[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological characteristics].
Identifieur interne : 000864 ( Ncbi/Merge ); précédent : 000863; suivant : 000865[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological characteristics].
Auteurs : Xue-Yuan Dong [République populaire de Chine] ; Xiao-Ang Yang ; Yan Li ; Guo-Bin Xu ; Zhen-Ru Feng ; Yue-Dan Wang ; Wei-Feng ChenSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2004.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (analyse), Clonage moléculaire, Escherichia coli (génétique), Escherichia coli (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (isolement et purification), Humains, Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (isolement et purification), Protéines recombinantes (biosynthèse), Protéines recombinantes (isolement et purification), Protéines virales (biosynthèse), Protéines virales (immunologie), Protéines virales (isolement et purification), Régulation de l'expression des gènes viraux, Sous-unités de protéines (biosynthèse), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (sang), Sérum (métabolisme), Thiorédoxines (biosynthèse), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- analyse : Anticorps antiviraux.
- biosynthèse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Protéines virales, Sous-unités de protéines, Thiorédoxines.
- génétique : Escherichia coli, Virus du SRAS.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Protéines virales.
- métabolisme : Escherichia coli, Sérum.
- sang : Syndrome respiratoire aigu sévère.
- Clonage moléculaire, Glycoprotéine de spicule des coronavirus, Humains, Régulation de l'expression des gènes viraux, Virus du SRAS.
English descriptors
- KwdEn :
- Antibodies, Viral (analysis), Cloning, Molecular, Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression Regulation, Viral, Humans, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (immunology), Membrane Glycoproteins (isolation & purification), Protein Subunits (biosynthesis), Recombinant Proteins (biosynthesis), Recombinant Proteins (isolation & purification), SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (immunology), Serum (metabolism), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Thioredoxins (biosynthesis), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (immunology), Viral Envelope Proteins (isolation & purification), Viral Proteins (biosynthesis), Viral Proteins (immunology), Viral Proteins (isolation & purification).
- MESH :
- chemical , analysis : Antibodies, Viral.
- chemical , biosynthesis : Membrane Glycoproteins, Protein Subunits, Recombinant Proteins, Thioredoxins, Viral Envelope Proteins, Viral Proteins.
- blood : Severe Acute Respiratory Syndrome.
- chemistry : SARS Virus.
- genetics : Escherichia coli, SARS Virus.
- chemical , immunology : Membrane Glycoproteins, SARS Virus, Severe Acute Respiratory Syndrome, Viral Envelope Proteins, Viral Proteins.
- chemical , isolation & purification : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins, Viral Proteins.
- metabolism : Escherichia coli, Serum.
- Cloning, Molecular, Gene Expression Regulation, Viral, Humans, Spike Glycoprotein, Coronavirus.
Abstract
To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.
PubMed: 15193211
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pubmed:15193211Le document en format XML
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first="Wei-Feng" last="Chen">Wei-Feng Chen</name></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (analysis)</term><term>Cloning, Molecular</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Gene Expression Regulation, Viral</term><term>Humans</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (isolation & purification)</term><term>Protein Subunits (biosynthesis)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (isolation & purification)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Serum (metabolism)</term><term>Severe Acute Respiratory Syndrome (blood)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Thioredoxins (biosynthesis)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Proteins (biosynthesis)</term><term>Viral Proteins (immunology)</term><term>Viral Proteins (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (analyse)</term><term>Clonage moléculaire</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (isolement et purification)</term><term>Humains</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (isolement et purification)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines virales (biosynthèse)</term><term>Protéines virales (immunologie)</term><term>Protéines virales (isolement et purification)</term><term>Régulation de l'expression des gènes viraux</term><term>Sous-unités de protéines (biosynthèse)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (sang)</term><term>Sérum (métabolisme)</term><term>Thiorédoxines (biosynthèse)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>Thioredoxins</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Sous-unités de protéines</term><term>Thiorédoxines</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Serum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Sérum</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Humans</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Régulation de l'expression des gènes viraux</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15193211</PMID><DateCompleted><Year>2004</Year><Month>12</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>May</Month></PubDate></JournalIssue><Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title><ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological characteristics].</ArticleTitle><Pagination><MedlinePgn>257-60</MedlinePgn></Pagination><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The Trx-S2 fusion protein was expressed in E.coli. After purification, the Trx-S2 fusion protein was detected by Western blot with 6 serum samples of convalescent SARS patients and 6 serum samples of healthy donors.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">According to the SDS-PAGE analysis, the relative molecular mass (M(r)) of the Trx-S2 fusion protein is about 76 x 10(3). The fusion protein could react with all the sera from convalescent SARS patients but not with the sera from healthy donors.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The Trx-S2 fusion protein provides a basis for the research on its role in the course of SARS virus infection of host cells and preparation of recombinant vaccine against SARS virus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Xue-yuan</ForeName><Initials>XY</Initials><AffiliationInfo><Affiliation>Department of Immunology, Health Science Center, Peking University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiao-ang</ForeName><Initials>XA</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Guo-bin</ForeName><Initials>GB</Initials></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Zhen-ru</ForeName><Initials>ZR</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yue-dan</ForeName><Initials>YD</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Wei-feng</ForeName><Initials>WF</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA><NlmUniqueID>101139110</NlmUniqueID><ISSNLinking>1007-8738</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021122">Protein Subunits</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>52500-60-4</RegistryNumber><NameOfSubstance UI="D013879">Thioredoxins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015967" MajorTopicYN="N">Gene Expression Regulation, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021122" MajorTopicYN="N">Protein Subunits</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044967" MajorTopicYN="N">Serum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013879" MajorTopicYN="N">Thioredoxins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>6</Month><Day>15</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>12</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>6</Month><Day>15</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15193211</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><noCountry><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name><name sortKey="Yang, Xiao Ang" sort="Yang, Xiao Ang" uniqKey="Yang X" first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Dong, Xue Yuan" sort="Dong, Xue Yuan" uniqKey="Dong X" first="Xue-Yuan" last="Dong">Xue-Yuan Dong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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