Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus
Identifieur interne : 000361 ( PascalFrancis/Checkpoint ); précédent : 000360; suivant : 000362Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus
Auteurs : LI LIU [États-Unis] ; QING FANG [États-Unis] ; FEI DENG [République populaire de Chine] ; HANZHONG WANG [République populaire de Chine] ; Christopher E. Yi [États-Unis] ; LEI BA [États-Unis] ; WENJIE YU [États-Unis] ; Richard D. Lin [États-Unis] ; TAISHENG LI [République populaire de Chine] ; ZHIHONG HU [République populaire de Chine] ; David D. Ho [États-Unis] ; LINQI ZHANG [États-Unis, République populaire de Chine] ; ZHIWEI CHEN [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2007.
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Abstract
The severe acute respiratory syndrome (SARS) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. Coronavirus (CoV) found in naturally infected palm civet (civet-CoV) represents the closest genetic relative to SARS-CoV, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. Studies indicate that the receptor binding domain (RBD) of the SARS-CoV spike (S) glycoprotein contains major determinants for viral entry and neutralization. We aim to characterize the impact of natural mutations within the RBDs of civet-CoVs on viral entry and cross-neutralization. In this study, the S glycoprotein genes were recovered from naturally infected civets in central China (Hubei province), extending the geographic distribution of civet-CoV beyond the southeastern province of Guangdong. Moreover, pseudoviruses generated in our laboratory with four civet S genes, each with a distinct RBD, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of SARS-CoV. These four civet S genes were also constructed as DNA vaccines to immunize mice. Immunized sera elicited against most civet S glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing SARS-CoV and vice versa. Convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. Our findings suggest that the design of SARS vaccine should consider not only preventing the reemergence of SARS-CoV but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet CoVs of broad geographic origin.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus</title><author><name sortKey="Li Liu" sort="Li Liu" uniqKey="Li Liu" last="Li Liu">LI LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Qing Fang" sort="Qing Fang" uniqKey="Qing Fang" last="Qing Fang">QING FANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Fei Deng" sort="Fei Deng" uniqKey="Fei Deng" last="Fei Deng">FEI DENG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Yi, Christopher E" sort="Yi, Christopher E" uniqKey="Yi C" first="Christopher E." last="Yi">Christopher E. Yi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Lei Ba" sort="Lei Ba" uniqKey="Lei Ba" last="Lei Ba">LEI BA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Wenjie Yu" sort="Wenjie Yu" uniqKey="Wenjie Yu" last="Wenjie Yu">WENJIE YU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Richard D" sort="Lin, Richard D" uniqKey="Lin R" first="Richard D." last="Lin">Richard D. Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Taisheng Li" sort="Taisheng Li" uniqKey="Taisheng Li" last="Taisheng Li">TAISHENG LI</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s2>Beijing 100730</s2><s3>CHN</s3><sZ>9 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Ho, David D" sort="Ho, David D" uniqKey="Ho D" first="David D." last="Ho">David D. Ho</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s2>Beijing 100730</s2><s3>CHN</s3><sZ>9 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhiwei Chen" sort="Zhiwei Chen" uniqKey="Zhiwei Chen" last="Zhiwei Chen">ZHIWEI CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0245100</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0245100 INIST</idno><idno type="RBID">Pascal:07-0245100</idno><idno type="wicri:Area/PascalFrancis/Corpus">000371</idno><idno type="wicri:Area/PascalFrancis/Curation">000618</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000361</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000361</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus</title><author><name sortKey="Li Liu" sort="Li Liu" uniqKey="Li Liu" last="Li Liu">LI LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Qing Fang" sort="Qing Fang" uniqKey="Qing Fang" last="Qing Fang">QING FANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Fei Deng" sort="Fei Deng" uniqKey="Fei Deng" last="Fei Deng">FEI DENG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Yi, Christopher E" sort="Yi, Christopher E" uniqKey="Yi C" first="Christopher E." last="Yi">Christopher E. Yi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Lei Ba" sort="Lei Ba" uniqKey="Lei Ba" last="Lei Ba">LEI BA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Wenjie Yu" sort="Wenjie Yu" uniqKey="Wenjie Yu" last="Wenjie Yu">WENJIE YU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Richard D" sort="Lin, Richard D" uniqKey="Lin R" first="Richard D." last="Lin">Richard D. Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Taisheng Li" sort="Taisheng Li" uniqKey="Taisheng Li" last="Taisheng Li">TAISHENG LI</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s2>Beijing 100730</s2><s3>CHN</s3><sZ>9 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Hubei 430071</wicri:noRegion></affiliation></author><author><name sortKey="Ho, David D" sort="Ho, David D" uniqKey="Ho D" first="David D." last="Ho">David D. Ho</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author><author><name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s2>Beijing 100730</s2><s3>CHN</s3><sZ>9 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhiwei Chen" sort="Zhiwei Chen" uniqKey="Zhiwei Chen" last="Zhiwei Chen">ZHIWEI CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New York, New York 100161</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Cross reaction</term><term>Glycoprotein</term><term>Mutation</term><term>Neutralization</term><term>Severe acute respiratory syndrome</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Mutation</term><term>Glycoprotéine</term><term>Réaction croisée</term><term>Neutralisation</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome (SARS) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. Coronavirus (CoV) found in naturally infected palm civet (civet-CoV) represents the closest genetic relative to SARS-CoV, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. Studies indicate that the receptor binding domain (RBD) of the SARS-CoV spike (S) glycoprotein contains major determinants for viral entry and neutralization. We aim to characterize the impact of natural mutations within the RBDs of civet-CoVs on viral entry and cross-neutralization. In this study, the S glycoprotein genes were recovered from naturally infected civets in central China (Hubei province), extending the geographic distribution of civet-CoV beyond the southeastern province of Guangdong. Moreover, pseudoviruses generated in our laboratory with four civet S genes, each with a distinct RBD, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of SARS-CoV. These four civet S genes were also constructed as DNA vaccines to immunize mice. Immunized sera elicited against most civet S glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing SARS-CoV and vice versa. Convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. Our findings suggest that the design of SARS vaccine should consider not only preventing the reemergence of SARS-CoV but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet CoVs of broad geographic origin.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0></fA01><fA03 i2="1"><s0>J. virol.</s0></fA03><fA05><s2>81</s2></fA05><fA06><s2>9</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus</s1></fA08><fA11 i1="01" i2="1"><s1>LI LIU</s1></fA11><fA11 i1="02" i2="1"><s1>QING FANG</s1></fA11><fA11 i1="03" i2="1"><s1>FEI DENG</s1></fA11><fA11 i1="04" i2="1"><s1>HANZHONG WANG</s1></fA11><fA11 i1="05" i2="1"><s1>YI (Christopher E.)</s1></fA11><fA11 i1="06" i2="1"><s1>LEI BA</s1></fA11><fA11 i1="07" i2="1"><s1>WENJIE YU</s1></fA11><fA11 i1="08" i2="1"><s1>LIN (Richard D.)</s1></fA11><fA11 i1="09" i2="1"><s1>TAISHENG LI</s1></fA11><fA11 i1="10" i2="1"><s1>ZHIHONG HU</s1></fA11><fA11 i1="11" i2="1"><s1>HO (David D.)</s1></fA11><fA11 i1="12" i2="1"><s1>LINQI ZHANG</s1></fA11><fA11 i1="13" i2="1"><s1>ZHIWEI CHEN</s1></fA11><fA14 i1="01"><s1>Aaron Diamond AIDS Research Center, The Rockefeller University</s1><s2>New York, New York 100161</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ></fA14><fA14 i1="02"><s1>State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences</s1><s2>Hubei 430071</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>10 aut.</sZ></fA14><fA14 i1="03"><s1>AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s2>Beijing 100730</s2><s3>CHN</s3><sZ>9 aut.</sZ><sZ>12 aut.</sZ></fA14><fA20><s1>4694-4700</s1></fA20><fA21><s1>2007</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>13592</s2><s5>354000149543340320</s5></fA43><fA44><s0>0000</s0><s1>© 2007 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>31 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>07-0245100</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virology</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>The severe acute respiratory syndrome (SARS) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. Coronavirus (CoV) found in naturally infected palm civet (civet-CoV) represents the closest genetic relative to SARS-CoV, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. Studies indicate that the receptor binding domain (RBD) of the SARS-CoV spike (S) glycoprotein contains major determinants for viral entry and neutralization. We aim to characterize the impact of natural mutations within the RBDs of civet-CoVs on viral entry and cross-neutralization. In this study, the S glycoprotein genes were recovered from naturally infected civets in central China (Hubei province), extending the geographic distribution of civet-CoV beyond the southeastern province of Guangdong. Moreover, pseudoviruses generated in our laboratory with four civet S genes, each with a distinct RBD, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of SARS-CoV. These four civet S genes were also constructed as DNA vaccines to immunize mice. Immunized sera elicited against most civet S glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing SARS-CoV and vice versa. Convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. Our findings suggest that the design of SARS vaccine should consider not only preventing the reemergence of SARS-CoV but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet CoVs of broad geographic origin.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C10</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Mutation</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Mutation</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Mutación</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Glycoprotéine</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Glycoprotein</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Glicoproteína</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Réaction croisée</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Cross reaction</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Reacción cruzada</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Neutralisation</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Neutralization</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Neutralización</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Virologie</s0><s5>09</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Virology</s0><s5>09</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Virología</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>14</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>14</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>14</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>13</s5></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>13</s5></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>13</s5></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>16</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0><s5>16</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>16</s5></fC07><fN21><s1>162</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Li Liu" sort="Li Liu" uniqKey="Li Liu" last="Li Liu">LI LIU</name></noRegion><name sortKey="Ho, David D" sort="Ho, David D" uniqKey="Ho D" first="David D." last="Ho">David D. Ho</name><name sortKey="Lei Ba" sort="Lei Ba" uniqKey="Lei Ba" last="Lei Ba">LEI BA</name><name sortKey="Lin, Richard D" sort="Lin, Richard D" uniqKey="Lin R" first="Richard D." last="Lin">Richard D. Lin</name><name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name><name sortKey="Qing Fang" sort="Qing Fang" uniqKey="Qing Fang" last="Qing Fang">QING FANG</name><name sortKey="Wenjie Yu" sort="Wenjie Yu" uniqKey="Wenjie Yu" last="Wenjie Yu">WENJIE YU</name><name sortKey="Yi, Christopher E" sort="Yi, Christopher E" uniqKey="Yi C" first="Christopher E." last="Yi">Christopher E. Yi</name><name sortKey="Zhiwei Chen" sort="Zhiwei Chen" uniqKey="Zhiwei Chen" last="Zhiwei Chen">ZHIWEI CHEN</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Fei Deng" sort="Fei Deng" uniqKey="Fei Deng" last="Fei Deng">FEI DENG</name></noRegion><name sortKey="Hanzhong Wang" sort="Hanzhong Wang" uniqKey="Hanzhong Wang" last="Hanzhong Wang">HANZHONG WANG</name><name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name><name sortKey="Taisheng Li" sort="Taisheng Li" uniqKey="Taisheng Li" last="Taisheng Li">TAISHENG LI</name><name sortKey="Zhihong Hu" sort="Zhihong Hu" uniqKey="Zhihong Hu" last="Zhihong Hu">ZHIHONG HU</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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