SrasV1, Ncbi, Merge, bibRecord, 002613

Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.

Identifieur interne : 002613 ( Ncbi/Merge ); précédent : 002612; suivant : 002614

Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.

Auteurs : Ewan P. Plant [États-Unis] ; Amy C. Sims ; Ralph S. Baric ; Jonathan D. Dinman ; Deborah R. Taylor

Source :

Viruses [ 1999-4915 ] ; 2013.

RBID : pubmed:23334702

Descripteurs français

KwdFr :
- ARN viral (génétique), ARN viral (métabolisme), Décalage ribosomique du cadre de lecture, Génome viral, Humains, Mutation avec décalage du cadre de lecture, Protéines virales (génétique), Protéines virales (métabolisme), Réplication virale, Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- génétique : ARN viral, Protéines virales, Virus du SRAS.
- métabolisme : ARN viral, Protéines virales.
- physiologie : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Décalage ribosomique du cadre de lecture, Génome viral, Humains, Mutation avec décalage du cadre de lecture, Réplication virale.

English descriptors

KwdEn :
- Frameshift Mutation, Frameshifting, Ribosomal, Genome, Viral, Humans, RNA, Viral (genetics), RNA, Viral (metabolism), SARS Virus (genetics), SARS Virus (physiology), Severe Acute Respiratory Syndrome (virology), Viral Proteins (genetics), Viral Proteins (metabolism), Virus Replication.
MESH :
- chemical , genetics : RNA, Viral, Viral Proteins.
- chemical , metabolism : RNA, Viral, Viral Proteins.
- genetics : SARS Virus.
- physiology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Frameshift Mutation, Frameshifting, Ribosomal, Genome, Viral, Humans, Virus Replication.

Abstract

In previous studies, differences in the amount of genomic and subgenomic RNA produced by coronaviruses with mutations in the programmed ribosomal frameshift signal of ORF1a/b were observed. It was not clear if these differences were due to changes in genomic sequence, the protein sequence or the frequency of frameshifting. Here, viruses with synonymous codon changes are shown to produce different ratios of genomic and subgenomic RNA. These findings demonstrate that the protein sequence is not the primary cause of altered genomic and subgenomic RNA production. The synonymous codon changes affect both the structure of the frameshift signal and frameshifting efficiency. Small differences in frameshifting efficiency result in dramatic differences in genomic RNA production and TCID50 suggesting that the frameshifting frequency must stay above a certain threshold for optimal virus production. The data suggest that either the RNA sequence or the ratio of viral proteins resulting from different levels of frameshifting affects viral replication.

DOI: 10.3390/v5010279
PubMed: 23334702

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 001258
to stream PubMed, to step Curation: 001258
to stream PubMed, to step Checkpoint: 001233

Links to Exploration step

pubmed:23334702

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.</title>
<author><name sortKey="Plant, Ewan P" sort="Plant, Ewan P" uniqKey="Plant E" first="Ewan P" last="Plant">Ewan P. Plant</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Emerging Pathogens, Division of Transfusion-Transmitted Diseases, Food and Drug Administration, Bethesda, Maryland 20892, USA. Ewan.Plant@fda.hhs.gov</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Emerging Pathogens, Division of Transfusion-Transmitted Diseases, Food and Drug Administration, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name>
</author>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
</author>
<author><name sortKey="Dinman, Jonathan D" sort="Dinman, Jonathan D" uniqKey="Dinman J" first="Jonathan D" last="Dinman">Jonathan D. Dinman</name>
</author>
<author><name sortKey="Taylor, Deborah R" sort="Taylor, Deborah R" uniqKey="Taylor D" first="Deborah R" last="Taylor">Deborah R. Taylor</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23334702</idno>
<idno type="pmid">23334702</idno>
<idno type="doi">10.3390/v5010279</idno>
<idno type="wicri:Area/PubMed/Corpus">001258</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001258</idno>
<idno type="wicri:Area/PubMed/Curation">001258</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001258</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001233</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001233</idno>
<idno type="wicri:Area/Ncbi/Merge">002613</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.</title>
<author><name sortKey="Plant, Ewan P" sort="Plant, Ewan P" uniqKey="Plant E" first="Ewan P" last="Plant">Ewan P. Plant</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Emerging Pathogens, Division of Transfusion-Transmitted Diseases, Food and Drug Administration, Bethesda, Maryland 20892, USA. Ewan.Plant@fda.hhs.gov</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Emerging Pathogens, Division of Transfusion-Transmitted Diseases, Food and Drug Administration, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name>
</author>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
</author>
<author><name sortKey="Dinman, Jonathan D" sort="Dinman, Jonathan D" uniqKey="Dinman J" first="Jonathan D" last="Dinman">Jonathan D. Dinman</name>
</author>
<author><name sortKey="Taylor, Deborah R" sort="Taylor, Deborah R" uniqKey="Taylor D" first="Deborah R" last="Taylor">Deborah R. Taylor</name>
</author>
</analytic>
<series><title level="j">Viruses</title>
<idno type="eISSN">1999-4915</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Frameshift Mutation</term>
<term>Frameshifting, Ribosomal</term>
<term>Genome, Viral</term>
<term>Humans</term>
<term>RNA, Viral (genetics)</term>
<term>RNA, Viral (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Viral Proteins (genetics)</term>
<term>Viral Proteins (metabolism)</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>ARN viral (métabolisme)</term>
<term>Décalage ribosomique du cadre de lecture</term>
<term>Génome viral</term>
<term>Humains</term>
<term>Mutation avec décalage du cadre de lecture</term>
<term>Protéines virales (génétique)</term>
<term>Protéines virales (métabolisme)</term>
<term>Réplication virale</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Protéines virales</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term>
<term>Protéines virales</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Frameshift Mutation</term>
<term>Frameshifting, Ribosomal</term>
<term>Genome, Viral</term>
<term>Humans</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Décalage ribosomique du cadre de lecture</term>
<term>Génome viral</term>
<term>Humains</term>
<term>Mutation avec décalage du cadre de lecture</term>
<term>Réplication virale</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In previous studies, differences in the amount of genomic and subgenomic RNA produced by coronaviruses with mutations in the programmed ribosomal frameshift signal of ORF1a/b were observed. It was not clear if these differences were due to changes in genomic sequence, the protein sequence or the frequency of frameshifting. Here, viruses with synonymous codon changes are shown to produce different ratios of genomic and subgenomic RNA. These findings demonstrate that the protein sequence is not the primary cause of altered genomic and subgenomic RNA production. The synonymous codon changes affect both the structure of the frameshift signal and frameshifting efficiency. Small differences in frameshifting efficiency result in dramatic differences in genomic RNA production and TCID50 suggesting that the frameshifting frequency must stay above a certain threshold for optimal virus production. The data suggest that either the RNA sequence or the ratio of viral proteins resulting from different levels of frameshifting affects viral replication.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23334702</PMID>
<DateCompleted><Year>2013</Year>
<Month>06</Month>
<Day>24</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>5</Volume>
<Issue>1</Issue>
<PubDate><Year>2013</Year>
<Month>Jan</Month>
<Day>18</Day>
</PubDate>
</JournalIssue>
<Title>Viruses</Title>
<ISOAbbreviation>Viruses</ISOAbbreviation>
</Journal>
<ArticleTitle>Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.</ArticleTitle>
<Pagination><MedlinePgn>279-94</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/v5010279</ELocationID>
<Abstract><AbstractText>In previous studies, differences in the amount of genomic and subgenomic RNA produced by coronaviruses with mutations in the programmed ribosomal frameshift signal of ORF1a/b were observed. It was not clear if these differences were due to changes in genomic sequence, the protein sequence or the frequency of frameshifting. Here, viruses with synonymous codon changes are shown to produce different ratios of genomic and subgenomic RNA. These findings demonstrate that the protein sequence is not the primary cause of altered genomic and subgenomic RNA production. The synonymous codon changes affect both the structure of the frameshift signal and frameshifting efficiency. Small differences in frameshifting efficiency result in dramatic differences in genomic RNA production and TCID50 suggesting that the frameshifting frequency must stay above a certain threshold for optimal virus production. The data suggest that either the RNA sequence or the ratio of viral proteins resulting from different levels of frameshifting affects viral replication.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Plant</LastName>
<ForeName>Ewan P</ForeName>
<Initials>EP</Initials>
<AffiliationInfo><Affiliation>Laboratory of Emerging Pathogens, Division of Transfusion-Transmitted Diseases, Food and Drug Administration, Bethesda, Maryland 20892, USA. Ewan.Plant@fda.hhs.gov</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sims</LastName>
<ForeName>Amy C</ForeName>
<Initials>AC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Baric</LastName>
<ForeName>Ralph S</ForeName>
<Initials>RS</Initials>
</Author>
<Author ValidYN="Y"><LastName>Dinman</LastName>
<ForeName>Jonathan D</ForeName>
<Initials>JD</Initials>
</Author>
<Author ValidYN="Y"><LastName>Taylor</LastName>
<ForeName>Deborah R</ForeName>
<Initials>DR</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>R01 AI064307</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>AI064307</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2013</Year>
<Month>01</Month>
<Day>18</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Viruses</MedlineTA>
<NlmUniqueID>101509722</NlmUniqueID>
<ISSNLinking>1999-4915</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016368" MajorTopicYN="N">Frameshift Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018965" MajorTopicYN="Y">Frameshifting, Ribosomal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year>
<Month>12</Month>
<Day>06</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2013</Year>
<Month>01</Month>
<Day>14</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2013</Year>
<Month>01</Month>
<Day>15</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2013</Year>
<Month>1</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2013</Year>
<Month>1</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2013</Year>
<Month>6</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">23334702</ArticleId>
<ArticleId IdType="pii">v5010279</ArticleId>
<ArticleId IdType="doi">10.3390/v5010279</ArticleId>
<ArticleId IdType="pmc">PMC3564121</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11660-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7526401</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA Biol. 2011 Mar-Apr;8(2):237-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21378501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1996 Jul 26;260(4):479-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8759314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Microbiol. 2012 Aug 06;3:279</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22888327</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Feb;78(3):1352-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14722290</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12995-3000</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14569023</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Jan;81(1):20-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16928755</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Apr;86(8):4444-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22318142</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2011 Sep;7(9):e1002215</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21931546</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1989 May 19;57(4):537-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2720781</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Virol. 2011 Nov;1(5):339-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22140418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2005 Jun;3(6):e172</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15884978</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2005 Feb 20;332(2):498-510</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15680415</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2010 May 25;401(1):29-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20202661</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2010 Mar 26;397(2):448-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20114053</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1993 Dec 25;21(25):5838-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8290341</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Feb;40(4):1737-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22039154</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 May;84(9):4330-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20164235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Jun;81(12):6771-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17428856</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004;32(20):e160</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15561995</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2010 Jan;38(1):203-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19875418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2006 Apr;12(4):666-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16497657</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2003 Oct;9(10):1246-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">13130138</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2006 Aug;8(8):1211-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16803585</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2006 Oct 18;25(20):4933-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17024178</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2006;1(3):1610-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17406453</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2005;33(13):4265-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16055920</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1992 Jun;66(6):3669-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1583726</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1991 Aug 20;220(4):889-902</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1880803</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2012 Sep 21;422(3):328-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22705285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2000 Nov;74(22):10359-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11044080</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Virus Res. 2006;66:193-292</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16877062</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2013 Feb 1;41(4):2594-608</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23275571</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Top Microbiol Immunol. 2005;287:57-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15609509</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
<name sortKey="Dinman, Jonathan D" sort="Dinman, Jonathan D" uniqKey="Dinman J" first="Jonathan D" last="Dinman">Jonathan D. Dinman</name>
<name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name>
<name sortKey="Taylor, Deborah R" sort="Taylor, Deborah R" uniqKey="Taylor D" first="Deborah R" last="Taylor">Deborah R. Taylor</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Plant, Ewan P" sort="Plant, Ewan P" uniqKey="Plant E" first="Ewan P" last="Plant">Ewan P. Plant</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002613 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 002613 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     pubmed:23334702
   |texte=   Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:23334702" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.

Altering SARS coronavirus frameshift efficiency affects genomic and subgenomic RNA production.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki