Inducible Bronchus-Associated Lymphoid Tissue Elicited by a Protein Cage Nanoparticle Enhances Protection in Mice against Diverse Respiratory Viruses
Identifieur interne : 002000 ( Ncbi/Curation ); précédent : 001F99; suivant : 002001Inducible Bronchus-Associated Lymphoid Tissue Elicited by a Protein Cage Nanoparticle Enhances Protection in Mice against Diverse Respiratory Viruses
Auteurs : James A. Wiley [États-Unis] ; Laura E. Richert [États-Unis] ; Steve D. Swain [États-Unis] ; Ann Harmsen [États-Unis] ; Dale L. Barnard [États-Unis] ; Troy D. Randall [États-Unis] ; Mark Jutila [États-Unis] ; Trevor Douglas [États-Unis] ; Chris Broomell [États-Unis] ; Mark Young [États-Unis] ; Allen Harmsen [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Bronches (microbiologie), Bronches (métabolisme), Bronches (virologie), Coxiella burnetii (métabolisme), Femelle, Liquide de lavage bronchoalvéolaire, Mâle, Nanoparticules (), Nanotechnologie (), Orthomyxoviridae (métabolisme), Pneumovirus (métabolisme), Poumon (immunologie), Poumon (microbiologie), Poumon (virologie), Souris, Souris de lignée BALB C, Souris de lignée C3H, Tissu lymphoïde (métabolisme), Virus du SRAS (métabolisme).
- MESH :
- immunologie : Poumon.
- microbiologie : Bronches, Poumon.
- métabolisme : Bronches, Coxiella burnetii, Orthomyxoviridae, Pneumovirus, Tissu lymphoïde, Virus du SRAS.
- virologie : Bronches, Poumon.
- Animaux, Femelle, Liquide de lavage bronchoalvéolaire, Mâle, Nanoparticules, Nanotechnologie, Souris, Souris de lignée BALB C, Souris de lignée C3H.
English descriptors
- KwdEn :
- Animals, Bronchi (metabolism), Bronchi (microbiology), Bronchi (virology), Bronchoalveolar Lavage Fluid, Coxiella burnetii (metabolism), Female, Infections (pathology), Lung (immunology), Lung (microbiology), Lung (virology), Lymphoid Tissue (metabolism), Male, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Nanoparticles (chemistry), Nanotechnology (methods), Orthomyxoviridae (metabolism), Pneumovirus (metabolism), SARS Virus (metabolism).
- MESH :
- chemistry : Nanoparticles.
- immunology : Lung.
- metabolism : Bronchi, Coxiella burnetii, Lymphoid Tissue, Orthomyxoviridae, Pneumovirus, SARS Virus.
- methods : Nanotechnology.
- microbiology : Bronchi, Lung.
- pathology : Infections.
- virology : Bronchi, Lung.
- Animals, Bronchoalveolar Lavage Fluid, Female, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C3H.
Abstract
Destruction of the architectural and subsequently the functional integrity of the lung following pulmonary viral infections is attributable to both the extent of pathogen replication and to the host-generated inflammation associated with the recruitment of immune responses. The presence of antigenically disparate pulmonary viruses and the emergence of novel viruses assures the recurrence of lung damage with infection and resolution of each primary viral infection. Thus, there is a need to develop safe broad spectrum immunoprophylactic strategies capable of enhancing protective immune responses in the lung but which limits immune-mediated lung damage. The immunoprophylactic strategy described here utilizes a protein cage nanoparticle (PCN) to significantly accelerate clearance of diverse respiratory viruses after primary infection and also results in a host immune response that causes less lung damage.
Mice pre-treated with PCN, independent of any specific viral antigens, were protected against both sub-lethal and lethal doses of two different influenza viruses, a mouse-adapted SARS-coronavirus, or mouse pneumovirus. Treatment with PCN significantly increased survival and was marked by enhanced viral clearance, accelerated induction of viral-specific antibody production, and significant decreases in morbidity and lung damage. The enhanced protection appears to be dependent upon the prior development of inducible bronchus-associated lymphoid tissue (iBALT) in the lung in response to the PCN treatment and to be mediated through CD4+ T cell and B cell dependent mechanisms.
The immunoprophylactic strategy described utilizes an infection-independent induction of naturally occurring iBALT prior to infection by a pulmonary viral pathogen. This strategy non-specifically enhances primary immunity to respiratory viruses and is not restricted by the antigen specificities inherent in typical vaccination strategies. PCN treatment is asymptomatic in its application and importantly, ameliorates the damaging inflammation normally associated with the recruitment of immune responses into the lung.
Url:
DOI: 10.1371/journal.pone.0007142
PubMed: 19774076
PubMed Central: 2743193
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PMC:2743193Le document en format XML
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Wiley</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Richert, Laura E" sort="Richert, Laura E" uniqKey="Richert L" first="Laura E." last="Richert">Laura E. 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Barnard</name><affiliation wicri:level="2"><nlm:aff id="aff5"><addr-line>Institute for Antiviral Research, Utah State University, Logan, Utah, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute for Antiviral Research, Utah State University, Logan, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Randall, Troy D" sort="Randall, Troy D" uniqKey="Randall T" first="Troy D." last="Randall">Troy D. Randall</name><affiliation wicri:level="2"><nlm:aff id="aff6"><addr-line>Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Jutila, Mark" sort="Jutila, Mark" uniqKey="Jutila M" first="Mark" last="Jutila">Mark Jutila</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Douglas, Trevor" sort="Douglas, Trevor" uniqKey="Douglas T" first="Trevor" last="Douglas">Trevor Douglas</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Broomell, Chris" sort="Broomell, Chris" uniqKey="Broomell C" first="Chris" last="Broomell">Chris Broomell</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Young, Mark" sort="Young, Mark" uniqKey="Young M" first="Mark" last="Young">Mark Young</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Bio-Inspired Nanomaterials, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Department of Plant Sciences, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Harmsen, Allen" sort="Harmsen, Allen" uniqKey="Harmsen A" first="Allen" last="Harmsen">Allen Harmsen</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bronchi (metabolism)</term><term>Bronchi (microbiology)</term><term>Bronchi (virology)</term><term>Bronchoalveolar Lavage Fluid</term><term>Coxiella burnetii (metabolism)</term><term>Female</term><term>Infections (pathology)</term><term>Lung (immunology)</term><term>Lung (microbiology)</term><term>Lung (virology)</term><term>Lymphoid Tissue (metabolism)</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C3H</term><term>Nanoparticles (chemistry)</term><term>Nanotechnology (methods)</term><term>Orthomyxoviridae (metabolism)</term><term>Pneumovirus (metabolism)</term><term>SARS Virus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bronches (microbiologie)</term><term>Bronches (métabolisme)</term><term>Bronches (virologie)</term><term>Coxiella burnetii (métabolisme)</term><term>Femelle</term><term>Liquide de lavage bronchoalvéolaire</term><term>Mâle</term><term>Nanoparticules ()</term><term>Nanotechnologie ()</term><term>Orthomyxoviridae (métabolisme)</term><term>Pneumovirus (métabolisme)</term><term>Poumon (immunologie)</term><term>Poumon (microbiologie)</term><term>Poumon (virologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C3H</term><term>Tissu lymphoïde (métabolisme)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bronchi</term><term>Coxiella burnetii</term><term>Lymphoid Tissue</term><term>Orthomyxoviridae</term><term>Pneumovirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bronches</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bronchi</term><term>Lung</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bronches</term><term>Coxiella burnetii</term><term>Orthomyxoviridae</term><term>Pneumovirus</term><term>Tissu lymphoïde</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Bronches</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bronchi</term><term>Lung</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bronchoalveolar Lavage Fluid</term><term>Female</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C3H</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Liquide de lavage bronchoalvéolaire</term><term>Mâle</term><term>Nanoparticules</term><term>Nanotechnologie</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C3H</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Destruction of the architectural and subsequently the functional integrity of the lung following pulmonary viral infections is attributable to both the extent of pathogen replication and to the host-generated inflammation associated with the recruitment of immune responses. The presence of antigenically disparate pulmonary viruses and the emergence of novel viruses assures the recurrence of lung damage with infection and resolution of each primary viral infection. Thus, there is a need to develop safe broad spectrum immunoprophylactic strategies capable of enhancing protective immune responses in the lung but which limits immune-mediated lung damage. The immunoprophylactic strategy described here utilizes a protein cage nanoparticle (PCN) to significantly accelerate clearance of diverse respiratory viruses after primary infection and also results in a host immune response that causes less lung damage.</p></sec><sec><title>Methodology/Principal Findings</title><p>Mice pre-treated with PCN, independent of any specific viral antigens, were protected against both sub-lethal and lethal doses of two different influenza viruses, a mouse-adapted SARS-coronavirus, or mouse pneumovirus. Treatment with PCN significantly increased survival and was marked by enhanced viral clearance, accelerated induction of viral-specific antibody production, and significant decreases in morbidity and lung damage. The enhanced protection appears to be dependent upon the prior development of inducible bronchus-associated lymphoid tissue (iBALT) in the lung in response to the PCN treatment and to be mediated through CD4+ T cell and B cell dependent mechanisms.</p></sec><sec><title>Conclusions/Significance</title><p>The immunoprophylactic strategy described utilizes an infection-independent induction of naturally occurring iBALT prior to infection by a pulmonary viral pathogen. This strategy non-specifically enhances primary immunity to respiratory viruses and is not restricted by the antigen specificities inherent in typical vaccination strategies. PCN treatment is asymptomatic in its application and importantly, ameliorates the damaging inflammation normally associated with the recruitment of immune responses into the lung.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Belshe, R" uniqKey="Belshe R">R Belshe</name></author><author><name sortKey="Lee, Ms" uniqKey="Lee M">MS Lee</name></author><author><name sortKey="Walker, Re" uniqKey="Walker R">RE Walker</name></author><author><name sortKey="Stoddard, J" uniqKey="Stoddard J">J Stoddard</name></author><author><name sortKey="Mendelman, Pm" uniqKey="Mendelman P">PM Mendelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Treanor, J" uniqKey="Treanor J">J Treanor</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H Wu</name></author><author><name sortKey="Kiang, H" uniqKey="Kiang H">H Kiang</name></author><author><name sortKey="Topham, Dj" uniqKey="Topham D">DJ 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uniqKey="Wiley J">JA Wiley</name></author><author><name sortKey="Tighe, Mp" uniqKey="Tighe M">MP Tighe</name></author><author><name sortKey="Harmsen, Ag" uniqKey="Harmsen A">AG Harmsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wiley, Ja" uniqKey="Wiley J">JA Wiley</name></author><author><name sortKey="Cerwenka, A" uniqKey="Cerwenka A">A Cerwenka</name></author><author><name sortKey="Harkema, Jr" uniqKey="Harkema J">JR Harkema</name></author><author><name sortKey="Dutton, Rw" uniqKey="Dutton R">RW Dutton</name></author><author><name sortKey="Harmsen, Ag" uniqKey="Harmsen A">AG Harmsen</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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