Detection of severe acute respiratory syndrome coronavirus RNA in plasma during the course of infection.
Identifieur interne : 000D68 ( Ncbi/Merge ); précédent : 000D67; suivant : 000D69Detection of severe acute respiratory syndrome coronavirus RNA in plasma during the course of infection.
Auteurs : Wei-Kung Wang [Taïwan] ; Chi-Tai Fang ; Hui-Ling Chen ; Chao-Fu Yang ; Yee-Chun Chen ; Mei-Ling Chen ; Shey-Ying Chen ; Jyh-Yuan Yang ; Jih-Hui Lin ; Pan-Chyr Yang ; Shan-Chwen ChangSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (sang), Adulte, Adulte d'âge moyen, Femelle, Humains, Mâle, RT-PCR, Sujet âgé, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (physiopathologie), Syndrome respiratoire aigu sévère (virologie), Taïwan, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- physiopathologie : Syndrome respiratoire aigu sévère.
- sang : ARN viral.
- virologie : Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Femelle, Humains, Mâle, RT-PCR, Sujet âgé, Taïwan.
- Wicri :
- geographic : Taïwan.
English descriptors
- KwdEn :
- Adult, Aged, Female, Humans, Male, Middle Aged, RNA, Viral (blood), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (physiopathology), Severe Acute Respiratory Syndrome (virology), Taiwan.
- MESH :
- chemical , blood : RNA, Viral.
- geographic : Taiwan.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- physiopathology : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Adult, Aged, Female, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from six cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.
DOI: 10.1128/JCM.43.2.962-965.2005
PubMed: 15695719
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âgé</term><term>Taïwan</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Taïwan</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from six cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15695719</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>43</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Detection of severe acute respiratory syndrome coronavirus RNA in plasma during the course of infection.</ArticleTitle><Pagination><MedlinePgn>962-5</MedlinePgn></Pagination><Abstract><AbstractText>We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from six cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Wei-Kung</ForeName><Initials>WK</Initials><AffiliationInfo><Affiliation>Institute of Microbiology, College of Medicine, National Taiwan University, Tapei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Chi-Tai</ForeName><Initials>CT</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Hui-Ling</ForeName><Initials>HL</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chao-Fu</ForeName><Initials>CF</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yee-Chun</ForeName><Initials>YC</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Mei-Ling</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shey-Ying</ForeName><Initials>SY</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jyh-Yuan</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Jih-Hui</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Pan-Chyr</ForeName><Initials>PC</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Shan-Chwen</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><CollectiveName>Members of the SARS Research Group of National Taiwan University College of Medicine-National Taiwan University Hospital</CollectiveName></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal 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18;349(25):2431-41</Citation><ArticleIdList><ArticleId IdType="pubmed">14681510</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chang, Shan Chwen" sort="Chang, Shan Chwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><name sortKey="Chen, Hui Ling" sort="Chen, Hui Ling" uniqKey="Chen H" first="Hui-Ling" last="Chen">Hui-Ling Chen</name><name sortKey="Chen, Mei Ling" sort="Chen, Mei Ling" uniqKey="Chen M" first="Mei-Ling" last="Chen">Mei-Ling Chen</name><name sortKey="Chen, Shey Ying" sort="Chen, Shey Ying" uniqKey="Chen S" first="Shey-Ying" last="Chen">Shey-Ying Chen</name><name sortKey="Chen, Yee Chun" sort="Chen, Yee Chun" uniqKey="Chen Y" first="Yee-Chun" last="Chen">Yee-Chun Chen</name><name sortKey="Fang, Chi Tai" sort="Fang, Chi Tai" uniqKey="Fang C" first="Chi-Tai" last="Fang">Chi-Tai Fang</name><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name><name sortKey="Yang, Chao Fu" sort="Yang, Chao Fu" uniqKey="Yang C" first="Chao-Fu" last="Yang">Chao-Fu Yang</name><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><name sortKey="Yang, Pan Chyr" sort="Yang, Pan Chyr" uniqKey="Yang P" first="Pan-Chyr" last="Yang">Pan-Chyr Yang</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Wang, Wei Kung" sort="Wang, Wei Kung" uniqKey="Wang W" first="Wei-Kung" last="Wang">Wei-Kung Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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