Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.
Identifieur interne : 000C92 ( Ncbi/Merge ); précédent : 000C91; suivant : 000C93Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.
Auteurs : Wenbing Chen [République populaire de Chine] ; Shousong Li ; Biying Shao ; Teng Zheng ; Shuxun Jiang ; Xiaorong Huang ; Kaizhen Cai ; Zhideng ZhangSource :
- Genomics, proteomics & bioinformatics [ 1672-0229 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Complementary.
- chemical : DNA Primers.
- genetics : SARS Virus.
- methods : Polymerase Chain Reaction.
- Base Sequence, Cloning, Molecular, Molecular Sequence Data.
Abstract
The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.
DOI: 10.1016/s1672-0229(04)02008-x
PubMed: 15629044
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002973
- to stream PubMed, to step Curation: 002973
- to stream PubMed, to step Checkpoint: 002B45
Links to Exploration step
pubmed:15629044Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.</title>
<author><name sortKey="Chen, Wenbing" sort="Chen, Wenbing" uniqKey="Chen W" first="Wenbing" last="Chen">Wenbing Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China. 621213wbc@163.com</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003</wicri:regionArea>
<placeName><settlement type="city">Fuzhou</settlement>
<region type="province">Fujian</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Shousong" sort="Li, Shousong" uniqKey="Li S" first="Shousong" last="Li">Shousong Li</name>
</author>
<author><name sortKey="Shao, Biying" sort="Shao, Biying" uniqKey="Shao B" first="Biying" last="Shao">Biying Shao</name>
</author>
<author><name sortKey="Zheng, Teng" sort="Zheng, Teng" uniqKey="Zheng T" first="Teng" last="Zheng">Teng Zheng</name>
</author>
<author><name sortKey="Jiang, Shuxun" sort="Jiang, Shuxun" uniqKey="Jiang S" first="Shuxun" last="Jiang">Shuxun Jiang</name>
</author>
<author><name sortKey="Huang, Xiaorong" sort="Huang, Xiaorong" uniqKey="Huang X" first="Xiaorong" last="Huang">Xiaorong Huang</name>
</author>
<author><name sortKey="Cai, Kaizhen" sort="Cai, Kaizhen" uniqKey="Cai K" first="Kaizhen" last="Cai">Kaizhen Cai</name>
</author>
<author><name sortKey="Zhang, Zhideng" sort="Zhang, Zhideng" uniqKey="Zhang Z" first="Zhideng" last="Zhang">Zhideng Zhang</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15629044</idno>
<idno type="pmid">15629044</idno>
<idno type="doi">10.1016/s1672-0229(04)02008-x</idno>
<idno type="wicri:Area/PubMed/Corpus">002973</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002973</idno>
<idno type="wicri:Area/PubMed/Curation">002973</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002973</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002B45</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B45</idno>
<idno type="wicri:Area/Ncbi/Merge">000C92</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.</title>
<author><name sortKey="Chen, Wenbing" sort="Chen, Wenbing" uniqKey="Chen W" first="Wenbing" last="Chen">Wenbing Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China. 621213wbc@163.com</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003</wicri:regionArea>
<placeName><settlement type="city">Fuzhou</settlement>
<region type="province">Fujian</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Shousong" sort="Li, Shousong" uniqKey="Li S" first="Shousong" last="Li">Shousong Li</name>
</author>
<author><name sortKey="Shao, Biying" sort="Shao, Biying" uniqKey="Shao B" first="Biying" last="Shao">Biying Shao</name>
</author>
<author><name sortKey="Zheng, Teng" sort="Zheng, Teng" uniqKey="Zheng T" first="Teng" last="Zheng">Teng Zheng</name>
</author>
<author><name sortKey="Jiang, Shuxun" sort="Jiang, Shuxun" uniqKey="Jiang S" first="Shuxun" last="Jiang">Shuxun Jiang</name>
</author>
<author><name sortKey="Huang, Xiaorong" sort="Huang, Xiaorong" uniqKey="Huang X" first="Xiaorong" last="Huang">Xiaorong Huang</name>
</author>
<author><name sortKey="Cai, Kaizhen" sort="Cai, Kaizhen" uniqKey="Cai K" first="Kaizhen" last="Cai">Kaizhen Cai</name>
</author>
<author><name sortKey="Zhang, Zhideng" sort="Zhang, Zhideng" uniqKey="Zhang Z" first="Zhideng" last="Zhang">Zhideng Zhang</name>
</author>
</analytic>
<series><title level="j">Genomics, proteomics & bioinformatics</title>
<idno type="ISSN">1672-0229</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>DNA Primers</term>
<term>DNA, Complementary (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term>
<term>Amorces ADN</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Séquence nucléotidique</term>
<term>Virus du SRAS (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Molecular Sequence Data</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Séquence nucléotidique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15629044</PMID>
<DateCompleted><Year>2005</Year>
<Month>03</Month>
<Day>30</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>16</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1672-0229</ISSN>
<JournalIssue CitedMedium="Print"><Volume>2</Volume>
<Issue>1</Issue>
<PubDate><Year>2004</Year>
<Month>Feb</Month>
</PubDate>
</JournalIssue>
<Title>Genomics, proteomics & bioinformatics</Title>
<ISOAbbreviation>Genomics Proteomics Bioinformatics</ISOAbbreviation>
</Journal>
<ArticleTitle>Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.</ArticleTitle>
<Pagination><MedlinePgn>55-8</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Wenbing</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China. 621213wbc@163.com</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Shousong</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Shao</LastName>
<ForeName>Biying</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Teng</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Shuxun</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Xiaorong</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Cai</LastName>
<ForeName>Kaizhen</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Zhideng</ForeName>
<Initials>Z</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Genomics Proteomics Bioinformatics</MedlineTA>
<NlmUniqueID>101197608</NlmUniqueID>
<ISSNLinking>1672-0229</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>3</Month>
<Day>31</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15629044</ArticleId>
<ArticleId IdType="pmc">PMC5172431</ArticleId>
<ArticleId IdType="pii">S1672-0229(04)02008-X</ArticleId>
<ArticleId IdType="doi">10.1016/s1672-0229(04)02008-x</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Fujian</li>
</region>
<settlement><li>Fuzhou</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Cai, Kaizhen" sort="Cai, Kaizhen" uniqKey="Cai K" first="Kaizhen" last="Cai">Kaizhen Cai</name>
<name sortKey="Huang, Xiaorong" sort="Huang, Xiaorong" uniqKey="Huang X" first="Xiaorong" last="Huang">Xiaorong Huang</name>
<name sortKey="Jiang, Shuxun" sort="Jiang, Shuxun" uniqKey="Jiang S" first="Shuxun" last="Jiang">Shuxun Jiang</name>
<name sortKey="Li, Shousong" sort="Li, Shousong" uniqKey="Li S" first="Shousong" last="Li">Shousong Li</name>
<name sortKey="Shao, Biying" sort="Shao, Biying" uniqKey="Shao B" first="Biying" last="Shao">Biying Shao</name>
<name sortKey="Zhang, Zhideng" sort="Zhang, Zhideng" uniqKey="Zhang Z" first="Zhideng" last="Zhang">Zhideng Zhang</name>
<name sortKey="Zheng, Teng" sort="Zheng, Teng" uniqKey="Zheng T" first="Teng" last="Zheng">Teng Zheng</name>
</noCountry>
<country name="République populaire de Chine"><region name="Fujian"><name sortKey="Chen, Wenbing" sort="Chen, Wenbing" uniqKey="Chen W" first="Wenbing" last="Chen">Wenbing Chen</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C92 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000C92 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Merge |type= RBID |clé= pubmed:15629044 |texte= Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:15629044" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |