neutralizing. Intranasal immunization of young mice with the Protollin-formulated vaccine elicited significant levels of antigen-specific lung IgA in contrast to mice immunized with the intramuscular vaccine in which no antigen-specific lung IgA was detected. Following live virus challenge of aged mice, no virus was detected in the lungs of intranasally immunized mice, in contrast to intramuscularly immunized mice whose lung virus titers were comparable to those observed in control mice.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 07-0388604 INIST |
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ET : | Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice |
AU : | HU (Mary C.); JONES (Taff); KENNEY (Richard T.); BARNARD (Dale L.); BURT (David S.); LOWELL (George H.) |
AF : | GlaxoSmithKline Biologicals North America of Washington, 19204 North Creek Parkway/Bothell, WA 98011/Etats-Unis (1 aut.); GlaxoSmithKline Biologicals North America of Quebec, 525 Cartier Blvd. West, Laval/Montreal, Quebec H7V 3S8/Canada (2 aut., 5 aut., 6 aut.); GlaxoSmithKline Biologicals North America of Maryland, 6996 Columbia Gateway Drive/Columbia, MA 21046/Etats-Unis (3 aut.); Institute for Antiviral Research, Department of ADVS, Utah State University/Logan, UT 84322/Etats-Unis (4 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Vaccine; ISSN 0264-410X; Coden VACCDE; Royaume-Uni; Da. 2007; Vol. 25; No. 34; Pp. 6334-6340; Bibl. 22 ref. |
LA : | Anglais |
EA : | The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formulated S-protein vaccine elicited high levels of antigen-specific IgG in serum, comparable to those elicited by an intramuscular Alum-adsorbed S-protein vaccine. Serum antibodies were shown to be virus neutralizing. Intranasal immunization of young mice with the Protollin-formulated vaccine elicited significant levels of antigen-specific lung IgA in contrast to mice immunized with the intramuscular vaccine in which no antigen-specific lung IgA was detected. Following live virus challenge of aged mice, no virus was detected in the lungs of intranasally immunized mice, in contrast to intramuscularly immunized mice whose lung virus titers were comparable to those observed in control mice. |
CC : | 002A05F04 |
FD : | Souris; Voie intranasale; Protéine recombinante; Protéine sérique; Anticorps neutralisant; Vaccin |
FG : | Rodentia; Mammalia; Vertebrata |
ED : | Mouse; Intranasal administration; Recombinant protein; Serum protein; Neutralizing antibody; Vaccine |
EG : | Rodentia; Mammalia; Vertebrata |
SD : | Ratón; Vía intranasal; Proteína recombinante; Proteína sérica; anticuerpo neutralizante; Vacuna |
LO : | INIST-20289.354000146734700080 |
ID : | 07-0388604 |
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Pascal:07-0388604Le document en format XML
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<front><div type="abstract" xml:lang="en">The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formulated S-protein vaccine elicited high levels of antigen-specific IgG in serum, comparable to those elicited by an intramuscular Alum-adsorbed S-protein vaccine. Serum antibodies were shown to be virus neutralizing. Intranasal immunization of young mice with the Protollin-formulated vaccine elicited significant levels of antigen-specific lung IgA in contrast to mice immunized with the intramuscular vaccine in which no antigen-specific lung IgA was detected. Following live virus challenge of aged mice, no virus was detected in the lungs of intranasally immunized mice, in contrast to intramuscularly immunized mice whose lung virus titers were comparable to those observed in control mice.</div>
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<server><NO>PASCAL 07-0388604 INIST</NO>
<ET>Intranasal Protollin-formulated recombinant SARS S-protein elicits respiratory and serum neutralizing antibodies and protection in mice</ET>
<AU>HU (Mary C.); JONES (Taff); KENNEY (Richard T.); BARNARD (Dale L.); BURT (David S.); LOWELL (George H.)</AU>
<AF>GlaxoSmithKline Biologicals North America of Washington, 19204 North Creek Parkway/Bothell, WA 98011/Etats-Unis (1 aut.); GlaxoSmithKline Biologicals North America of Quebec, 525 Cartier Blvd. West, Laval/Montreal, Quebec H7V 3S8/Canada (2 aut., 5 aut., 6 aut.); GlaxoSmithKline Biologicals North America of Maryland, 6996 Columbia Gateway Drive/Columbia, MA 21046/Etats-Unis (3 aut.); Institute for Antiviral Research, Department of ADVS, Utah State University/Logan, UT 84322/Etats-Unis (4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
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<LA>Anglais</LA>
<EA>The feasibility of developing a prophylactic vaccine against SARS was assessed by comparing the immune responses elicited by immunizing mice with a recombinant SARS spike glycoprotein (S-protein) formulated with different adjuvants, given by different routes. In both young and aged mice, an intranasal Protollin-formulated S-protein vaccine elicited high levels of antigen-specific IgG in serum, comparable to those elicited by an intramuscular Alum-adsorbed S-protein vaccine. Serum antibodies were shown to be virus neutralizing. Intranasal immunization of young mice with the Protollin-formulated vaccine elicited significant levels of antigen-specific lung IgA in contrast to mice immunized with the intramuscular vaccine in which no antigen-specific lung IgA was detected. Following live virus challenge of aged mice, no virus was detected in the lungs of intranasally immunized mice, in contrast to intramuscularly immunized mice whose lung virus titers were comparable to those observed in control mice.</EA>
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