A conserved RNA pseudoknot in a putative molecular switch domain of the 3'-untranslated region of coronaviruses is only marginally stable.
Identifieur interne : 002369 ( Ncbi/Merge ); précédent : 002368; suivant : 002370A conserved RNA pseudoknot in a putative molecular switch domain of the 3'-untranslated region of coronaviruses is only marginally stable.
Auteurs : Suzanne N. Stammler [États-Unis] ; Song Cao ; Shi-Jie Chen ; David P. GiedrocSource :
- RNA (New York, N.Y.) [ 1469-9001 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (génétique), Appariement de bases, Conformation d'acide nucléique, Données de séquences moléculaires, Génome viral, Régions 3' non traduites, Réplication virale (génétique), Séquence nucléotidique, Thermodynamique, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (physiologie), Virus du SRAS (génétique).
- MESH :
- génétique : ARN viral, Réplication virale, Virus de l'hépatite murine, Virus du SRAS.
- physiologie : Virus de l'hépatite murine.
- ARN viral, Appariement de bases, Conformation d'acide nucléique, Données de séquences moléculaires, Génome viral, Régions 3' non traduites, Séquence nucléotidique, Thermodynamique.
English descriptors
- KwdEn :
- 3' Untranslated Regions, Base Pairing, Base Sequence, Genome, Viral, Molecular Sequence Data, Murine hepatitis virus (genetics), Murine hepatitis virus (physiology), Nucleic Acid Conformation, RNA, Viral (chemistry), RNA, Viral (genetics), SARS Virus (genetics), Thermodynamics, Virus Replication (genetics).
- MESH :
- chemical , chemistry : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical : 3' Untranslated Regions.
- genetics : Murine hepatitis virus, SARS Virus, Virus Replication.
- physiology : Murine hepatitis virus.
- Base Pairing, Base Sequence, Genome, Viral, Molecular Sequence Data, Nucleic Acid Conformation, Thermodynamics.
Abstract
The 3'-untranslated region (UTR) of the group 2 coronavirus mouse hepatitis virus (MHV) genome contains a predicted bulged stem-loop (designated P0ab), a conserved cis-acting pseudoknot (PK), and a more distal stem-loop (designated P2). Base-pairing to create the pseudoknot-forming stem (P1(pk)) is mutually exclusive with formation of stem P0a at the base of the bulged stem-loop; as a result, the two structures cannot be present simultaneously. Herein, we use thermodynamic methods to evaluate the ability of individual subdomains of the 3' UTR to adopt a pseudoknotted conformation. We find that an RNA capable of forming only the predicted PK (58 nt; 3' nucleotides 241-185) adopts the P2 stem-loop with little evidence for P1(pk) pairing in 0.1 M KCl and the absence of Mg(2+); as Mg(2+) or 1 M KCl is added, a new thermal unfolding transition is induced and assignable to P1(pk) pairing. The P1(pk) helix is only marginally stable, ΔG(25) ≈ 1.2 ± 0.3 kcal/mol (5.0 mM Mg(2+), 100 mM K(+)), and unfolded at 37°C. Similar findings characterize an RNA 5' extended through the P0b helix only (89 nt; 294-185). In contrast, an RNA capable of forming either the P0a helix or the pseudoknot (97 nt; 301-185) forms no P1(pk) helix. Thermal unfolding simulations are fully consistent with these experimental findings. These data reveal that the PK forms weakly and only when the competing double-hairpin structure cannot form; in the UTR RNA, the double hairpin is the predominant conformer under all solution conditions.
DOI: 10.1261/rna.2816711
PubMed: 21799029
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001486
- to stream PubMed, to step Curation: 001486
- to stream PubMed, to step Checkpoint: 001540
Links to Exploration step
pubmed:21799029Le document en format XML
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<front><div type="abstract" xml:lang="en">The 3'-untranslated region (UTR) of the group 2 coronavirus mouse hepatitis virus (MHV) genome contains a predicted bulged stem-loop (designated P0ab), a conserved cis-acting pseudoknot (PK), and a more distal stem-loop (designated P2). Base-pairing to create the pseudoknot-forming stem (P1(pk)) is mutually exclusive with formation of stem P0a at the base of the bulged stem-loop; as a result, the two structures cannot be present simultaneously. Herein, we use thermodynamic methods to evaluate the ability of individual subdomains of the 3' UTR to adopt a pseudoknotted conformation. We find that an RNA capable of forming only the predicted PK (58 nt; 3' nucleotides 241-185) adopts the P2 stem-loop with little evidence for P1(pk) pairing in 0.1 M KCl and the absence of Mg(2+); as Mg(2+) or 1 M KCl is added, a new thermal unfolding transition is induced and assignable to P1(pk) pairing. The P1(pk) helix is only marginally stable, ΔG(25) ≈ 1.2 ± 0.3 kcal/mol (5.0 mM Mg(2+), 100 mM K(+)), and unfolded at 37°C. Similar findings characterize an RNA 5' extended through the P0b helix only (89 nt; 294-185). In contrast, an RNA capable of forming either the P0a helix or the pseudoknot (97 nt; 301-185) forms no P1(pk) helix. Thermal unfolding simulations are fully consistent with these experimental findings. These data reveal that the PK forms weakly and only when the competing double-hairpin structure cannot form; in the UTR RNA, the double hairpin is the predominant conformer under all solution conditions.</div>
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<Abstract><AbstractText>The 3'-untranslated region (UTR) of the group 2 coronavirus mouse hepatitis virus (MHV) genome contains a predicted bulged stem-loop (designated P0ab), a conserved cis-acting pseudoknot (PK), and a more distal stem-loop (designated P2). Base-pairing to create the pseudoknot-forming stem (P1(pk)) is mutually exclusive with formation of stem P0a at the base of the bulged stem-loop; as a result, the two structures cannot be present simultaneously. Herein, we use thermodynamic methods to evaluate the ability of individual subdomains of the 3' UTR to adopt a pseudoknotted conformation. We find that an RNA capable of forming only the predicted PK (58 nt; 3' nucleotides 241-185) adopts the P2 stem-loop with little evidence for P1(pk) pairing in 0.1 M KCl and the absence of Mg(2+); as Mg(2+) or 1 M KCl is added, a new thermal unfolding transition is induced and assignable to P1(pk) pairing. The P1(pk) helix is only marginally stable, ΔG(25) ≈ 1.2 ± 0.3 kcal/mol (5.0 mM Mg(2+), 100 mM K(+)), and unfolded at 37°C. Similar findings characterize an RNA 5' extended through the P0b helix only (89 nt; 294-185). In contrast, an RNA capable of forming either the P0a helix or the pseudoknot (97 nt; 301-185) forms no P1(pk) helix. Thermal unfolding simulations are fully consistent with these experimental findings. These data reveal that the PK forms weakly and only when the competing double-hairpin structure cannot form; in the UTR RNA, the double hairpin is the predominant conformer under all solution conditions.</AbstractText>
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<ReferenceList><Reference><Citation>J Virol. 1999 Oct;73(10):8349-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10482585</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1999 Jun 25;289(5):1283-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10373368</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2005 Dec;11(12):1884-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16251382</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Am Chem Soc. 2006 Mar 29;128(12):4035-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16551112</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2006 Jun;87(Pt 6):1403-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16690906</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2006;34(9):2634-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16709732</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Virus Res. 2006;66:193-292</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16877062</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2006 Oct 18;25(20):4933-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17024178</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Jan;81(1):20-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16928755</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Feb;81(3):1274-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17093194</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biochem Sci. 2007 Sep;32(9):415-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17764952</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Feb;82(3):1214-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18032506</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2008 Mar 28;377(3):790-803</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18289557</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phys Biol. 2008;5(1):016002</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18367782</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Biophys. 2008;37:197-214</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18573079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2009 Feb;15(2):294-307</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19144910</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Struct Mol Biol. 2009 Mar;16(3):343-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19234468</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2009 Apr;15(4):696-706</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19237463</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2009 Mar 27;33(6):784-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19285444</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chembiochem. 2009 May 4;10(7):1141-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19382115</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2010 Jan;38(1):203-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19875418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Feb;84(3):1423-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19923185</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2010 Mar;16(3):538-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20100813</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2010 Jun 11;399(3):450-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20398674</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA Biol. 2010 May-Jun;7(3):328-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20458165</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2002 Apr;76(8):3697-708</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11907209</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2000 Mar;6(3):409-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10744025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA Biol. 2011 Mar-Apr;8(2):237-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21378501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 Jun 13;300(5626):1763-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12746549</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Jan;78(2):669-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14694098</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Jul;78(14):7846-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15220462</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1985 Mar 11;13(5):1717-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4000943</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 1994 Jan;8(2):221-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8299941</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1994 Apr 15;237(5):560-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7512652</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3373-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8159754</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1995;259:242-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8538457</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1997 Oct;71(10):7567-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9311837</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1998 Apr 17;280(5362):434-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9545221</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1998 Jun 12;279(3):545-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9641977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1998 Oct 8;395(6702):567-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9783582</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2005 Jun 28;44(25):9058-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15966729</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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