Concentration and detection of SARS coronavirus in sewage from Xiao Tang Shan Hospital and the 309th Hospital.
Identifieur interne : 001014 ( Ncbi/Curation ); précédent : 001013; suivant : 001015Concentration and detection of SARS coronavirus in sewage from Xiao Tang Shan Hospital and the 309th Hospital.
Auteurs : Xin-Wei Wang [République populaire de Chine] ; Jin-Song Li ; Ting-Kai Guo ; Bei Zhen ; Qing-Xin Kong ; Bin Yi ; Zhong Li ; Nong Song ; Min Jin ; Wen-Jun Xiao ; Xiu-Mei Zhu ; Chang-Qing Gu ; Jing Yin ; Wei Wei ; Wei Yao ; Chao Liu ; Jian-Feng Li ; Guo-Rong Ou ; Min-Nian Wang ; Tong-Yu Fang ; Gui-Jie Wang ; Yao-Hui Qiu ; Huai-Huan Wu ; Fu-Huan Chao ; Jun-Wen LiSource :
- Journal of virological methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- Chine, Culture virale, Désinfection (), Eaux d'égout (virologie), Filtration (), Fèces (virologie), Humains, Hôpitaux, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (transmission), Syndrome respiratoire aigu sévère (virologie), Virologie (), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Eaux d'égout, Fèces, Syndrome respiratoire aigu sévère.
- Chine, Culture virale, Désinfection, Filtration, Humains, Hôpitaux, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Virologie, Virus du SRAS.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- China, Disinfection (methods), Feces (virology), Filtration (methods), Hospitals, Humans, Polymerase Chain Reaction, SARS Virus (classification), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Sewage (virology), Virology (methods), Virus Cultivation.
- MESH :
- chemical , virology : Sewage.
- geographic : China.
- classification : SARS Virus.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Disinfection, Filtration, Virology.
- transmission : Severe Acute Respiratory Syndrome.
- virology : Feces, Severe Acute Respiratory Syndrome.
- Hospitals, Humans, Polymerase Chain Reaction, Sensitivity and Specificity, Virus Cultivation.
Abstract
The transmission of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is associated with close contact to SARS patients and droplet secretions of those patients. The finding of positive RT-PCR results from stools of SARS patients suggests that stools of SARS patients or sewage containing stools of patients could transmit SARS-CoV. We used a novel style of electropositive filter media particle to concentrate the SARS-CoV from the sewage of two hospitals receiving SARS patients in Beijing. We also used cell culture, RT-PCR and gene sequencing to detect and identify the viruses from sewage. No infectious SARS-CoV contamination was found in any of the samples collected, but the nucleic acid of SARS-CoV could be detected in the sewage from the two hospitals before disinfection. While the RNA was only detected in three samples from the 309th Hospital, the others were negative after disinfection. These findings provide strong evidence that SARS-CoV can be excreted through the stool/urine of patients into sewage system, thus making the sewage system a possible route of transmission.
DOI: 10.1016/j.jviromet.2005.03.022
PubMed: 15964082
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pubmed:15964082Le document en format XML
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<term>Disinfection (methods)</term>
<term>Feces (virology)</term>
<term>Filtration (methods)</term>
<term>Hospitals</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>SARS Virus (classification)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
<term>Severe Acute Respiratory Syndrome (transmission)</term>
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<term>Virology (methods)</term>
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<term>Culture virale</term>
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<term>Filtration ()</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Hôpitaux</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Syndrome respiratoire aigu sévère (transmission)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virologie ()</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
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<keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Disinfection</term>
<term>Filtration</term>
<term>Virology</term>
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</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Eaux d'égout</term>
<term>Fèces</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Hospitals</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
<term>Virus Cultivation</term>
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<term>Culture virale</term>
<term>Désinfection</term>
<term>Filtration</term>
<term>Humains</term>
<term>Hôpitaux</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Virologie</term>
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<front><div type="abstract" xml:lang="en">The transmission of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is associated with close contact to SARS patients and droplet secretions of those patients. The finding of positive RT-PCR results from stools of SARS patients suggests that stools of SARS patients or sewage containing stools of patients could transmit SARS-CoV. We used a novel style of electropositive filter media particle to concentrate the SARS-CoV from the sewage of two hospitals receiving SARS patients in Beijing. We also used cell culture, RT-PCR and gene sequencing to detect and identify the viruses from sewage. No infectious SARS-CoV contamination was found in any of the samples collected, but the nucleic acid of SARS-CoV could be detected in the sewage from the two hospitals before disinfection. While the RNA was only detected in three samples from the 309th Hospital, the others were negative after disinfection. These findings provide strong evidence that SARS-CoV can be excreted through the stool/urine of patients into sewage system, thus making the sewage system a possible route of transmission.</div>
</front>
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