SARS-like WIV1-CoV poised for human emergence
Identifieur interne : 000021 ( Pmc/Curation ); précédent : 000020; suivant : 000022SARS-like WIV1-CoV poised for human emergence
Auteurs : Vineet D. Menachery ; Boyd L. Yount ; Amy C. Sims ; Kari Debbink ; Sudhakar S. Agnihothram ; Lisa E. Gralinski ; Rachel L. Graham ; Trevor Scobey ; Jessica A. Plante ; Scott R. Royal ; Jesica Swanstrom ; Timothy P. Sheahan ; Raymond J. Pickles ; Davide Corti [Suisse] ; Scott H. Randell ; Antonio Lanzavecchia [Suisse] ; Wayne A. Marasco ; Ralph S. BaricSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2016.
Abstract
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV highlights the continued risk of cross-species transmission leading to epidemic disease. This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. In addition, while monoclonal antibody treatments prove effective, the SARS-based vaccine approach failed to confer protection. Together, the study indicates an ongoing threat posed by WIV1-related viruses and the need for continued study and surveillance.
Url:
DOI: 10.1073/pnas.1517719113
PubMed: 26976607
PubMed Central: 4801244
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-like WIV1-CoV poised for human emergence</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D." last="Menachery">Vineet D. Menachery</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L." last="Yount">Boyd L. Yount</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C." last="Sims">Amy C. Sims</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S." last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E." last="Gralinski">Lisa E. Gralinski</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L." last="Graham">Rachel L. Graham</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A." last="Plante">Jessica A. Plante</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R." last="Royal">Scott R. Royal</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P." last="Sheahan">Timothy P. Sheahan</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J." last="Pickles">Raymond J. Pickles</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Humabs BioMed SA</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H." last="Randell">Scott H. Randell</name><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A." last="Marasco">Wayne A. Marasco</name><affiliation><nlm:aff id="aff8">Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26976607</idno><idno type="pmc">4801244</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801244</idno><idno type="RBID">PMC:4801244</idno><idno type="doi">10.1073/pnas.1517719113</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000021</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000021</idno><idno type="wicri:Area/Pmc/Curation">000021</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000021</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS-like WIV1-CoV poised for human emergence</title><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D." last="Menachery">Vineet D. Menachery</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L." last="Yount">Boyd L. Yount</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C." last="Sims">Amy C. Sims</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Debbink, Kari" sort="Debbink, Kari" uniqKey="Debbink K" first="Kari" last="Debbink">Kari Debbink</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Agnihothram, Sudhakar S" sort="Agnihothram, Sudhakar S" uniqKey="Agnihothram S" first="Sudhakar S." last="Agnihothram">Sudhakar S. Agnihothram</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E." last="Gralinski">Lisa E. Gralinski</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L." last="Graham">Rachel L. Graham</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A." last="Plante">Jessica A. Plante</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Royal, Scott R" sort="Royal, Scott R" uniqKey="Royal S" first="Scott R." last="Royal">Scott R. Royal</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Swanstrom, Jesica" sort="Swanstrom, Jesica" uniqKey="Swanstrom J" first="Jesica" last="Swanstrom">Jesica Swanstrom</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Sheahan, Timothy P" sort="Sheahan, Timothy P" uniqKey="Sheahan T" first="Timothy P." last="Sheahan">Timothy P. Sheahan</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Pickles, Raymond J" sort="Pickles, Raymond J" uniqKey="Pickles R" first="Raymond J." last="Pickles">Raymond J. Pickles</name><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>Humabs BioMed SA</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Randell, Scott H" sort="Randell, Scott H" uniqKey="Randell S" first="Scott H." last="Randell">Scott H. Randell</name><affiliation><nlm:aff id="aff4">Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marasco, Wayne A" sort="Marasco, Wayne A" uniqKey="Marasco W" first="Wayne A." last="Marasco">Wayne A. Marasco</name><affiliation><nlm:aff id="aff8">Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation><nlm:aff id="aff1">Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Significance</title><p>The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV highlights the continued risk of cross-species transmission leading to epidemic disease. This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. In addition, while monoclonal antibody treatments prove effective, the SARS-based vaccine approach failed to confer protection. Together, the study indicates an ongoing threat posed by WIV1-related viruses and the need for continued study and surveillance.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="iso-abbrev">Proc. Natl. Acad. Sci. U.S.A</journal-id><journal-id journal-id-type="hwp">pnas</journal-id><journal-id journal-id-type="pmc">pnas</journal-id><journal-id journal-id-type="publisher-id">PNAS</journal-id><journal-title-group><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title></journal-title-group><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26976607</article-id><article-id pub-id-type="pmc">4801244</article-id><article-id pub-id-type="publisher-id">201517719</article-id><article-id pub-id-type="doi">10.1073/pnas.1517719113</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Microbiology</subject></subj-group></subj-group><series-title>From the Cover</series-title></article-categories><title-group><article-title>SARS-like WIV1-CoV poised for human emergence</article-title><alt-title alt-title-type="short">SARS-like WIV1-CoV poised for human emergence</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Menachery</surname><given-names>Vineet D.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yount</surname><given-names>Boyd L.</given-names><suffix>Jr.</suffix></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sims</surname><given-names>Amy C.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Debbink</surname><given-names>Kari</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Agnihothram</surname><given-names>Sudhakar S.</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gralinski</surname><given-names>Lisa E.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Graham</surname><given-names>Rachel L.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Scobey</surname><given-names>Trevor</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Plante</surname><given-names>Jessica A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Royal</surname><given-names>Scott R.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Swanstrom</surname><given-names>Jesica</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sheahan</surname><given-names>Timothy P.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pickles</surname><given-names>Raymond J.</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Corti</surname><given-names>Davide</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref><xref ref-type="aff" rid="aff6"><sup>f</sup></xref><xref ref-type="aff" rid="aff7"><sup>g</sup></xref></contrib><contrib contrib-type="author"><name><surname>Randell</surname><given-names>Scott H.</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lanzavecchia</surname><given-names>Antonio</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Marasco</surname><given-names>Wayne A.</given-names></name><xref ref-type="aff" rid="aff8"><sup>h</sup></xref></contrib><contrib contrib-type="author"><name><surname>Baric</surname><given-names>Ralph S.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1"><sup>a</sup>Department of Epidemiology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</aff><aff id="aff2"><sup>b</sup>Department of Microbiology and Immunology,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</aff><aff id="aff3"><sup>c</sup>Division of Microbiology, National Center for Toxicological Research,<institution>Food and Drug Administration</institution>, Jefferson,<addr-line>AR</addr-line> 72079;</aff><aff id="aff4"><sup>d</sup>Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center,<institution>University of North Carolina at Chapel Hill</institution>, Chapel Hill,<addr-line>NC</addr-line> 27599;</aff><aff id="aff5"><sup>e</sup><institution>Institute for Research in Biomedicine</institution>, Bellinzona,<country>Switzerland</country>;</aff><aff id="aff6"><sup>f</sup>Institute of Microbiology,<institution>Eidgenössische Technische Hochschule Zurich</institution>, Zurich,<country>Switzerland</country>;</aff><aff id="aff7"><sup>g</sup><institution>Humabs BioMed SA</institution>, Bellinzona,<country>Switzerland</country>;</aff><aff id="aff8"><sup>h</sup>Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute–Department of Medicine,<institution>Harvard Medical School</institution>, Boston<addr-line>MA</addr-line> 02215</aff></contrib-group><author-notes><corresp id="cor1"><sup>1</sup>To whom correspondence should be addressed. Email: <email>rbaric@email.unc.edu</email>.</corresp><fn fn-type="edited-by"><p>Edited by Peter Palese, Icahn School of Medicine at Mount Sinai, New York, NY, and approved January 6, 2016 (received for review September 4, 2015)</p></fn><fn fn-type="con"><p>Author contributions: V.D.M., B.L.Y., and R.S.B. designed research; V.D.M., B.L.Y., A.C.S., S.S.A., L.E.G., T.S., J.A.P., S.R.R., J.S., and T.P.S. performed research; V.D.M., B.L.Y., R.J.P., D.C., S.H.R., A.L., and W.A.M. contributed new reagents/analytic tools; V.D.M., A.C.S., K.D., R.L.G., and R.S.B. analyzed data; and V.D.M. and R.S.B. wrote the paper.</p></fn><fn fn-type="conflict"><p>The authors declare no conflict of interest.</p></fn></author-notes><pub-date pub-type="ppub"><day>15</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>14</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>14</day><month>3</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>113</volume><issue>11</issue><fpage>3048</fpage><lpage>3053</lpage><permissions><license license-type="open-access"><license-p>Freely available online through the PNAS open access option.</license-p></license></permissions><self-uri xlink:title="pdf" xlink:href="pnas.201517719.pdf"></self-uri><abstract abstract-type="executive-summary"><title>Significance</title><p>The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV highlights the continued risk of cross-species transmission leading to epidemic disease. This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. In addition, while monoclonal antibody treatments prove effective, the SARS-based vaccine approach failed to confer protection. Together, the study indicates an ongoing threat posed by WIV1-related viruses and the need for continued study and surveillance.</p></abstract><abstract><p>Outbreaks from zoonotic sources represent a threat to both human disease as well as the global economy. Despite a wealth of metagenomics studies, methods to leverage these datasets to identify future threats are underdeveloped. In this study, we describe an approach that combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emergence and pathogenic potential of circulating zoonotic viruses. Focusing on the severe acute respiratory syndrome (SARS)-like viruses, the results indicate that the WIV1-coronavirus (CoV) cluster has the ability to directly infect and may undergo limited transmission in human populations. However, in vivo attenuation suggests additional adaptation is required for epidemic disease. Importantly, available SARS monoclonal antibodies offered success in limiting viral infection absent from available vaccine approaches. Together, the data highlight the utility of a platform to identify and prioritize prepandemic strains harbored in animal reservoirs and document the threat posed by WIV1-CoV for emergence in human populations.</p></abstract><kwd-group><kwd>SARS</kwd><kwd>CoV</kwd><kwd>emergence</kwd><kwd>Spike</kwd><kwd>WIV1</kwd></kwd-group><funding-group><award-group id="gs1"><funding-source id="sp1">HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)<named-content content-type="funder-id">100000060</named-content></funding-source><award-id rid="sp1">U19AI109761</award-id></award-group><award-group id="gs2"><funding-source id="sp2">HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)<named-content content-type="funder-id">100000060</named-content></funding-source><award-id rid="sp2">U19AI107810</award-id></award-group><award-group id="gs3"><funding-source id="sp3">Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID)<named-content content-type="funder-id">100006492</named-content></funding-source><award-id rid="sp3">AI1085524</award-id></award-group><award-group id="gs4"><funding-source id="sp4">HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)<named-content content-type="funder-id">100000060</named-content></funding-source><award-id rid="sp4">F32AI102561</award-id></award-group><award-group id="gs5"><funding-source id="sp5">HHS | NIH | National Institute on Aging (U.S. National Institute on Aging)<named-content content-type="funder-id">100000049</named-content></funding-source><award-id rid="sp5">K99AG049092</award-id></award-group><award-group id="gs6"><funding-source id="sp6">HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)<named-content content-type="funder-id">100000062</named-content></funding-source><award-id rid="sp6">DK065988</award-id></award-group></funding-group><counts><page-count count="6"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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