Protection of mammalian cells from severe acute respiratory syndrome coronavirus infection by equine neutralizing antibody.
Identifieur interne : 001151 ( Ncbi/Merge ); précédent : 001150; suivant : 001152Protection of mammalian cells from severe acute respiratory syndrome coronavirus infection by equine neutralizing antibody.
Auteurs : Xiliang Wang [République populaire de Chine] ; Bing Ni ; Xinan Du ; Guangyu Zhao ; Wenda Gao ; Xinfu Shi ; Songle Zhang ; Liangyan Zhang ; Dong Wang ; Deyan Luo ; Li Xing ; Haiyan Jiang ; Wanling Li ; Man Jiang ; Liwei Mao ; Yangdong He ; Yu Xiao ; Yuzhang WuSource :
- Antiviral therapy [ 1359-6535 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (pharmacologie), Anticorps antiviraux (toxicité), Anticorps antiviraux (usage thérapeutique), Cellules Vero, Effet cytopathogène viral (), Equus caballus, Femelle, Fragments Fab d'immunoglobuline (administration et posologie), Fragments Fab d'immunoglobuline (immunologie), Fragments Fab d'immunoglobuline (toxicité), Fragments Fab d'immunoglobuline (usage thérapeutique), Immunisation passive, Injections péritoneales, Modèles animaux de maladie humaine, Relation dose-effet des médicaments, Souris, Spécificité des anticorps, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Tests de neutralisation, Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Fragments Fab d'immunoglobuline.
- immunologie : Anticorps antiviraux, Fragments Fab d'immunoglobuline, Syndrome respiratoire aigu sévère, Virus du SRAS.
- pharmacologie : Anticorps antiviraux.
- toxicité : Anticorps antiviraux, Fragments Fab d'immunoglobuline.
- usage thérapeutique : Anticorps antiviraux, Fragments Fab d'immunoglobuline.
- Animaux, Cellules Vero, Effet cytopathogène viral, Equus caballus, Femelle, Immunisation passive, Injections péritoneales, Modèles animaux de maladie humaine, Relation dose-effet des médicaments, Souris, Spécificité des anticorps, Syndrome respiratoire aigu sévère, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Antibodies, Viral (pharmacology), Antibodies, Viral (therapeutic use), Antibodies, Viral (toxicity), Antibody Specificity, Chlorocebus aethiops, Cytopathogenic Effect, Viral (drug effects), Disease Models, Animal, Dose-Response Relationship, Drug, Female, Horses, Immunization, Passive, Immunoglobulin Fab Fragments (administration & dosage), Immunoglobulin Fab Fragments (immunology), Immunoglobulin Fab Fragments (therapeutic use), Immunoglobulin Fab Fragments (toxicity), Injections, Intraperitoneal, Mice, Neutralization Tests, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (therapy), Vero Cells.
- MESH :
- chemical , administration & dosage : Immunoglobulin Fab Fragments.
- chemical , immunology : Antibodies, Viral, Immunoglobulin Fab Fragments.
- chemical , pharmacology : Antibodies, Viral.
- chemical , therapeutic use : Antibodies, Viral, Immunoglobulin Fab Fragments.
- chemical , toxicity : Antibodies, Viral, Immunoglobulin Fab Fragments.
- drug effects : Cytopathogenic Effect, Viral.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- therapy : Severe Acute Respiratory Syndrome.
- Animals, Antibody Specificity, Chlorocebus aethiops, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Horses, Immunization, Passive, Injections, Intraperitoneal, Mice, Neutralization Tests, Vero Cells.
Abstract
The aetiological agent for severe acute respiratory syndrome (SARS) has been determined to be a new type of coronavirus (SARS-CoV) that infects a wide range of mammalian hosts. Up to now, there have been no specific drugs to protect against SARS-CoV infection, thus developing effective strategies against this newly emerged viral infection warrants urgent efforts. Adoptive immune therapy with pathogen-specific heterologous immunoglobulin has been successfully used to control the dissemination of many viral infections. To investigate whether a neutralizing antibody against SARS-CoV raised in an artiodactylous host can have a protective role on primate cells, we prepared serum IgGs and their pepsin-digested F(ab')2 fragments from horses inoculated with purified SARS-CoV (BJ-01 strain). The protective effect of the F(ab')2 fragments against SARS-CoV infection was determined in cultured Vero E6 cells by cytopathic effect (CPE), MTT and plaque-forming assays and in a Balb/c mouse model by CPE and quantitative RT-PCR. The results showed the neutralization titres of F(ab')2 from three horses all reached at least 1:1600, and 50 microg of the F(ab')2 fragments could completely neutralize 1x10(4) TCID50- SARS-CoV in vivo. Additionally, we observed that F(ab')2, against BJ-01 strain could also protect cells from infection by the variant GZ-01 strain in vitro and in vivo. Our work has provided experimental support for testing the protective equine immunoglobulin in future large primate or human trials.
PubMed: 16152762
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pubmed:16152762Le document en format XML
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<term>Injections, Intraperitoneal</term>
<term>Mice</term>
<term>Neutralization Tests</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Severe Acute Respiratory Syndrome (therapy)</term>
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<term>Anticorps antiviraux (usage thérapeutique)</term>
<term>Cellules Vero</term>
<term>Effet cytopathogène viral ()</term>
<term>Equus caballus</term>
<term>Femelle</term>
<term>Fragments Fab d'immunoglobuline (administration et posologie)</term>
<term>Fragments Fab d'immunoglobuline (immunologie)</term>
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<term>Dose-Response Relationship, Drug</term>
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<term>Horses</term>
<term>Immunization, Passive</term>
<term>Injections, Intraperitoneal</term>
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<front><div type="abstract" xml:lang="en">The aetiological agent for severe acute respiratory syndrome (SARS) has been determined to be a new type of coronavirus (SARS-CoV) that infects a wide range of mammalian hosts. Up to now, there have been no specific drugs to protect against SARS-CoV infection, thus developing effective strategies against this newly emerged viral infection warrants urgent efforts. Adoptive immune therapy with pathogen-specific heterologous immunoglobulin has been successfully used to control the dissemination of many viral infections. To investigate whether a neutralizing antibody against SARS-CoV raised in an artiodactylous host can have a protective role on primate cells, we prepared serum IgGs and their pepsin-digested F(ab')2 fragments from horses inoculated with purified SARS-CoV (BJ-01 strain). The protective effect of the F(ab')2 fragments against SARS-CoV infection was determined in cultured Vero E6 cells by cytopathic effect (CPE), MTT and plaque-forming assays and in a Balb/c mouse model by CPE and quantitative RT-PCR. The results showed the neutralization titres of F(ab')2 from three horses all reached at least 1:1600, and 50 microg of the F(ab')2 fragments could completely neutralize 1x10(4) TCID50- SARS-CoV in vivo. Additionally, we observed that F(ab')2, against BJ-01 strain could also protect cells from infection by the variant GZ-01 strain in vitro and in vivo. Our work has provided experimental support for testing the protective equine immunoglobulin in future large primate or human trials.</div>
</front>
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<DateCompleted><Year>2005</Year>
<Month>10</Month>
<Day>19</Day>
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<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
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<JournalIssue CitedMedium="Print"><Volume>10</Volume>
<Issue>5</Issue>
<PubDate><Year>2005</Year>
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<Title>Antiviral therapy</Title>
<ISOAbbreviation>Antivir. Ther. (Lond.)</ISOAbbreviation>
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<ArticleTitle>Protection of mammalian cells from severe acute respiratory syndrome coronavirus infection by equine neutralizing antibody.</ArticleTitle>
<Pagination><MedlinePgn>681-90</MedlinePgn>
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<Abstract><AbstractText>The aetiological agent for severe acute respiratory syndrome (SARS) has been determined to be a new type of coronavirus (SARS-CoV) that infects a wide range of mammalian hosts. Up to now, there have been no specific drugs to protect against SARS-CoV infection, thus developing effective strategies against this newly emerged viral infection warrants urgent efforts. Adoptive immune therapy with pathogen-specific heterologous immunoglobulin has been successfully used to control the dissemination of many viral infections. To investigate whether a neutralizing antibody against SARS-CoV raised in an artiodactylous host can have a protective role on primate cells, we prepared serum IgGs and their pepsin-digested F(ab')2 fragments from horses inoculated with purified SARS-CoV (BJ-01 strain). The protective effect of the F(ab')2 fragments against SARS-CoV infection was determined in cultured Vero E6 cells by cytopathic effect (CPE), MTT and plaque-forming assays and in a Balb/c mouse model by CPE and quantitative RT-PCR. The results showed the neutralization titres of F(ab')2 from three horses all reached at least 1:1600, and 50 microg of the F(ab')2 fragments could completely neutralize 1x10(4) TCID50- SARS-CoV in vivo. Additionally, we observed that F(ab')2, against BJ-01 strain could also protect cells from infection by the variant GZ-01 strain in vitro and in vivo. Our work has provided experimental support for testing the protective equine immunoglobulin in future large primate or human trials.</AbstractText>
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<Author ValidYN="Y"><LastName>Zhang</LastName>
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<Author ValidYN="Y"><LastName>Wang</LastName>
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<ForeName>Yangdong</ForeName>
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<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
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<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>9</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>10</Month>
<Day>20</Day>
<Hour>9</Hour>
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<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
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<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
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<tree><noCountry><name sortKey="Du, Xinan" sort="Du, Xinan" uniqKey="Du X" first="Xinan" last="Du">Xinan Du</name>
<name sortKey="Gao, Wenda" sort="Gao, Wenda" uniqKey="Gao W" first="Wenda" last="Gao">Wenda Gao</name>
<name sortKey="He, Yangdong" sort="He, Yangdong" uniqKey="He Y" first="Yangdong" last="He">Yangdong He</name>
<name sortKey="Jiang, Haiyan" sort="Jiang, Haiyan" uniqKey="Jiang H" first="Haiyan" last="Jiang">Haiyan Jiang</name>
<name sortKey="Jiang, Man" sort="Jiang, Man" uniqKey="Jiang M" first="Man" last="Jiang">Man Jiang</name>
<name sortKey="Li, Wanling" sort="Li, Wanling" uniqKey="Li W" first="Wanling" last="Li">Wanling Li</name>
<name sortKey="Luo, Deyan" sort="Luo, Deyan" uniqKey="Luo D" first="Deyan" last="Luo">Deyan Luo</name>
<name sortKey="Mao, Liwei" sort="Mao, Liwei" uniqKey="Mao L" first="Liwei" last="Mao">Liwei Mao</name>
<name sortKey="Ni, Bing" sort="Ni, Bing" uniqKey="Ni B" first="Bing" last="Ni">Bing Ni</name>
<name sortKey="Shi, Xinfu" sort="Shi, Xinfu" uniqKey="Shi X" first="Xinfu" last="Shi">Xinfu Shi</name>
<name sortKey="Wang, Dong" sort="Wang, Dong" uniqKey="Wang D" first="Dong" last="Wang">Dong Wang</name>
<name sortKey="Wu, Yuzhang" sort="Wu, Yuzhang" uniqKey="Wu Y" first="Yuzhang" last="Wu">Yuzhang Wu</name>
<name sortKey="Xiao, Yu" sort="Xiao, Yu" uniqKey="Xiao Y" first="Yu" last="Xiao">Yu Xiao</name>
<name sortKey="Xing, Li" sort="Xing, Li" uniqKey="Xing L" first="Li" last="Xing">Li Xing</name>
<name sortKey="Zhang, Liangyan" sort="Zhang, Liangyan" uniqKey="Zhang L" first="Liangyan" last="Zhang">Liangyan Zhang</name>
<name sortKey="Zhang, Songle" sort="Zhang, Songle" uniqKey="Zhang S" first="Songle" last="Zhang">Songle Zhang</name>
<name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Wang, Xiliang" sort="Wang, Xiliang" uniqKey="Wang X" first="Xiliang" last="Wang">Xiliang Wang</name>
</noRegion>
</country>
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