Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV)
Identifieur interne : 001910 ( Ncbi/Merge ); précédent : 001909; suivant : 001911Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV)
Auteurs : Zhongping He [République populaire de Chine] ; Hui Zhuang [République populaire de Chine] ; Chunhui Zhao [République populaire de Chine] ; Qingming Dong [République populaire de Chine] ; Guoai Peng [République populaire de Chine] ; Dominic E. Dwyer [Australie]Source :
- Virology Journal [ 1743-422X ] ; 2007.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), Adulte, Adulte d'âge moyen, Fèces (virologie), Humains, Pharynx (virologie), Pneumopathie virale (virologie), Pneumopathie virale (épidémiologie), RT-PCR, Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Sérum (virologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : ARN viral.
- génétique : ARN viral.
- isolement et purification : Virus du SRAS.
- virologie : Fèces, Pharynx, Pneumopathie virale, Syndrome respiratoire aigu sévère, Sérum.
- épidémiologie : Pneumopathie virale, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Humains, RT-PCR.
English descriptors
- KwdEn :
- Adult, Feces (virology), Humans, Middle Aged, Pharynx (virology), Pneumonia, Viral (epidemiology), Pneumonia, Viral (virology), RNA, Viral (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (isolation & purification), Serum (virology), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , analysis : RNA, Viral.
- epidemiology : Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- chemical , genetics : RNA, Viral.
- isolation & purification : SARS Virus.
- virology : Feces, Pharynx, Pneumonia, Viral, Serum, Severe Acute Respiratory Syndrome.
- Adult, Humans, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.
SARS-CoV detection rates in sera were highest in the first 9 days of illness, whereas detection was highest in throat washes 5–14 days after onset of symptoms. The highest SARS-CoV RT-PCR rates (70.4–86.3%) and viral loads (log10 4.5–6.1) were seen in fecal samples collected 2–4 weeks after the onset of clinical illness. Fecal samples were frequently SARS-CoV RT-PCR positive beyond 40 days, and occasional sera still had SARS-CoV detected after 3 weeks of illness.
In the context of an extensive outbreak with major pressure on hospital resources, patient self-collected samples are an alternative to nasopharyngeal aspirates for laboratory confirmation of SARS-CoV infection.
Url:
DOI: 10.1186/1743-422X-4-32
PubMed: 17386116
PubMed Central: 1851004
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PMC:1851004Le document en format XML
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<term>Feces (virology)</term>
<term>Humans</term>
<term>Middle Aged</term>
<term>Pharynx (virology)</term>
<term>Pneumonia, Viral (epidemiology)</term>
<term>Pneumonia, Viral (virology)</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (isolation & purification)</term>
<term>Serum (virology)</term>
<term>Severe Acute Respiratory Syndrome (epidemiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
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<term>Adulte d'âge moyen</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Pharynx (virologie)</term>
<term>Pneumopathie virale (virologie)</term>
<term>Pneumopathie virale (épidémiologie)</term>
<term>RT-PCR</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Syndrome respiratoire aigu sévère (épidémiologie)</term>
<term>Sérum (virologie)</term>
<term>Virus du SRAS (isolement et purification)</term>
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</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Pneumonia, Viral</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term>
<term>Pharynx</term>
<term>Pneumopathie virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Sérum</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
<term>Pharynx</term>
<term>Pneumonia, Viral</term>
<term>Serum</term>
<term>Severe Acute Respiratory Syndrome</term>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.</p>
</sec>
<sec><title>Results</title>
<p>SARS-CoV detection rates in sera were highest in the first 9 days of illness, whereas detection was highest in throat washes 5–14 days after onset of symptoms. The highest SARS-CoV RT-PCR rates (70.4–86.3%) and viral loads (log<sub>10</sub>
4.5–6.1) were seen in fecal samples collected 2–4 weeks after the onset of clinical illness. Fecal samples were frequently SARS-CoV RT-PCR positive beyond 40 days, and occasional sera still had SARS-CoV detected after 3 weeks of illness.</p>
</sec>
<sec><title>Conclusion</title>
<p>In the context of an extensive outbreak with major pressure on hospital resources, patient self-collected samples are an alternative to nasopharyngeal aspirates for laboratory confirmation of SARS-CoV infection.</p>
</sec>
</div>
</front>
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Beijing, 100011 China</nlm:aff>
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Beijing, 100054 China</nlm:aff>
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<wicri:regionArea>Beijing</wicri:regionArea>
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<author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name>
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<institution>Department of Microbiology,</institution>
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Beijing, 100083 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
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<author><name sortKey="Zhao, Chunhui" sort="Zhao, Chunhui" uniqKey="Zhao C" first="Chunhui" last="Zhao">Chunhui Zhao</name>
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Beijing, 100054 China</nlm:aff>
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Beijing, 100011 China</nlm:aff>
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<author><name sortKey="Peng, Guoai" sort="Peng, Guoai" uniqKey="Peng G" first="Guoai" last="Peng">Guoai Peng</name>
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<institution>Beijing Ditan Hospital,</institution>
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Beijing, 100011 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
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</author>
<author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E" last="Dwyer">Dominic E. Dwyer</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV)</title>
<author><name sortKey="He, Zhongping" sort="He, Zhongping" uniqKey="He Z" first="Zhongping" last="He">Zhongping He</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.413996.0</institution-id>
<institution>Beijing Ditan Hospital,</institution>
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Beijing, 100011 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.24696.3f</institution-id>
<institution-id institution-id-type="ISNI">000000040369153X</institution-id>
<institution>Capital University of Medical Sciences Affiliated Beijing YouAn Hospital,</institution>
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Beijing, 100054 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.11135.37</institution-id>
<institution-id institution-id-type="ISNI">0000000122569319</institution-id>
<institution>Department of Microbiology,</institution>
<institution>Peking University Health Science Center,</institution>
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Beijing, 100083 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
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</author>
<author><name sortKey="Zhao, Chunhui" sort="Zhao, Chunhui" uniqKey="Zhao C" first="Chunhui" last="Zhao">Chunhui Zhao</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.24696.3f</institution-id>
<institution-id institution-id-type="ISNI">000000040369153X</institution-id>
<institution>Capital University of Medical Sciences Affiliated Beijing YouAn Hospital,</institution>
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Beijing, 100054 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
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<author><name sortKey="Dong, Qingming" sort="Dong, Qingming" uniqKey="Dong Q" first="Qingming" last="Dong">Qingming Dong</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.413996.0</institution-id>
<institution>Beijing Ditan Hospital,</institution>
</institution-wrap>
Beijing, 100011 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Peng, Guoai" sort="Peng, Guoai" uniqKey="Peng G" first="Guoai" last="Peng">Guoai Peng</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.413996.0</institution-id>
<institution>Beijing Ditan Hospital,</institution>
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Beijing, 100011 China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E" last="Dwyer">Dominic E. Dwyer</name>
<affiliation wicri:level="1"><nlm:aff id="Aff4">Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead</wicri:regionArea>
<wicri:noRegion>Westmead</wicri:noRegion>
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</author>
</analytic>
<series><title level="j">Virology Journal</title>
<idno type="eISSN">1743-422X</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.</p>
</sec>
<sec><title>Results</title>
<p>SARS-CoV detection rates in sera were highest in the first 9 days of illness, whereas detection was highest in throat washes 5–14 days after onset of symptoms. The highest SARS-CoV RT-PCR rates (70.4–86.3%) and viral loads (log<sub>10</sub>
4.5–6.1) were seen in fecal samples collected 2–4 weeks after the onset of clinical illness. Fecal samples were frequently SARS-CoV RT-PCR positive beyond 40 days, and occasional sera still had SARS-CoV detected after 3 weeks of illness.</p>
</sec>
<sec><title>Conclusion</title>
<p>In the context of an extensive outbreak with major pressure on hospital resources, patient self-collected samples are an alternative to nasopharyngeal aspirates for laboratory confirmation of SARS-CoV infection.</p>
</sec>
</div>
</front>
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<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).</title>
<author><name sortKey="He, Zhongping" sort="He, Zhongping" uniqKey="He Z" first="Zhongping" last="He">Zhongping He</name>
<affiliation wicri:level="3"><nlm:affiliation>Beijing Ditan Hospital, Beijing, People's Republic of China. zhongpinghe@yahoo.com</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Ditan Hospital, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name>
</author>
<author><name sortKey="Zhao, Chunhui" sort="Zhao, Chunhui" uniqKey="Zhao C" first="Chunhui" last="Zhao">Chunhui Zhao</name>
</author>
<author><name sortKey="Dong, Qingming" sort="Dong, Qingming" uniqKey="Dong Q" first="Qingming" last="Dong">Qingming Dong</name>
</author>
<author><name sortKey="Peng, Guoai" sort="Peng, Guoai" uniqKey="Peng G" first="Guoai" last="Peng">Guoai Peng</name>
</author>
<author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E" last="Dwyer">Dominic E. Dwyer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2007">2007</date>
<idno type="RBID">pubmed:17386116</idno>
<idno type="pmid">17386116</idno>
<idno type="doi">10.1186/1743-422X-4-32</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).</title>
<author><name sortKey="He, Zhongping" sort="He, Zhongping" uniqKey="He Z" first="Zhongping" last="He">Zhongping He</name>
<affiliation wicri:level="3"><nlm:affiliation>Beijing Ditan Hospital, Beijing, People's Republic of China. zhongpinghe@yahoo.com</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Ditan Hospital, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name>
</author>
<author><name sortKey="Zhao, Chunhui" sort="Zhao, Chunhui" uniqKey="Zhao C" first="Chunhui" last="Zhao">Chunhui Zhao</name>
</author>
<author><name sortKey="Dong, Qingming" sort="Dong, Qingming" uniqKey="Dong Q" first="Qingming" last="Dong">Qingming Dong</name>
</author>
<author><name sortKey="Peng, Guoai" sort="Peng, Guoai" uniqKey="Peng G" first="Guoai" last="Peng">Guoai Peng</name>
</author>
<author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E" last="Dwyer">Dominic E. Dwyer</name>
</author>
</analytic>
<series><title level="j">Virology journal</title>
<idno type="eISSN">1743-422X</idno>
<imprint><date when="2007" type="published">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term>
<term>Feces (virology)</term>
<term>Humans</term>
<term>Middle Aged</term>
<term>Pharynx (virology)</term>
<term>Pneumonia, Viral (epidemiology)</term>
<term>Pneumonia, Viral (virology)</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (isolation & purification)</term>
<term>Serum (virology)</term>
<term>Severe Acute Respiratory Syndrome (epidemiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>ARN viral (génétique)</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Pharynx (virologie)</term>
<term>Pneumopathie virale (virologie)</term>
<term>Pneumopathie virale (épidémiologie)</term>
<term>RT-PCR</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Syndrome respiratoire aigu sévère (épidémiologie)</term>
<term>Sérum (virologie)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Pneumonia, Viral</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term>
<term>Pharynx</term>
<term>Pneumopathie virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Sérum</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
<term>Pharynx</term>
<term>Pneumonia, Viral</term>
<term>Serum</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Pneumopathie virale</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adult</term>
<term>Humans</term>
<term>Middle Aged</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Humains</term>
<term>RT-PCR</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.</div>
</front>
</TEI>
</pubmed>
</double>
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