SARS-like WIV1-CoV poised for human emergence
Identifieur interne : 002C24 ( Ncbi/Merge ); précédent : 002C23; suivant : 002C25SARS-like WIV1-CoV poised for human emergence
Auteurs : Vineet D. Menachery ; Boyd L. Yount ; Amy C. Sims ; Kari Debbink ; Sudhakar S. Agnihothram ; Lisa E. Gralinski ; Rachel L. Graham ; Trevor Scobey ; Jessica A. Plante ; Scott R. Royal ; Jesica Swanstrom ; Timothy P. Sheahan ; Raymond J. Pickles ; Davide Corti [Suisse] ; Scott H. Randell ; Antonio Lanzavecchia [Suisse] ; Wayne A. Marasco ; Ralph S. BaricSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Anticorps neutralisants (immunologie), Cellules Vero, Cellules cultivées, Cellules épithéliales (virologie), Chiroptera (virologie), Conformation des protéines, Coronaviridae (génétique), Coronaviridae (immunologie), Coronaviridae (isolement et purification), Coronaviridae (pathogénicité), Coronaviridae (physiologie), Encéphalite virale (virologie), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (physiologie), Humains, Infections à Coronaviridae (), Infections à Coronaviridae (médecine vétérinaire), Infections à Coronaviridae (transmission), Infections à Coronaviridae (virologie), Maladies transmissibles émergentes (virologie), Modèles moléculaires, Mutation ponctuelle, Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (physiologie), Poumon (cytologie), Protéines de fusion recombinantes (métabolisme), Réactions croisées, Récepteurs viraux (génétique), Récepteurs viraux (physiologie), Réplication virale, Souris, Souris de lignée BALB C, Souris transgéniques, Spécificité d'espèce, Spécificité d'hôte, Virus du SRAS (immunologie), Zoonoses.
- MESH :
- cytologie : Poumon.
- génétique : Coronaviridae, Glycoprotéine de spicule des coronavirus, Peptidyl-Dipeptidase A, Récepteurs viraux.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Anticorps neutralisants, Coronaviridae, Virus du SRAS.
- isolement et purification : Coronaviridae.
- médecine vétérinaire : Infections à Coronaviridae.
- métabolisme : Protéines de fusion recombinantes.
- pathogénicité : Coronaviridae.
- physiologie : Coronaviridae, Glycoprotéine de spicule des coronavirus, Peptidyl-Dipeptidase A, Récepteurs viraux.
- virologie : Cellules épithéliales, Chiroptera, Encéphalite virale, Infections à Coronaviridae, Maladies transmissibles émergentes.
- Animaux, Cellules Vero, Cellules cultivées, Conformation des protéines, Humains, Infections à Coronaviridae, Modèles moléculaires, Mutation ponctuelle, Réactions croisées, Réplication virale, Souris, Souris de lignée BALB C, Souris transgéniques, Spécificité d'espèce, Spécificité d'hôte, Zoonoses.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Cells, Cultured, Chiroptera (virology), Chlorocebus aethiops, Communicable Diseases, Emerging (virology), Coronaviridae (genetics), Coronaviridae (immunology), Coronaviridae (isolation & purification), Coronaviridae (pathogenicity), Coronaviridae (physiology), Coronaviridae Infections (prevention & control), Coronaviridae Infections (transmission), Coronaviridae Infections (veterinary), Coronaviridae Infections (virology), Cross Reactions, Encephalitis, Viral (virology), Epithelial Cells (virology), Host Specificity, Humans, Lung (cytology), Mice, Mice, Inbred BALB C, Mice, Transgenic, Models, Molecular, Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (physiology), Point Mutation, Protein Conformation, Receptors, Virus (genetics), Receptors, Virus (physiology), Recombinant Fusion Proteins (metabolism), SARS Virus (immunology), Species Specificity, Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (physiology), Vero Cells, Virus Replication, Zoonoses.
- MESH :
- chemical , genetics : Peptidyl-Dipeptidase A, Receptors, Virus, Spike Glycoprotein, Coronavirus.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral.
- cytology : Lung.
- genetics : Coronaviridae.
- immunology : Coronaviridae, SARS Virus.
- isolation & purification : Coronaviridae.
- chemical , metabolism : Recombinant Fusion Proteins.
- pathogenicity : Coronaviridae.
- physiology : Coronaviridae, Peptidyl-Dipeptidase A, Receptors, Virus, Spike Glycoprotein, Coronavirus.
- prevention & control : Coronaviridae Infections.
- transmission : Coronaviridae Infections.
- veterinary : Coronaviridae Infections.
- virology : Chiroptera, Communicable Diseases, Emerging, Coronaviridae Infections, Encephalitis, Viral, Epithelial Cells.
- Animals, Cells, Cultured, Chlorocebus aethiops, Cross Reactions, Host Specificity, Humans, Mice, Mice, Inbred BALB C, Mice, Transgenic, Models, Molecular, Point Mutation, Protein Conformation, Species Specificity, Vero Cells, Virus Replication, Zoonoses.
Abstract
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV highlights the continued risk of cross-species transmission leading to epidemic disease. This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. In addition, while monoclonal antibody treatments prove effective, the SARS-based vaccine approach failed to confer protection. Together, the study indicates an ongoing threat posed by WIV1-related viruses and the need for continued study and surveillance.
Url:
DOI: 10.1073/pnas.1517719113
PubMed: 26976607
PubMed Central: 4801244
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PMC:4801244Le document en format XML
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Marasco</name><affiliation><nlm:aff id="aff8">Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26976607</idno><idno type="pmc">4801244</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801244</idno><idno type="RBID">PMC:4801244</idno><idno type="doi">10.1073/pnas.1517719113</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000021</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000021</idno><idno type="wicri:Area/Pmc/Curation">000021</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000021</idno><idno type="wicri:Area/Pmc/Checkpoint">000675</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000675</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:26976607</idno><idno type="wicri:Area/PubMed/Corpus">000C82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000C82</idno><idno type="wicri:Area/PubMed/Curation">000C82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000C82</idno><idno type="wicri:Area/PubMed/Checkpoint">000B93</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000B93</idno><idno type="wicri:Area/Ncbi/Merge">002C24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS-like WIV1-CoV poised for human emergence</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D." last="Menachery">Vineet D. Menachery</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L." last="Yount">Boyd L. Yount</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C." last="Sims">Amy C. Sims</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S." last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E." last="Gralinski">Lisa E. Gralinski</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L." last="Graham">Rachel L. Graham</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A." last="Plante">Jessica A. Plante</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R." last="Royal">Scott R. Royal</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P." last="Sheahan">Timothy P. Sheahan</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J." last="Pickles">Raymond J. Pickles</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Humabs BioMed SA</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H." last="Randell">Scott H. Randell</name><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A." last="Marasco">Wayne A. Marasco</name><affiliation><nlm:aff id="aff8">Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Cells, Cultured</term><term>Chiroptera (virology)</term><term>Chlorocebus aethiops</term><term>Communicable Diseases, Emerging (virology)</term><term>Coronaviridae (genetics)</term><term>Coronaviridae (immunology)</term><term>Coronaviridae (isolation & purification)</term><term>Coronaviridae (pathogenicity)</term><term>Coronaviridae (physiology)</term><term>Coronaviridae Infections (prevention & control)</term><term>Coronaviridae Infections (transmission)</term><term>Coronaviridae Infections (veterinary)</term><term>Coronaviridae Infections (virology)</term><term>Cross Reactions</term><term>Encephalitis, Viral (virology)</term><term>Epithelial Cells (virology)</term><term>Host Specificity</term><term>Humans</term><term>Lung (cytology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Transgenic</term><term>Models, Molecular</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (physiology)</term><term>Point Mutation</term><term>Protein Conformation</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (physiology)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>SARS Virus (immunology)</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (physiology)</term><term>Vero Cells</term><term>Virus Replication</term><term>Zoonoses</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Cellules épithéliales (virologie)</term><term>Chiroptera (virologie)</term><term>Conformation des protéines</term><term>Coronaviridae (génétique)</term><term>Coronaviridae (immunologie)</term><term>Coronaviridae (isolement et purification)</term><term>Coronaviridae (pathogénicité)</term><term>Coronaviridae (physiologie)</term><term>Encéphalite virale (virologie)</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus (physiologie)</term><term>Humains</term><term>Infections à Coronaviridae ()</term><term>Infections à Coronaviridae (médecine vétérinaire)</term><term>Infections à Coronaviridae (transmission)</term><term>Infections à Coronaviridae (virologie)</term><term>Maladies transmissibles émergentes (virologie)</term><term>Modèles moléculaires</term><term>Mutation ponctuelle</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (physiologie)</term><term>Poumon (cytologie)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Réactions croisées</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (physiologie)</term><term>Réplication virale</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris transgéniques</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Virus du SRAS (immunologie)</term><term>Zoonoses</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronaviridae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Anticorps neutralisants</term><term>Coronaviridae</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronaviridae</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronaviridae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronaviridae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronaviridae</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Chiroptera</term><term>Encéphalite virale</term><term>Infections à Coronaviridae</term><term>Maladies transmissibles émergentes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Communicable Diseases, Emerging</term><term>Coronaviridae Infections</term><term>Encephalitis, Viral</term><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chlorocebus aethiops</term><term>Cross Reactions</term><term>Host Specificity</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Transgenic</term><term>Models, Molecular</term><term>Point Mutation</term><term>Protein Conformation</term><term>Species Specificity</term><term>Vero Cells</term><term>Virus Replication</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Conformation des protéines</term><term>Humains</term><term>Infections à Coronaviridae</term><term>Modèles moléculaires</term><term>Mutation ponctuelle</term><term>Réactions croisées</term><term>Réplication virale</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris transgéniques</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Zoonoses</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV highlights the continued risk of cross-species transmission leading to epidemic disease. This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. In addition, while monoclonal antibody treatments prove effective, the SARS-based vaccine approach failed to confer protection. Together, the study indicates an ongoing threat posed by WIV1-related viruses and the need for continued study and surveillance.</p></div></front></TEI><double pmid="26976607"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-like WIV1-CoV poised for human emergence</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D." last="Menachery">Vineet D. Menachery</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L." last="Yount">Boyd L. Yount</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C." last="Sims">Amy C. Sims</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S." last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E." last="Gralinski">Lisa E. Gralinski</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L." last="Graham">Rachel L. Graham</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A." last="Plante">Jessica A. Plante</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R." last="Royal">Scott R. Royal</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P." last="Sheahan">Timothy P. Sheahan</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J." last="Pickles">Raymond J. Pickles</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Humabs BioMed SA</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H." last="Randell">Scott H. Randell</name><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A." last="Marasco">Wayne A. Marasco</name><affiliation><nlm:aff id="aff8">Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26976607</idno><idno type="pmc">4801244</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801244</idno><idno type="RBID">PMC:4801244</idno><idno type="doi">10.1073/pnas.1517719113</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000021</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000021</idno><idno type="wicri:Area/Pmc/Curation">000021</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000021</idno><idno type="wicri:Area/Pmc/Checkpoint">000675</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000675</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS-like WIV1-CoV poised for human emergence</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D." last="Menachery">Vineet D. Menachery</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L." last="Yount">Boyd L. Yount</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C." last="Sims">Amy C. Sims</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S." last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E." last="Gralinski">Lisa E. Gralinski</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L." last="Graham">Rachel L. Graham</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A." last="Plante">Jessica A. Plante</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R." last="Royal">Scott R. Royal</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P." last="Sheahan">Timothy P. Sheahan</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J." last="Pickles">Raymond J. Pickles</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Humabs BioMed SA</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H." last="Randell">Scott H. Randell</name><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A." last="Marasco">Wayne A. Marasco</name><affiliation><nlm:aff id="aff8">Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV highlights the continued risk of cross-species transmission leading to epidemic disease. This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. In addition, while monoclonal antibody treatments prove effective, the SARS-based vaccine approach failed to confer protection. Together, the study indicates an ongoing threat posed by WIV1-related viruses and the need for continued study and surveillance.</p></div></front></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-like WIV1-CoV poised for human emergence.</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S" last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation wicri:level="2"><nlm:affiliation>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arkansas</region></placeName><wicri:cityArea>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson</wicri:cityArea></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L" last="Graham">Rachel L. Graham</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R" last="Royal">Scott R. Royal</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P" last="Sheahan">Timothy P. Sheahan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J" last="Pickles">Raymond J. Pickles</name><affiliation wicri:level="2"><nlm:affiliation>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland; Humabs BioMed SA, Bellinzona, Switzerland;</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland; Humabs BioMed SA, Bellinzona</wicri:regionArea><wicri:noRegion>Bellinzona</wicri:noRegion></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H" last="Randell">Scott H. Randell</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland;</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A" last="Marasco">Wayne A. Marasco</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute-Department of Medicine, Harvard Medical School, Boston MA 02215.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute-Department of Medicine, Harvard Medical School</wicri:cityArea></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name><affiliation wicri:level="4"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; rbaric@email.unc.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson</wicri:regionArea><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName><placeName><settlement type="city">Chapel Hill (Caroline du Nord)</settlement><region type="state">Caroline du Nord</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26976607</idno><idno type="pmid">26976607</idno><idno type="doi">10.1073/pnas.1517719113</idno><idno type="wicri:Area/PubMed/Corpus">000C82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000C82</idno><idno type="wicri:Area/PubMed/Curation">000C82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000C82</idno><idno type="wicri:Area/PubMed/Checkpoint">000B93</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000B93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SARS-like WIV1-CoV poised for human emergence.</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S" last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation wicri:level="2"><nlm:affiliation>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Arkansas</region></placeName><wicri:cityArea>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson</wicri:cityArea></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L" last="Graham">Rachel L. Graham</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R" last="Royal">Scott R. Royal</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P" last="Sheahan">Timothy P. Sheahan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J" last="Pickles">Raymond J. Pickles</name><affiliation wicri:level="2"><nlm:affiliation>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland; Humabs BioMed SA, Bellinzona, Switzerland;</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland; Humabs BioMed SA, Bellinzona</wicri:regionArea><wicri:noRegion>Bellinzona</wicri:noRegion></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H" last="Randell">Scott H. Randell</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:affiliation>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland;</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich</wicri:regionArea><wicri:noRegion>Zurich</wicri:noRegion></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A" last="Marasco">Wayne A. Marasco</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute-Department of Medicine, Harvard Medical School, Boston MA 02215.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute-Department of Medicine, Harvard Medical School</wicri:cityArea></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name><affiliation wicri:level="4"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; rbaric@email.unc.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson</wicri:regionArea><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName><placeName><settlement type="city">Chapel Hill (Caroline du Nord)</settlement><region type="state">Caroline du Nord</region></placeName></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Cells, Cultured</term><term>Chiroptera (virology)</term><term>Chlorocebus aethiops</term><term>Communicable Diseases, Emerging (virology)</term><term>Coronaviridae (genetics)</term><term>Coronaviridae (immunology)</term><term>Coronaviridae (isolation & purification)</term><term>Coronaviridae (pathogenicity)</term><term>Coronaviridae (physiology)</term><term>Coronaviridae Infections (prevention & control)</term><term>Coronaviridae Infections (transmission)</term><term>Coronaviridae Infections (veterinary)</term><term>Coronaviridae Infections (virology)</term><term>Cross Reactions</term><term>Encephalitis, Viral (virology)</term><term>Epithelial Cells (virology)</term><term>Host Specificity</term><term>Humans</term><term>Lung (cytology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Transgenic</term><term>Models, Molecular</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (physiology)</term><term>Point Mutation</term><term>Protein Conformation</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (physiology)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>SARS Virus (immunology)</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (physiology)</term><term>Vero Cells</term><term>Virus Replication</term><term>Zoonoses</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Cellules épithéliales (virologie)</term><term>Chiroptera (virologie)</term><term>Conformation des protéines</term><term>Coronaviridae (génétique)</term><term>Coronaviridae (immunologie)</term><term>Coronaviridae (isolement et purification)</term><term>Coronaviridae (pathogénicité)</term><term>Coronaviridae (physiologie)</term><term>Encéphalite virale (virologie)</term><term>Glycoprotéine de spicule des coronavirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus (physiologie)</term><term>Humains</term><term>Infections à Coronaviridae ()</term><term>Infections à Coronaviridae (médecine vétérinaire)</term><term>Infections à Coronaviridae (transmission)</term><term>Infections à Coronaviridae (virologie)</term><term>Maladies transmissibles émergentes (virologie)</term><term>Modèles moléculaires</term><term>Mutation ponctuelle</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (physiologie)</term><term>Poumon (cytologie)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Réactions croisées</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (physiologie)</term><term>Réplication virale</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris transgéniques</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Virus du SRAS (immunologie)</term><term>Zoonoses</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronaviridae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Anticorps neutralisants</term><term>Coronaviridae</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronaviridae</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronaviridae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronaviridae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronaviridae</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Chiroptera</term><term>Encéphalite virale</term><term>Infections à Coronaviridae</term><term>Maladies transmissibles émergentes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Communicable Diseases, Emerging</term><term>Coronaviridae Infections</term><term>Encephalitis, Viral</term><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Chlorocebus aethiops</term><term>Cross Reactions</term><term>Host Specificity</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Transgenic</term><term>Models, Molecular</term><term>Point Mutation</term><term>Protein Conformation</term><term>Species Specificity</term><term>Vero Cells</term><term>Virus Replication</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Conformation des protéines</term><term>Humains</term><term>Infections à Coronaviridae</term><term>Modèles moléculaires</term><term>Mutation ponctuelle</term><term>Réactions croisées</term><term>Réplication virale</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris transgéniques</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Zoonoses</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Outbreaks from zoonotic sources represent a threat to both human disease as well as the global economy. Despite a wealth of metagenomics studies, methods to leverage these datasets to identify future threats are underdeveloped. In this study, we describe an approach that combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emergence and pathogenic potential of circulating zoonotic viruses. Focusing on the severe acute respiratory syndrome (SARS)-like viruses, the results indicate that the WIV1-coronavirus (CoV) cluster has the ability to directly infect and may undergo limited transmission in human populations. However, in vivo attenuation suggests additional adaptation is required for epidemic disease. Importantly, available SARS monoclonal antibodies offered success in limiting viral infection absent from available vaccine approaches. Together, the data highlight the utility of a platform to identify and prioritize prepandemic strains harbored in animal reservoirs and document the threat posed by WIV1-CoV for emergence in human populations. </div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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