Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays.
Identifieur interne : 001810 ( Ncbi/Merge ); précédent : 001809; suivant : 001811Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays.
Auteurs : Rebecca L. Stoermer [États-Unis] ; Kristin B. Cederquist ; Sean K. Mcfarland ; Michael Y. Sha ; Sharron G. Penn ; Christine D. KeatingSource :
- Journal of the American Chemical Society [ 0002-7863 ] ; 2006.
Descripteurs français
- KwdFr :
- ADN viral (analyse), Argent (), Chlorure de sodium (), Concentration osmolaire, Hepacivirus (), Humains, Métaux (), Nanostructures (), Or (), Propriétés de surface, Sensibilité et spécificité, Sondes moléculaires (), Techniques de sonde moléculaire (instrumentation), Thermodynamique, VIH (Virus de l'Immunodéficience Humaine) (), Virus du SRAS ().
- MESH :
- analyse : ADN viral.
- Argent, Chlorure de sodium, Concentration osmolaire, Hepacivirus, Humains, Métaux, Nanostructures, Or, Propriétés de surface, Sensibilité et spécificité, Sondes moléculaires, Techniques de sonde moléculaire, Thermodynamique, VIH (Virus de l'Immunodéficience Humaine), Virus du SRAS.
English descriptors
- KwdEn :
- DNA, Viral (analysis), Gold (chemistry), HIV (chemistry), Hepacivirus (chemistry), Humans, Metals (chemistry), Molecular Probe Techniques (instrumentation), Molecular Probes (chemistry), Nanostructures (chemistry), Osmolar Concentration, SARS Virus (chemistry), Sensitivity and Specificity, Silver (chemistry), Sodium Chloride (chemistry), Surface Properties, Thermodynamics.
- MESH :
- chemical , analysis : DNA, Viral.
- chemical , chemistry : Gold, Metals, Molecular Probes, Silver, Sodium Chloride.
- chemistry : HIV, Hepacivirus, Nanostructures, SARS Virus.
- instrumentation : Molecular Probe Techniques.
- Humans, Osmolar Concentration, Sensitivity and Specificity, Surface Properties, Thermodynamics.
Abstract
We have combined molecular beacon (MB) probes with barcoded metal nanowires to enable no-wash, sealed chamber, multiplexed detection of nucleic acids. Probe design and experimental parameters important in nanowire-based MB assays are discussed. Loop regions of 24 bases and 5 base pair stem regions in the beacon probes gave optimal performance. Our results suggest that thermodynamic predictions for secondary structure stability of solution-phase MB can guide probe design for nanowire-based assays. Dengue virus-specific probes with predicted solution-phase DeltaG of folding in 500 mM buffered NaCl of approximately -4 kcal/mol performed better than those with DeltaG > -2 or < -6 kcal/mol. Buffered 300-500 mM NaCl was selected after comparison of several buffers previously reported for similar types of assays, and 200-500 mM NaCl was found to be the optimal ionic strength for the hybridization temperatures (25 and 50 degrees C) and probe designs used here. Target binding to the surface as a function of solution concentration fit a Sips isotherm with Kd = 1.7 +/- 0.3 nM. The detection limit was approximately 100 pM, limited by incomplete quenching. Single base mismatches could be discriminated from fully complementary targets. Oligonucleotide target sequences specific for human immunodeficiency, hepatitis C, and severe acute respiratory viruses were assayed simultaneously in a no-wash, sealed chamber, multiplexed experiment in which each of three probe sequences was attached to a different pattern of encoded nanowires. Finally, we demonstrated that probe-coated nanowires retain their selectivity and sensitivity in a triplexed assay after storage for over 3 months.
DOI: 10.1021/ja0658261
PubMed: 17177440
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pubmed:17177440Le document en format XML
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Keating</name></author></analytic><series><title level="j">Journal of the American Chemical Society</title><idno type="ISSN">0002-7863</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Viral (analysis)</term><term>Gold (chemistry)</term><term>HIV (chemistry)</term><term>Hepacivirus (chemistry)</term><term>Humans</term><term>Metals (chemistry)</term><term>Molecular Probe Techniques (instrumentation)</term><term>Molecular Probes (chemistry)</term><term>Nanostructures (chemistry)</term><term>Osmolar Concentration</term><term>SARS Virus (chemistry)</term><term>Sensitivity and Specificity</term><term>Silver (chemistry)</term><term>Sodium Chloride (chemistry)</term><term>Surface Properties</term><term>Thermodynamics</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term><term>Argent ()</term><term>Chlorure de sodium ()</term><term>Concentration osmolaire</term><term>Hepacivirus ()</term><term>Humains</term><term>Métaux ()</term><term>Nanostructures ()</term><term>Or ()</term><term>Propriétés de surface</term><term>Sensibilité et spécificité</term><term>Sondes moléculaires ()</term><term>Techniques de sonde moléculaire (instrumentation)</term><term>Thermodynamique</term><term>VIH (Virus de l'Immunodéficience Humaine) ()</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gold</term><term>Metals</term><term>Molecular Probes</term><term>Silver</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>HIV</term><term>Hepacivirus</term><term>Nanostructures</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Molecular Probe Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Osmolar Concentration</term><term>Sensitivity and Specificity</term><term>Surface Properties</term><term>Thermodynamics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Argent</term><term>Chlorure de sodium</term><term>Concentration osmolaire</term><term>Hepacivirus</term><term>Humains</term><term>Métaux</term><term>Nanostructures</term><term>Or</term><term>Propriétés de surface</term><term>Sensibilité et spécificité</term><term>Sondes moléculaires</term><term>Techniques de sonde moléculaire</term><term>Thermodynamique</term><term>VIH (Virus de l'Immunodéficience Humaine)</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have combined molecular beacon (MB) probes with barcoded metal nanowires to enable no-wash, sealed chamber, multiplexed detection of nucleic acids. Probe design and experimental parameters important in nanowire-based MB assays are discussed. Loop regions of 24 bases and 5 base pair stem regions in the beacon probes gave optimal performance. Our results suggest that thermodynamic predictions for secondary structure stability of solution-phase MB can guide probe design for nanowire-based assays. Dengue virus-specific probes with predicted solution-phase DeltaG of folding in 500 mM buffered NaCl of approximately -4 kcal/mol performed better than those with DeltaG > -2 or < -6 kcal/mol. Buffered 300-500 mM NaCl was selected after comparison of several buffers previously reported for similar types of assays, and 200-500 mM NaCl was found to be the optimal ionic strength for the hybridization temperatures (25 and 50 degrees C) and probe designs used here. Target binding to the surface as a function of solution concentration fit a Sips isotherm with Kd = 1.7 +/- 0.3 nM. The detection limit was approximately 100 pM, limited by incomplete quenching. Single base mismatches could be discriminated from fully complementary targets. Oligonucleotide target sequences specific for human immunodeficiency, hepatitis C, and severe acute respiratory viruses were assayed simultaneously in a no-wash, sealed chamber, multiplexed experiment in which each of three probe sequences was attached to a different pattern of encoded nanowires. Finally, we demonstrated that probe-coated nanowires retain their selectivity and sensitivity in a triplexed assay after storage for over 3 months.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17177440</PMID><DateCompleted><Year>2007</Year><Month>02</Month><Day>20</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0002-7863</ISSN><JournalIssue CitedMedium="Print"><Volume>128</Volume><Issue>51</Issue><PubDate><Year>2006</Year><Month>Dec</Month><Day>27</Day></PubDate></JournalIssue><Title>Journal of the American Chemical Society</Title><ISOAbbreviation>J. Am. Chem. Soc.</ISOAbbreviation></Journal><ArticleTitle>Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays.</ArticleTitle><Pagination><MedlinePgn>16892-903</MedlinePgn></Pagination><Abstract><AbstractText>We have combined molecular beacon (MB) probes with barcoded metal nanowires to enable no-wash, sealed chamber, multiplexed detection of nucleic acids. Probe design and experimental parameters important in nanowire-based MB assays are discussed. Loop regions of 24 bases and 5 base pair stem regions in the beacon probes gave optimal performance. Our results suggest that thermodynamic predictions for secondary structure stability of solution-phase MB can guide probe design for nanowire-based assays. Dengue virus-specific probes with predicted solution-phase DeltaG of folding in 500 mM buffered NaCl of approximately -4 kcal/mol performed better than those with DeltaG > -2 or < -6 kcal/mol. Buffered 300-500 mM NaCl was selected after comparison of several buffers previously reported for similar types of assays, and 200-500 mM NaCl was found to be the optimal ionic strength for the hybridization temperatures (25 and 50 degrees C) and probe designs used here. Target binding to the surface as a function of solution concentration fit a Sips isotherm with Kd = 1.7 +/- 0.3 nM. The detection limit was approximately 100 pM, limited by incomplete quenching. Single base mismatches could be discriminated from fully complementary targets. Oligonucleotide target sequences specific for human immunodeficiency, hepatitis C, and severe acute respiratory viruses were assayed simultaneously in a no-wash, sealed chamber, multiplexed experiment in which each of three probe sequences was attached to a different pattern of encoded nanowires. Finally, we demonstrated that probe-coated nanowires retain their selectivity and sensitivity in a triplexed assay after storage for over 3 months.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stoermer</LastName><ForeName>Rebecca L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cederquist</LastName><ForeName>Kristin B</ForeName><Initials>KB</Initials></Author><Author ValidYN="Y"><LastName>McFarland</LastName><ForeName>Sean K</ForeName><Initials>SK</Initials></Author><Author ValidYN="Y"><LastName>Sha</LastName><ForeName>Michael Y</ForeName><Initials>MY</Initials></Author><Author ValidYN="Y"><LastName>Penn</LastName><ForeName>Sharron G</ForeName><Initials>SG</Initials></Author><Author ValidYN="Y"><LastName>Keating</LastName><ForeName>Christine 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IdType="pubmed">17177440</ArticleId><ArticleId IdType="doi">10.1021/ja0658261</ArticleId><ArticleId IdType="pmc">PMC2849162</ArticleId><ArticleId IdType="mid">NIHMS179577</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 2001 Oct 5;294(5540):137-41</Citation><ArticleIdList><ArticleId IdType="pubmed">11588257</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 1999 Jan;21(1 Suppl):5-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9915493</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2000 Jan;18(1):91-4</Citation><ArticleIdList><ArticleId IdType="pubmed">10625399</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 1999 Nov 15;71(22):5054-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10575961</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 2000 Jul 15;283(1):56-63</Citation><ArticleIdList><ArticleId IdType="pubmed">10929808</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):463-71</Citation><ArticleIdList><ArticleId IdType="pubmed">11846616</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 May 25;96(11):6394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10339598</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2005 Sep;43(9):4585-91</Citation><ArticleIdList><ArticleId IdType="pubmed">16145111</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Nov 1;30(21):e122</Citation><ArticleIdList><ArticleId IdType="pubmed">12409481</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Spectrosc. 2004 Sep;58(9):269A-280A</Citation><ArticleIdList><ArticleId IdType="pubmed">15479516</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2005 Mar;23(3):143-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15734557</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2000 Nov 15;72(22):5535-41</Citation><ArticleIdList><ArticleId IdType="pubmed">11101228</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2002 Jun 26;124(25):7314-23</Citation><ArticleIdList><ArticleId IdType="pubmed">12071740</ArticleId></ArticleIdList></Reference><Reference><Citation>Analyst. 2005 Jul;130(7):1002-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15965520</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genomics Hum Genet. 2001;2:235-58</Citation><ArticleIdList><ArticleId IdType="pubmed">11701650</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13308-13</Citation><ArticleIdList><ArticleId IdType="pubmed">14583593</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1990 Aug 3;249(4968):505-10</Citation><ArticleIdList><ArticleId IdType="pubmed">2200121</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1996 Mar;14(3):303-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9630890</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2002 Dec 11;124(49):14601-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12465970</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 May 25;96(11):6171-6</Citation><ArticleIdList><ArticleId IdType="pubmed">10339560</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2001 Apr;19(4):365-70</Citation><ArticleIdList><ArticleId IdType="pubmed">11283596</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2000 Nov;18(11):1191-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11062440</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2000 Dec 1;72(23):747A-753A</Citation><ArticleIdList><ArticleId IdType="pubmed">11128959</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 Sep 26;301(5641):1884-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14512622</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Mar 15;30(6):1292-305</Citation><ArticleIdList><ArticleId IdType="pubmed">11884626</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1998 Jan;16(1):49-53</Citation><ArticleIdList><ArticleId IdType="pubmed">9447593</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2002 Aug 14;124(32):9606-12</Citation><ArticleIdList><ArticleId IdType="pubmed">12167056</ArticleId></ArticleIdList></Reference><Reference><Citation>Biophys J. 2000 Aug;79(2):975-81</Citation><ArticleIdList><ArticleId IdType="pubmed">10920027</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11538-43</Citation><ArticleIdList><ArticleId IdType="pubmed">9751701</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 2004 Aug 15;331(2):216-23</Citation><ArticleIdList><ArticleId IdType="pubmed">15265725</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Jun 15;30(12):e61</Citation><ArticleIdList><ArticleId IdType="pubmed">12060699</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 2003 Nov 15;322(2):148-55</Citation><ArticleIdList><ArticleId IdType="pubmed">14596821</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Clin Biochem. 2002 Mar;39(Pt 2):114-29</Citation><ArticleIdList><ArticleId IdType="pubmed">11928759</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Rev. 2005 Apr;105(4):1547-62</Citation><ArticleIdList><ArticleId IdType="pubmed">15826019</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chem Lab Med. 2003 Apr;41(4):468-74</Citation><ArticleIdList><ArticleId IdType="pubmed">12747588</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2001 Jan 1;73(1):1-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11195491</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2005 Jun 1;127(21):7932-40</Citation><ArticleIdList><ArticleId IdType="pubmed">15913384</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2003 Apr 1;31(7):1962-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12655013</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2003 Apr 9;125(14):4012-3</Citation><ArticleIdList><ArticleId IdType="pubmed">12670198</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2002 Jun;20(6):249-56</Citation><ArticleIdList><ArticleId IdType="pubmed">12007493</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1997 Mar 15;25(6):1155-61</Citation><ArticleIdList><ArticleId IdType="pubmed">9092624</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2001 Feb 1;29(3):E13</Citation><ArticleIdList><ArticleId IdType="pubmed">11160915</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2001 Feb 1;73(3):471-80</Citation><ArticleIdList><ArticleId IdType="pubmed">11217749</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Chem Biol. 2004 Oct;8(5):547-53</Citation><ArticleIdList><ArticleId IdType="pubmed">15450499</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1996 Aug 15;382(6592):607-9</Citation><ArticleIdList><ArticleId IdType="pubmed">8757129</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14036-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15381774</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2002 Feb 22;295(5559):1503-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11859188</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2004 Feb;22(2):55-8</Citation><ArticleIdList><ArticleId IdType="pubmed">14757035</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2002 May 15;74(10):2240-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12038747</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 1999 Aug 1;71(15):3101-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21662902</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2003 Jul 1;31(13):3406-15</Citation><ArticleIdList><ArticleId IdType="pubmed">12824337</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Mol Med. 2002 Jun;8(6):257-60</Citation><ArticleIdList><ArticleId IdType="pubmed">12067606</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2006 Oct 11;128(40):13243-54</Citation><ArticleIdList><ArticleId IdType="pubmed">17017805</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Chem Biol. 2006 Jun;10(3):272-81</Citation><ArticleIdList><ArticleId IdType="pubmed">16678470</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1990 Aug 30;346(6287):818-22</Citation><ArticleIdList><ArticleId IdType="pubmed">1697402</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><noCountry><name sortKey="Cederquist, Kristin B" sort="Cederquist, Kristin B" uniqKey="Cederquist K" first="Kristin B" last="Cederquist">Kristin B. Cederquist</name><name sortKey="Keating, Christine D" sort="Keating, Christine D" uniqKey="Keating C" first="Christine D" last="Keating">Christine D. Keating</name><name sortKey="Mcfarland, Sean K" sort="Mcfarland, Sean K" uniqKey="Mcfarland S" first="Sean K" last="Mcfarland">Sean K. Mcfarland</name><name sortKey="Penn, Sharron G" sort="Penn, Sharron G" uniqKey="Penn S" first="Sharron G" last="Penn">Sharron G. Penn</name><name sortKey="Sha, Michael Y" sort="Sha, Michael Y" uniqKey="Sha M" first="Michael Y" last="Sha">Michael Y. Sha</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Stoermer, Rebecca L" sort="Stoermer, Rebecca L" uniqKey="Stoermer R" first="Rebecca L" last="Stoermer">Rebecca L. Stoermer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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