The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication.
Identifieur interne : 001E91 ( Ncbi/Curation ); précédent : 001E90; suivant : 001E92The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication.
Auteurs : Shaun Tylor [Canada] ; Anton Andonov ; Todd Cutts ; Jingxin Cao ; Elsey Grudesky ; Gary Van Domselaar ; Xuguang Li ; Runtao HeSource :
- Canadian journal of microbiology [ 0008-4166 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Arginine (), Cellules Vero, Données de séquences moléculaires, Humains, Motifs d'acides aminés (génétique), Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Réplication virale, Séquence d'acides aminés, Sérine (), Transfection, Virus du SRAS (génétique), Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- génétique : Motifs d'acides aminés, Protéines nucléocapside, Virus du SRAS.
- métabolisme : Protéines nucléocapside, Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Arginine, Cellules Vero, Données de séquences moléculaires, Humains, Protéines nucléocapside, Réplication virale, Séquence d'acides aminés, Sérine, Transfection.
English descriptors
- KwdEn :
- Amino Acid Motifs (genetics), Amino Acid Sequence, Animals, Arginine (chemistry), Chlorocebus aethiops, Humans, Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), SARS Virus (physiology), Serine (chemistry), Transfection, Vero Cells, Virus Replication.
- MESH :
- chemical , chemistry : Arginine, Nucleocapsid Proteins, Serine.
- genetics : Amino Acid Motifs, Nucleocapsid Proteins, SARS Virus.
- chemical , metabolism : Nucleocapsid Proteins, SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Humans, Molecular Sequence Data, Transfection, Vero Cells, Virus Replication.
Abstract
The multimerization/self-interaction of viral proteins is an important step in the process of viral assembly and maturation. Our previous study indicated that the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid protein forms self-multimers through a serine-arginine (SR)-rich motif (SSRSSSRSRGNSR) by using a mammalian two-hybrid system. To determine the biological relevance of this motif, we constructed a SARS-CoV reverse genetic construct by using a bacterial artificial chromosome (BAC)-based vector controlled by a T7 promoter; and subsequently deleted the SR-rich motif from the N gene. The mutated infectious clone showed reduced level of genome transcription and significantly reduced levels of the infectious virions. These results strongly suggest that the SR-rich motif is critical for effective virus replication.
DOI: 10.1139/w08-139
PubMed: 19370068
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001926
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001926
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001765
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001E91
Links to Exploration step
pubmed:19370068Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication.</title>
<author><name sortKey="Tylor, Shaun" sort="Tylor, Shaun" uniqKey="Tylor S" first="Shaun" last="Tylor">Shaun Tylor</name>
<affiliation wicri:level="1"><nlm:affiliation>National Microbiology Laboratory, Health Canada, 1015 Arlington St, Winnipeg, MB R3E3R2, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>National Microbiology Laboratory, Health Canada, 1015 Arlington St, Winnipeg, MB R3E3R2</wicri:regionArea>
<wicri:noRegion>MB R3E3R2</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Andonov, Anton" sort="Andonov, Anton" uniqKey="Andonov A" first="Anton" last="Andonov">Anton Andonov</name>
</author>
<author><name sortKey="Cutts, Todd" sort="Cutts, Todd" uniqKey="Cutts T" first="Todd" last="Cutts">Todd Cutts</name>
</author>
<author><name sortKey="Cao, Jingxin" sort="Cao, Jingxin" uniqKey="Cao J" first="Jingxin" last="Cao">Jingxin Cao</name>
</author>
<author><name sortKey="Grudesky, Elsey" sort="Grudesky, Elsey" uniqKey="Grudesky E" first="Elsey" last="Grudesky">Elsey Grudesky</name>
</author>
<author><name sortKey="Van Domselaar, Gary" sort="Van Domselaar, Gary" uniqKey="Van Domselaar G" first="Gary" last="Van Domselaar">Gary Van Domselaar</name>
</author>
<author><name sortKey="Li, Xuguang" sort="Li, Xuguang" uniqKey="Li X" first="Xuguang" last="Li">Xuguang Li</name>
</author>
<author><name sortKey="He, Runtao" sort="He, Runtao" uniqKey="He R" first="Runtao" last="He">Runtao He</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19370068</idno>
<idno type="pmid">19370068</idno>
<idno type="doi">10.1139/w08-139</idno>
<idno type="wicri:Area/PubMed/Corpus">001926</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001926</idno>
<idno type="wicri:Area/PubMed/Curation">001926</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001926</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001765</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001765</idno>
<idno type="wicri:Area/Ncbi/Merge">001E91</idno>
<idno type="wicri:Area/Ncbi/Curation">001E91</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication.</title>
<author><name sortKey="Tylor, Shaun" sort="Tylor, Shaun" uniqKey="Tylor S" first="Shaun" last="Tylor">Shaun Tylor</name>
<affiliation wicri:level="1"><nlm:affiliation>National Microbiology Laboratory, Health Canada, 1015 Arlington St, Winnipeg, MB R3E3R2, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>National Microbiology Laboratory, Health Canada, 1015 Arlington St, Winnipeg, MB R3E3R2</wicri:regionArea>
<wicri:noRegion>MB R3E3R2</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Andonov, Anton" sort="Andonov, Anton" uniqKey="Andonov A" first="Anton" last="Andonov">Anton Andonov</name>
</author>
<author><name sortKey="Cutts, Todd" sort="Cutts, Todd" uniqKey="Cutts T" first="Todd" last="Cutts">Todd Cutts</name>
</author>
<author><name sortKey="Cao, Jingxin" sort="Cao, Jingxin" uniqKey="Cao J" first="Jingxin" last="Cao">Jingxin Cao</name>
</author>
<author><name sortKey="Grudesky, Elsey" sort="Grudesky, Elsey" uniqKey="Grudesky E" first="Elsey" last="Grudesky">Elsey Grudesky</name>
</author>
<author><name sortKey="Van Domselaar, Gary" sort="Van Domselaar, Gary" uniqKey="Van Domselaar G" first="Gary" last="Van Domselaar">Gary Van Domselaar</name>
</author>
<author><name sortKey="Li, Xuguang" sort="Li, Xuguang" uniqKey="Li X" first="Xuguang" last="Li">Xuguang Li</name>
</author>
<author><name sortKey="He, Runtao" sort="He, Runtao" uniqKey="He R" first="Runtao" last="He">Runtao He</name>
</author>
</analytic>
<series><title level="j">Canadian journal of microbiology</title>
<idno type="ISSN">0008-4166</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (genetics)</term>
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Arginine (chemistry)</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>SARS Virus (physiology)</term>
<term>Serine (chemistry)</term>
<term>Transfection</term>
<term>Vero Cells</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Arginine ()</term>
<term>Cellules Vero</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Motifs d'acides aminés (génétique)</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Réplication virale</term>
<term>Séquence d'acides aminés</term>
<term>Sérine ()</term>
<term>Transfection</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (métabolisme)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arginine</term>
<term>Nucleocapsid Proteins</term>
<term>Serine</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Nucleocapsid Proteins</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Motifs d'acides aminés</term>
<term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Transfection</term>
<term>Vero Cells</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Arginine</term>
<term>Cellules Vero</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Protéines nucléocapside</term>
<term>Réplication virale</term>
<term>Séquence d'acides aminés</term>
<term>Sérine</term>
<term>Transfection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The multimerization/self-interaction of viral proteins is an important step in the process of viral assembly and maturation. Our previous study indicated that the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid protein forms self-multimers through a serine-arginine (SR)-rich motif (SSRSSSRSRGNSR) by using a mammalian two-hybrid system. To determine the biological relevance of this motif, we constructed a SARS-CoV reverse genetic construct by using a bacterial artificial chromosome (BAC)-based vector controlled by a T7 promoter; and subsequently deleted the SR-rich motif from the N gene. The mutated infectious clone showed reduced level of genome transcription and significantly reduced levels of the infectious virions. These results strongly suggest that the SR-rich motif is critical for effective virus replication.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E91 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001E91 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:19370068 |texte= The SR-rich motif in SARS-CoV nucleocapsid protein is important for virus replication. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:19370068" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |