Insight into the evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine Deltacoronavirus functions as a dimer
Identifieur interne : 002F41 ( Ncbi/Merge ); précédent : 002F40; suivant : 002F42Insight into the evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine Deltacoronavirus functions as a dimer
Auteurs : Anjun Zheng ; Yuejun Shi [République populaire de Chine] ; Zhou Shen ; Gang Wang ; Jiale Shi ; Qiqi Xiong [République populaire de Chine] ; Liurong Fang ; Shaobo Xiao ; Zhen F. Fu ; Guiqing PengSource :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2018.
Descripteurs français
- KwdFr :
- Alignement de séquences, Arterivirus (), Arterivirus (génétique), Arterivirus (métabolisme), Clonage moléculaire, Coronavirus (), Coronavirus (génétique), Coronavirus (métabolisme), Cristallographie aux rayons X, Endoribonucleases (), Endoribonucleases (génétique), Endoribonucleases (métabolisme), Escherichia coli (génétique), Escherichia coli (métabolisme), Expression des gènes, Liaison aux protéines, Modèles moléculaires, Motifs et domaines d'intéraction protéique, Multimérisation de protéines, Nidovirales (), Nidovirales (génétique), Nidovirales (métabolisme), Protéines recombinantes (), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Réplication virale (génétique), Similitude de séquences d'acides aminés, Sites de fixation, Sous-unités de protéines (), Sous-unités de protéines (génétique), Sous-unités de protéines (métabolisme), Structure en brin bêta, Structure en hélice alpha, Séquence d'acides aminés, Vecteurs génétiques (), Vecteurs génétiques (métabolisme), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (métabolisme), Évolution moléculaire.
- MESH :
- génétique : Arterivirus, Coronavirus, Endoribonucleases, Escherichia coli, Nidovirales, Protéines recombinantes, Protéines virales non structurales, Réplication virale, Sous-unités de protéines, Virus du SRAS.
- métabolisme : Arterivirus, Coronavirus, Endoribonucleases, Escherichia coli, Nidovirales, Protéines recombinantes, Protéines virales non structurales, Sous-unités de protéines, Vecteurs génétiques, Virus du SRAS.
- Alignement de séquences, Arterivirus, Clonage moléculaire, Coronavirus, Cristallographie aux rayons X, Endoribonucleases, Expression des gènes, Liaison aux protéines, Modèles moléculaires, Motifs et domaines d'intéraction protéique, Multimérisation de protéines, Nidovirales, Protéines recombinantes, Protéines virales non structurales, Similitude de séquences d'acides aminés, Sites de fixation, Sous-unités de protéines, Structure en brin bêta, Structure en hélice alpha, Séquence d'acides aminés, Vecteurs génétiques, Virus du SRAS, Évolution moléculaire.
English descriptors
- KwdEn :
- Amino Acid Sequence, Arterivirus (chemistry), Arterivirus (classification), Arterivirus (genetics), Arterivirus (metabolism), Binding Sites, Cloning, Molecular, Coronavirus (chemistry), Coronavirus (classification), Coronavirus (genetics), Coronavirus (metabolism), Crystallography, X-Ray, Endoribonucleases (chemistry), Endoribonucleases (genetics), Endoribonucleases (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Evolution, Molecular, Gene Expression, Genetic Vectors (chemistry), Genetic Vectors (metabolism), Models, Molecular, Nidovirales (chemistry), Nidovirales (classification), Nidovirales (genetics), Nidovirales (metabolism), Protein Binding, Protein Conformation, alpha-Helical, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Protein Multimerization, Protein Subunits (chemistry), Protein Subunits (genetics), Protein Subunits (metabolism), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (chemistry), SARS Virus (classification), SARS Virus (genetics), SARS Virus (metabolism), Sequence Alignment, Sequence Homology, Amino Acid, Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virus Replication (genetics).
- MESH :
- chemical , chemistry : Endoribonucleases, Protein Subunits, Recombinant Proteins, Viral Nonstructural Proteins.
- chemistry : Arterivirus, Coronavirus, Genetic Vectors, Nidovirales, SARS Virus.
- classification : Arterivirus, Coronavirus, Nidovirales, SARS Virus.
- genetics : Arterivirus, Coronavirus, Endoribonucleases, Escherichia coli, Nidovirales, Protein Subunits, Recombinant Proteins, SARS Virus, Viral Nonstructural Proteins, Virus Replication.
- metabolism : Arterivirus, Coronavirus, Endoribonucleases, Escherichia coli, Genetic Vectors, Nidovirales, Protein Subunits, Recombinant Proteins, SARS Virus, Viral Nonstructural Proteins.
- Amino Acid Sequence, Binding Sites, Cloning, Molecular, Crystallography, X-Ray, Evolution, Molecular, Gene Expression, Models, Molecular, Protein Binding, Protein Conformation, alpha-Helical, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Protein Multimerization, Sequence Alignment, Sequence Homology, Amino Acid.
Abstract
Nidovirus endoribonucleases (NendoUs) include nonstructural protein 15 (nsp15) from coronaviruses and nsp11 from arteriviruses, both of which have been reported to participate in the viral replication process and in the evasion of the host immune system. Results from a previous study of coronaviruses SARS-CoV, HCoV-229E, and MHV nsp15 indicate that it mainly forms a functional hexamer, whereas nsp11 from the arterivirus PRRSV is a dimer. Here, we found that porcine
Url:
DOI: 10.1074/jbc.RA118.003756
PubMed: 29887523
PubMed Central: 6078464
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PMC:6078464Le document en format XML
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evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine <italic>Deltacoronavirus</italic> functions as a dimer</title><author><name sortKey="Zheng, Anjun" sort="Zheng, Anjun" uniqKey="Zheng A" first="Anjun" last="Zheng">Anjun Zheng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Yuejun" sort="Shi, Yuejun" uniqKey="Shi Y" first="Yuejun" last="Shi">Yuejun Shi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="aff3">College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Shen, Zhou" sort="Shen, Zhou" uniqKey="Shen Z" first="Zhou" last="Shen">Zhou Shen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Gang" sort="Wang, Gang" uniqKey="Wang G" first="Gang" last="Wang">Gang Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Jiale" sort="Shi, Jiale" uniqKey="Shi J" first="Jiale" last="Shi">Jiale Shi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Xiong, Qiqi" sort="Xiong, Qiqi" uniqKey="Xiong Q" first="Qiqi" last="Xiong">Qiqi Xiong</name><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="aff3">College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Fang, Liurong" sort="Fang, Liurong" uniqKey="Fang L" first="Liurong" last="Fang">Liurong Fang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Xiao, Shaobo" sort="Xiao, Shaobo" uniqKey="Xiao S" first="Shaobo" last="Xiao">Shaobo Xiao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Fu, Zhen F" sort="Fu, Zhen F" uniqKey="Fu Z" first="Zhen F." last="Fu">Zhen F. Fu</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Peng, Guiqing" sort="Peng, Guiqing" uniqKey="Peng G" first="Guiqing" last="Peng">Guiqing Peng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author></analytic><series><title level="j">The Journal of Biological Chemistry</title><idno type="ISSN">0021-9258</idno><idno type="eISSN">1083-351X</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Arterivirus (chemistry)</term><term>Arterivirus (classification)</term><term>Arterivirus (genetics)</term><term>Arterivirus (metabolism)</term><term>Binding Sites</term><term>Cloning, Molecular</term><term>Coronavirus (chemistry)</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (metabolism)</term><term>Crystallography, X-Ray</term><term>Endoribonucleases (chemistry)</term><term>Endoribonucleases (genetics)</term><term>Endoribonucleases (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Evolution, Molecular</term><term>Gene Expression</term><term>Genetic Vectors (chemistry)</term><term>Genetic Vectors (metabolism)</term><term>Models, Molecular</term><term>Nidovirales (chemistry)</term><term>Nidovirales (classification)</term><term>Nidovirales (genetics)</term><term>Nidovirales (metabolism)</term><term>Protein Binding</term><term>Protein Conformation, alpha-Helical</term><term>Protein Conformation, beta-Strand</term><term>Protein Interaction Domains and Motifs</term><term>Protein Multimerization</term><term>Protein Subunits (chemistry)</term><term>Protein Subunits (genetics)</term><term>Protein Subunits (metabolism)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (classification)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Sequence Alignment</term><term>Sequence Homology, Amino Acid</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Virus Replication (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Arterivirus ()</term><term>Arterivirus (génétique)</term><term>Arterivirus (métabolisme)</term><term>Clonage moléculaire</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (métabolisme)</term><term>Cristallographie aux rayons X</term><term>Endoribonucleases ()</term><term>Endoribonucleases (génétique)</term><term>Endoribonucleases (métabolisme)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Expression des gènes</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Multimérisation de protéines</term><term>Nidovirales ()</term><term>Nidovirales (génétique)</term><term>Nidovirales (métabolisme)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (métabolisme)</term><term>Réplication virale (génétique)</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation</term><term>Sous-unités de protéines ()</term><term>Sous-unités de protéines (génétique)</term><term>Sous-unités de protéines (métabolisme)</term><term>Structure en brin bêta</term><term>Structure en hélice alpha</term><term>Séquence d'acides aminés</term><term>Vecteurs génétiques ()</term><term>Vecteurs génétiques (métabolisme)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Endoribonucleases</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Genetic Vectors</term><term>Nidovirales</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Nidovirales</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Nidovirales</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Viral Nonstructural Proteins</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Nidovirales</term><term>Protéines recombinantes</term><term>Protéines virales non structurales</term><term>Réplication virale</term><term>Sous-unités de protéines</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Genetic Vectors</term><term>Nidovirales</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Nidovirales</term><term>Protéines recombinantes</term><term>Protéines virales non structurales</term><term>Sous-unités de protéines</term><term>Vecteurs génétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Binding Sites</term><term>Cloning, Molecular</term><term>Crystallography, X-Ray</term><term>Evolution, Molecular</term><term>Gene Expression</term><term>Models, Molecular</term><term>Protein Binding</term><term>Protein Conformation, alpha-Helical</term><term>Protein Conformation, beta-Strand</term><term>Protein Interaction Domains and Motifs</term><term>Protein Multimerization</term><term>Sequence Alignment</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Arterivirus</term><term>Clonage moléculaire</term><term>Coronavirus</term><term>Cristallographie aux rayons X</term><term>Endoribonucleases</term><term>Expression des gènes</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Multimérisation de protéines</term><term>Nidovirales</term><term>Protéines recombinantes</term><term>Protéines virales non structurales</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation</term><term>Sous-unités de protéines</term><term>Structure en brin bêta</term><term>Structure en hélice alpha</term><term>Séquence d'acides aminés</term><term>Vecteurs génétiques</term><term>Virus du SRAS</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Nidovirus endoribonucleases (NendoUs) include nonstructural protein 15 (nsp15) from coronaviruses and nsp11 from arteriviruses, both of which have been reported to participate in the viral replication process and in the evasion of the host immune system. Results from a previous study of coronaviruses SARS-CoV, HCoV-229E, and MHV nsp15 indicate that it mainly forms a functional hexamer, whereas nsp11 from the arterivirus PRRSV is a dimer. Here, we found that porcine <italic>Deltacoronavirus</italic> (PDCoV) nsp15 primarily exists as dimers and monomers <italic>in vitro</italic>. Biological experiments reveal that a PDCoV nsp15 mutant lacking the first 27 amino acids of the N-terminal domain (Asn-1–Asn-27) forms more monomers and displays decreased enzymatic activity, indicating that this region is important for its dimerization. Moreover, multiple sequence alignments and three-dimensional structural analysis indicated that the C-terminal region (His-251–Val-261) of PDCoV nsp15 is 10 amino acids shorter and forms a shorter loop than that formed by the equivalent sequence (Gln-259–Phe-279) of SARS-CoV nsp15. This result may explain why PDCoV nsp15 failed to form hexamers. We speculate that NendoUs may have originated from XendoU endoribonucleases (XendoUs) forming monomers in eukaryotic cells, that NendoU from arterivirus gained the ability to form dimers, and that the coronavirus variants then evolved the capacity to assemble into hexamers. We further propose that PDCoV nsp15 may be an intermediate in this evolutionary process. Our findings provide a theoretical basis for improving our understanding of NendoU evolution and offer useful clues for designing drugs and vaccines against nidoviruses.</p></div></front></TEI><double pmid="29887523"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Insight into the evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine <italic>Deltacoronavirus</italic> functions as a dimer</title><author><name sortKey="Zheng, Anjun" sort="Zheng, Anjun" uniqKey="Zheng A" first="Anjun" last="Zheng">Anjun Zheng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Yuejun" sort="Shi, Yuejun" uniqKey="Shi Y" first="Yuejun" last="Shi">Yuejun Shi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="aff3">College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Shen, Zhou" sort="Shen, Zhou" uniqKey="Shen Z" first="Zhou" last="Shen">Zhou Shen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Gang" sort="Wang, Gang" uniqKey="Wang G" first="Gang" last="Wang">Gang Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Jiale" sort="Shi, Jiale" uniqKey="Shi J" first="Jiale" last="Shi">Jiale Shi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Xiong, Qiqi" sort="Xiong, Qiqi" uniqKey="Xiong Q" first="Qiqi" last="Xiong">Qiqi Xiong</name><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="aff3">College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Fang, Liurong" sort="Fang, Liurong" uniqKey="Fang L" first="Liurong" last="Fang">Liurong Fang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Xiao, Shaobo" sort="Xiao, Shaobo" uniqKey="Xiao S" first="Shaobo" last="Xiao">Shaobo Xiao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Fu, Zhen F" sort="Fu, Zhen F" uniqKey="Fu Z" first="Zhen F." last="Fu">Zhen F. Fu</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Peng, Guiqing" sort="Peng, Guiqing" uniqKey="Peng G" first="Guiqing" last="Peng">Guiqing Peng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29887523</idno><idno type="pmc">6078464</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078464</idno><idno type="RBID">PMC:6078464</idno><idno type="doi">10.1074/jbc.RA118.003756</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000C55</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C55</idno><idno type="wicri:Area/Pmc/Curation">000C55</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C55</idno><idno type="wicri:Area/Pmc/Checkpoint">000537</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000537</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Insight into the evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine <italic>Deltacoronavirus</italic> functions as a dimer</title><author><name sortKey="Zheng, Anjun" sort="Zheng, Anjun" uniqKey="Zheng A" first="Anjun" last="Zheng">Anjun Zheng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Yuejun" sort="Shi, Yuejun" uniqKey="Shi Y" first="Yuejun" last="Shi">Yuejun Shi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="aff3">College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Shen, Zhou" sort="Shen, Zhou" uniqKey="Shen Z" first="Zhou" last="Shen">Zhou Shen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Gang" sort="Wang, Gang" uniqKey="Wang G" first="Gang" last="Wang">Gang Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Jiale" sort="Shi, Jiale" uniqKey="Shi J" first="Jiale" last="Shi">Jiale Shi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Xiong, Qiqi" sort="Xiong, Qiqi" uniqKey="Xiong Q" first="Qiqi" last="Xiong">Qiqi Xiong</name><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="aff3">College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Fang, Liurong" sort="Fang, Liurong" uniqKey="Fang L" first="Liurong" last="Fang">Liurong Fang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Xiao, Shaobo" sort="Xiao, Shaobo" uniqKey="Xiao S" first="Shaobo" last="Xiao">Shaobo Xiao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author><author><name sortKey="Fu, Zhen F" sort="Fu, Zhen F" uniqKey="Fu Z" first="Zhen F." last="Fu">Zhen F. Fu</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Peng, Guiqing" sort="Peng, Guiqing" uniqKey="Peng G" first="Guiqing" last="Peng">Guiqing Peng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production</nlm:aff><wicri:noCountry code="subfield">The Cooperative Innovation Center for Sustainable Pig Production</wicri:noCountry></affiliation></author></analytic><series><title level="j">The Journal of Biological Chemistry</title><idno type="ISSN">0021-9258</idno><idno type="eISSN">1083-351X</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Nidovirus endoribonucleases (NendoUs) include nonstructural protein 15 (nsp15) from coronaviruses and nsp11 from arteriviruses, both of which have been reported to participate in the viral replication process and in the evasion of the host immune system. Results from a previous study of coronaviruses SARS-CoV, HCoV-229E, and MHV nsp15 indicate that it mainly forms a functional hexamer, whereas nsp11 from the arterivirus PRRSV is a dimer. Here, we found that porcine <italic>Deltacoronavirus</italic> (PDCoV) nsp15 primarily exists as dimers and monomers <italic>in vitro</italic>. Biological experiments reveal that a PDCoV nsp15 mutant lacking the first 27 amino acids of the N-terminal domain (Asn-1–Asn-27) forms more monomers and displays decreased enzymatic activity, indicating that this region is important for its dimerization. Moreover, multiple sequence alignments and three-dimensional structural analysis indicated that the C-terminal region (His-251–Val-261) of PDCoV nsp15 is 10 amino acids shorter and forms a shorter loop than that formed by the equivalent sequence (Gln-259–Phe-279) of SARS-CoV nsp15. This result may explain why PDCoV nsp15 failed to form hexamers. We speculate that NendoUs may have originated from XendoU endoribonucleases (XendoUs) forming monomers in eukaryotic cells, that NendoU from arterivirus gained the ability to form dimers, and that the coronavirus variants then evolved the capacity to assemble into hexamers. We further propose that PDCoV nsp15 may be an intermediate in this evolutionary process. Our findings provide a theoretical basis for improving our understanding of NendoU evolution and offer useful clues for designing drugs and vaccines against nidoviruses.</p></div></front></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Insight into the evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine <i>Deltacoronavirus</i> functions as a dimer.</title><author><name sortKey="Zheng, Anjun" sort="Zheng, Anjun" uniqKey="Zheng A" first="Anjun" last="Zheng">Anjun Zheng</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Yuejun" sort="Shi, Yuejun" uniqKey="Shi Y" first="Yuejun" last="Shi">Yuejun Shi</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Shen, Zhou" sort="Shen, Zhou" uniqKey="Shen Z" first="Zhou" last="Shen">Zhou Shen</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Gang" sort="Wang, Gang" uniqKey="Wang G" first="Gang" last="Wang">Gang Wang</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Jiale" sort="Shi, Jiale" uniqKey="Shi J" first="Jiale" last="Shi">Jiale Shi</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Xiong, Qiqi" sort="Xiong, Qiqi" uniqKey="Xiong Q" first="Qiqi" last="Xiong">Qiqi Xiong</name><affiliation><nlm:affiliation>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China and.</nlm:affiliation><wicri:noCountry code="subField">China and</wicri:noCountry></affiliation></author><author><name sortKey="Fang, Liurong" sort="Fang, Liurong" uniqKey="Fang L" first="Liurong" last="Fang">Liurong Fang</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Xiao, Shaobo" sort="Xiao, Shaobo" uniqKey="Xiao S" first="Shaobo" last="Xiao">Shaobo Xiao</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Fu, Zhen F" sort="Fu, Zhen F" uniqKey="Fu Z" first="Zhen F" last="Fu">Zhen F. Fu</name><affiliation wicri:level="2"><nlm:affiliation>the Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>the Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Peng, Guiqing" sort="Peng, Guiqing" uniqKey="Peng G" first="Guiqing" last="Peng">Guiqing Peng</name><affiliation wicri:level="1"><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, penggq@mail.hzau.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine</wicri:regionArea><wicri:noRegion>College of Veterinary Medicine</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29887523</idno><idno type="pmid">29887523</idno><idno type="doi">10.1074/jbc.RA118.003756</idno><idno type="wicri:Area/PubMed/Corpus">000997</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000997</idno><idno type="wicri:Area/PubMed/Curation">000997</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000997</idno><idno type="wicri:Area/PubMed/Checkpoint">000996</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000996</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Insight into the evolution of nidovirus endoribonuclease based on the finding that nsp15 from porcine <i>Deltacoronavirus</i> functions as a dimer.</title><author><name sortKey="Zheng, Anjun" sort="Zheng, Anjun" uniqKey="Zheng A" first="Anjun" last="Zheng">Anjun Zheng</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Yuejun" sort="Shi, Yuejun" uniqKey="Shi Y" first="Yuejun" last="Shi">Yuejun Shi</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Shen, Zhou" sort="Shen, Zhou" uniqKey="Shen Z" first="Zhou" last="Shen">Zhou Shen</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Gang" sort="Wang, Gang" uniqKey="Wang G" first="Gang" last="Wang">Gang Wang</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Jiale" sort="Shi, Jiale" uniqKey="Shi J" first="Jiale" last="Shi">Jiale Shi</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Xiong, Qiqi" sort="Xiong, Qiqi" uniqKey="Xiong Q" first="Qiqi" last="Xiong">Qiqi Xiong</name><affiliation><nlm:affiliation>College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China and.</nlm:affiliation><wicri:noCountry code="subField">China and</wicri:noCountry></affiliation></author><author><name sortKey="Fang, Liurong" sort="Fang, Liurong" uniqKey="Fang L" first="Liurong" last="Fang">Liurong Fang</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Xiao, Shaobo" sort="Xiao, Shaobo" uniqKey="Xiao S" first="Shaobo" last="Xiao">Shaobo Xiao</name><affiliation><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine.</nlm:affiliation><wicri:noCountry code="subField">College of Veterinary Medicine</wicri:noCountry></affiliation></author><author><name sortKey="Fu, Zhen F" sort="Fu, Zhen F" uniqKey="Fu Z" first="Zhen F" last="Fu">Zhen F. Fu</name><affiliation wicri:level="2"><nlm:affiliation>the Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>the Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Peng, Guiqing" sort="Peng, Guiqing" uniqKey="Peng G" first="Guiqing" last="Peng">Guiqing Peng</name><affiliation wicri:level="1"><nlm:affiliation>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, penggq@mail.hzau.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>From the State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine</wicri:regionArea><wicri:noRegion>College of Veterinary Medicine</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="eISSN">1083-351X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Arterivirus (chemistry)</term><term>Arterivirus (classification)</term><term>Arterivirus (genetics)</term><term>Arterivirus (metabolism)</term><term>Binding Sites</term><term>Cloning, Molecular</term><term>Coronavirus (chemistry)</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (metabolism)</term><term>Crystallography, X-Ray</term><term>Endoribonucleases (chemistry)</term><term>Endoribonucleases (genetics)</term><term>Endoribonucleases (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Evolution, Molecular</term><term>Gene Expression</term><term>Genetic Vectors (chemistry)</term><term>Genetic Vectors (metabolism)</term><term>Models, Molecular</term><term>Nidovirales (chemistry)</term><term>Nidovirales (classification)</term><term>Nidovirales (genetics)</term><term>Nidovirales (metabolism)</term><term>Protein Binding</term><term>Protein Conformation, alpha-Helical</term><term>Protein Conformation, beta-Strand</term><term>Protein Interaction Domains and Motifs</term><term>Protein Multimerization</term><term>Protein Subunits (chemistry)</term><term>Protein Subunits (genetics)</term><term>Protein Subunits (metabolism)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (classification)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Sequence Alignment</term><term>Sequence Homology, Amino Acid</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Virus Replication (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Arterivirus ()</term><term>Arterivirus (génétique)</term><term>Arterivirus (métabolisme)</term><term>Clonage moléculaire</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (métabolisme)</term><term>Cristallographie aux rayons X</term><term>Endoribonucleases ()</term><term>Endoribonucleases (génétique)</term><term>Endoribonucleases (métabolisme)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Expression des gènes</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Multimérisation de protéines</term><term>Nidovirales ()</term><term>Nidovirales (génétique)</term><term>Nidovirales (métabolisme)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (métabolisme)</term><term>Réplication virale (génétique)</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation</term><term>Sous-unités de protéines ()</term><term>Sous-unités de protéines (génétique)</term><term>Sous-unités de protéines (métabolisme)</term><term>Structure en brin bêta</term><term>Structure en hélice alpha</term><term>Séquence d'acides aminés</term><term>Vecteurs génétiques ()</term><term>Vecteurs génétiques (métabolisme)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Endoribonucleases</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Genetic Vectors</term><term>Nidovirales</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Nidovirales</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Nidovirales</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Viral Nonstructural Proteins</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Nidovirales</term><term>Protéines recombinantes</term><term>Protéines virales non structurales</term><term>Réplication virale</term><term>Sous-unités de protéines</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Genetic Vectors</term><term>Nidovirales</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arterivirus</term><term>Coronavirus</term><term>Endoribonucleases</term><term>Escherichia coli</term><term>Nidovirales</term><term>Protéines recombinantes</term><term>Protéines virales non structurales</term><term>Sous-unités de protéines</term><term>Vecteurs génétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Binding Sites</term><term>Cloning, Molecular</term><term>Crystallography, X-Ray</term><term>Evolution, Molecular</term><term>Gene Expression</term><term>Models, Molecular</term><term>Protein Binding</term><term>Protein Conformation, alpha-Helical</term><term>Protein Conformation, beta-Strand</term><term>Protein Interaction Domains and Motifs</term><term>Protein Multimerization</term><term>Sequence Alignment</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Arterivirus</term><term>Clonage moléculaire</term><term>Coronavirus</term><term>Cristallographie aux rayons X</term><term>Endoribonucleases</term><term>Expression des gènes</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Multimérisation de protéines</term><term>Nidovirales</term><term>Protéines recombinantes</term><term>Protéines virales non structurales</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation</term><term>Sous-unités de protéines</term><term>Structure en brin bêta</term><term>Structure en hélice alpha</term><term>Séquence d'acides aminés</term><term>Vecteurs génétiques</term><term>Virus du SRAS</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nidovirus endoribonucleases (NendoUs) include nonstructural protein 15 (nsp15) from coronaviruses and nsp11 from arteriviruses, both of which have been reported to participate in the viral replication process and in the evasion of the host immune system. Results from a previous study of coronaviruses SARS-CoV, HCoV-229E, and MHV nsp15 indicate that it mainly forms a functional hexamer, whereas nsp11 from the arterivirus PRRSV is a dimer. Here, we found that porcine <i>Deltacoronavirus</i> (PDCoV) nsp15 primarily exists as dimers and monomers <i>in vitro</i> Biological experiments reveal that a PDCoV nsp15 mutant lacking the first 27 amino acids of the N-terminal domain (Asn-1-Asn-27) forms more monomers and displays decreased enzymatic activity, indicating that this region is important for its dimerization. Moreover, multiple sequence alignments and three-dimensional structural analysis indicated that the C-terminal region (His-251-Val-261) of PDCoV nsp15 is 10 amino acids shorter and forms a shorter loop than that formed by the equivalent sequence (Gln-259-Phe-279) of SARS-CoV nsp15. This result may explain why PDCoV nsp15 failed to form hexamers. We speculate that NendoUs may have originated from XendoU endoribonucleases (XendoUs) forming monomers in eukaryotic cells, that NendoU from arterivirus gained the ability to form dimers, and that the coronavirus variants then evolved the capacity to assemble into hexamers. We further propose that PDCoV nsp15 may be an intermediate in this evolutionary process. Our findings provide a theoretical basis for improving our understanding of NendoU evolution and offer useful clues for designing drugs and vaccines against nidoviruses.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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