SrasV1, Ncbi, Merge, bibRecord, 001F39

Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase.

Identifieur interne : 001F39 ( Ncbi/Merge ); précédent : 001F38; suivant : 001F40

Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase.

Auteurs : Hongwu Wang [République populaire de Chine] ; Meifang Han ; Huaning Yao ; Zhanhui Wang ; Dong Xi ; Weiming Yan ; Jinlin Hou ; Xiaoping Luo ; Qin Ning

Source :

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban [ 1672-0733 ] ; 2009.

RBID : pubmed:19513614

Descripteurs français

English descriptors

KwdEn :
- Animals, CHO Cells, Cricetinae, Cricetulus, Fibrinogen (biosynthesis), Fibrinogen (genetics), Genetic Vectors (genetics), Humans, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Plasmids, Recombinant Proteins (biosynthesis), SARS Virus (genetics), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Thromboplastin (genetics), Thromboplastin (metabolism), Transcription, Genetic (genetics), Transfection, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Matrix Proteins (biosynthesis), Viral Matrix Proteins (genetics).
MESH :
- chemical , biosynthesis : Fibrinogen, Membrane Glycoproteins, Nucleocapsid Proteins, Recombinant Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , genetics : Fibrinogen, Membrane Glycoproteins, Nucleocapsid Proteins, Thromboplastin, Viral Envelope Proteins, Viral Matrix Proteins.
- genetics : Genetic Vectors, SARS Virus, Transcription, Genetic.
- metabolism : SARS Virus, Thromboplastin.
- virology : Severe Acute Respiratory Syndrome.
- Animals, CHO Cells, Cricetinae, Cricetulus, Humans, Plasmids, Spike Glycoprotein, Coronavirus, Transfection.

Abstract

SARS coronavirus (SARS-CoV) is the etiologic agent of severe acute respiratory syndrome. The aim of this study was to construct Sars-CoV membrane (M), nucleocapsid (N) and spike 2 (S2) gene eukaryotic expression plasmids, and identify their expression in vitro. Gene fragments encoding N protein, M protein and S2 protein of SARS-CoV were amplified by PCR using cDNA obtained from lung samples of SARS patients as template, and subcloned into pcDNA3.1 vector to form eukaryotic expression plasmids. SARS-CoV protein eukaryotic expression plasmids were transfected respectively into CHO cells. Immunohistochemistry was employed to detect the expression of the structural proteins of SARS-CoV in transfected cells. SARS-CoV protein eukaryotic expression plasmids were successfully constructed by identification with digestion of restriction enzymes and sequencing. M, N and S2 proteins of SARS-CoV were detected in the cytoplasm of transfected CHO cells. It was concluded that these recombinant eukaryotic expression plasmids were constructed successfully, and SARS-CoV encoding proteins could activate transcription and expression of hfgl2 gene.

DOI: 10.1007/s11596-009-0311-1
PubMed: 19513614

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 001886
to stream PubMed, to step Curation: 001886
to stream PubMed, to step Checkpoint: 001919

Links to Exploration step

pubmed:19513614

Le document en format XML

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<term>Cricetulus</term>
<term>Fibrinogen (biosynthesis)</term>
<term>Fibrinogen (genetics)</term>
<term>Genetic Vectors (genetics)</term>
<term>Humans</term>
<term>Membrane Glycoproteins (biosynthesis)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Nucleocapsid Proteins (biosynthesis)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Plasmids</term>
<term>Recombinant Proteins (biosynthesis)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Thromboplastin (genetics)</term>
<term>Thromboplastin (metabolism)</term>
<term>Transcription, Genetic (genetics)</term>
<term>Transfection</term>
<term>Viral Envelope Proteins (biosynthesis)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Matrix Proteins (biosynthesis)</term>
<term>Viral Matrix Proteins (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules CHO</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Fibrinogène (biosynthèse)</term>
<term>Fibrinogène (génétique)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (biosynthèse)</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Humains</term>
<term>Plasmides</term>
<term>Protéines de l'enveloppe virale (biosynthèse)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de la matrice virale (biosynthèse)</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines nucléocapside (biosynthèse)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines recombinantes (biosynthèse)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Thromboplastine (génétique)</term>
<term>Thromboplastine (métabolisme)</term>
<term>Transcription génétique (génétique)</term>
<term>Transfection</term>
<term>Vecteurs génétiques (génétique)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (métabolisme)</term>
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<term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fibrinogen</term>
<term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Thromboplastin</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Fibrinogène</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genetic Vectors</term>
<term>SARS Virus</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fibrinogène</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
<term>Thromboplastine</term>
<term>Transcription génétique</term>
<term>Vecteurs génétiques</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
<term>Thromboplastin</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Thromboplastine</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>CHO Cells</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Humans</term>
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<term>Spike Glycoprotein, Coronavirus</term>
<term>Transfection</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules CHO</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Plasmides</term>
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<front><div type="abstract" xml:lang="en">SARS coronavirus (SARS-CoV) is the etiologic agent of severe acute respiratory syndrome. The aim of this study was to construct Sars-CoV membrane (M), nucleocapsid (N) and spike 2 (S2) gene eukaryotic expression plasmids, and identify their expression in vitro. Gene fragments encoding N protein, M protein and S2 protein of SARS-CoV were amplified by PCR using cDNA obtained from lung samples of SARS patients as template, and subcloned into pcDNA3.1 vector to form eukaryotic expression plasmids. SARS-CoV protein eukaryotic expression plasmids were transfected respectively into CHO cells. Immunohistochemistry was employed to detect the expression of the structural proteins of SARS-CoV in transfected cells. SARS-CoV protein eukaryotic expression plasmids were successfully constructed by identification with digestion of restriction enzymes and sequencing. M, N and S2 proteins of SARS-CoV were detected in the cytoplasm of transfected CHO cells. It was concluded that these recombinant eukaryotic expression plasmids were constructed successfully, and SARS-CoV encoding proteins could activate transcription and expression of hfgl2 gene.</div>
</front>
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<DateCompleted><Year>2010</Year>
<Month>10</Month>
<Day>21</Day>
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<DateRevised><Year>2020</Year>
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<Title>Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban</Title>
<ISOAbbreviation>J. Huazhong Univ. Sci. Technol. Med. Sci.</ISOAbbreviation>
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<ArticleTitle>Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase.</ArticleTitle>
<Pagination><MedlinePgn>318-23</MedlinePgn>
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<Abstract><AbstractText>SARS coronavirus (SARS-CoV) is the etiologic agent of severe acute respiratory syndrome. The aim of this study was to construct Sars-CoV membrane (M), nucleocapsid (N) and spike 2 (S2) gene eukaryotic expression plasmids, and identify their expression in vitro. Gene fragments encoding N protein, M protein and S2 protein of SARS-CoV were amplified by PCR using cDNA obtained from lung samples of SARS patients as template, and subcloned into pcDNA3.1 vector to form eukaryotic expression plasmids. SARS-CoV protein eukaryotic expression plasmids were transfected respectively into CHO cells. Immunohistochemistry was employed to detect the expression of the structural proteins of SARS-CoV in transfected cells. SARS-CoV protein eukaryotic expression plasmids were successfully constructed by identification with digestion of restriction enzymes and sequencing. M, N and S2 proteins of SARS-CoV were detected in the cytoplasm of transfected CHO cells. It was concluded that these recombinant eukaryotic expression plasmids were constructed successfully, and SARS-CoV encoding proteins could activate transcription and expression of hfgl2 gene.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Hongwu</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Infectious Disease, Huazhong University of Science and Technology, Wuhan, 430030, China. hongwuwang@126.com</Affiliation>
</AffiliationInfo>
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<ForeName>Meifang</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yao</LastName>
<ForeName>Huaning</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Zhanhui</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Xi</LastName>
<ForeName>Dong</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Weiming</ForeName>
<Initials>W</Initials>
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<Author ValidYN="Y"><LastName>Hou</LastName>
<ForeName>Jinlin</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Luo</LastName>
<ForeName>Xiaoping</ForeName>
<Initials>X</Initials>
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<Author ValidYN="Y"><LastName>Ning</LastName>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C095202">FGL2 protein, human</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014763">Viral Matrix Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>108502-71-2</RegistryNumber>
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<Chemical><RegistryNumber>9001-32-5</RegistryNumber>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D016466" MajorTopicYN="N">CHO Cells</DescriptorName>
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<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
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<MeshHeading><DescriptorName UI="D005340" MajorTopicYN="N">Fibrinogen</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
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<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName>
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<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
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<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013925" MajorTopicYN="N">Thromboplastin</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName>
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<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<Month>03</Month>
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<PubMedPubDate PubStatus="medline"><Year>2010</Year>
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<ReferenceList><Reference><Citation>Blood. 2005 Oct 1;106(7):2366-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15860669</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2006 Jul 10;580(16):3829-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16797548</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Exp Med Biol. 1998;440:609-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9782336</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>World J Gastroenterol. 2005 Nov 28;11(44):6936-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16437596</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2006 Jan;80(2):785-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16378980</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Invest. 2003 Jul;112(1):58-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12840059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 Nov;42(11):5309-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15528730</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2003 May 2;278(18):15541-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12594208</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Pathol. 2000 Apr;156(4):1217-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10751347</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem J. 2004 Oct 1;383(Pt 1):13-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15294014</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1999 Apr 9;274(15):9930-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10187767</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2002 May;76(10):5233-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11967337</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genomics Proteomics Bioinformatics. 2003 May;1(2):118-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15626342</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Drug Discov Today. 2003 Sep 1;8(17):768-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12946632</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Biochim Biophys Sin (Shanghai). 2005 Sep;37(9):607-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16143815</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2003 Nov 28;311(4):870-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14623261</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Biochem Cell Biol. 2006;38(8):1417-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16546436</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 1998 Jul 1;161(1):138-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9647217</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Zhonghua Bing Li Xue Za Zhi. 2005 Oct;34(10):656-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16536279</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2005 Jun 1;174(11):7403-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15905589</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
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Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase.

Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase.

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