[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].
Identifieur interne : 000F08 ( Ncbi/Curation ); précédent : 000F07; suivant : 000F09[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].
Auteurs : Rong Ye [République populaire de Chine] ; Jin-Cao Pan ; Zhi-Cheng Huang ; Heng Wang ; Hao-Qiu Wang ; Dong-Fang Wei ; Ke Xu ; Hong-Gen Wen ; Kang-Kai ChenSource :
- Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [ 0253-9624 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (génétique), ARN viral (métabolisme), Adolescent, Adulte, Adulte d'âge moyen, Humains, Jeune adulte, RT-PCR (), Reproductibilité des résultats, Sensibilité et spécificité, Sujet âgé, Sujet âgé de 80 ans ou plus, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Séquence nucléotidique, Virus du SRAS (génétique).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ARN viral, Virus du SRAS.
- métabolisme : ARN viral.
- virologie : Syndrome respiratoire aigu sévère.
- Adolescent, Adulte, Adulte d'âge moyen, Humains, Jeune adulte, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Sujet âgé, Sujet âgé de 80 ans ou plus, Séquence nucléotidique.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Base Sequence, Humans, Middle Aged, RNA, Viral (genetics), RNA, Viral (metabolism), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (genetics), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Young Adult.
- MESH :
- chemical , genetics : RNA, Viral.
- chemical , metabolism : RNA, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Aged, Aged, 80 and over, Base Sequence, Humans, Middle Aged, Reproducibility of Results, Sensitivity and Specificity, Young Adult.
Abstract
To detect the RNA of severe acute respiratory syndrome virus (SARS-CoV) by using reverse transcription polymerase chain reaction (RT-PCR) targeted for a two loci and a modified nested real-time RT-PCR as to improving the reliability and sensitivity of tests.
PubMed: 15842838
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pubmed:15842838Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology Test, Hangzhou Center for Disease Control and Prevention, Hangzhou 310006, China.</nlm:affiliation>
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<author><name sortKey="Pan, Jin Cao" sort="Pan, Jin Cao" uniqKey="Pan J" first="Jin-Cao" last="Pan">Jin-Cao Pan</name>
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<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Base Sequence</term>
<term>Humans</term>
<term>Middle Aged</term>
<term>RNA, Viral (genetics)</term>
<term>RNA, Viral (metabolism)</term>
<term>Reproducibility of Results</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>Sensitivity and Specificity</term>
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<term>Adulte</term>
<term>Adulte d'âge moyen</term>
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<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
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<term>Sujet âgé de 80 ans ou plus</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Séquence nucléotidique</term>
<term>Virus du SRAS (génétique)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
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<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Base Sequence</term>
<term>Humans</term>
<term>Middle Aged</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
<term>Young Adult</term>
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<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Humains</term>
<term>Jeune adulte</term>
<term>RT-PCR</term>
<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
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<front><div type="abstract" xml:lang="en">To detect the RNA of severe acute respiratory syndrome virus (SARS-CoV) by using reverse transcription polymerase chain reaction (RT-PCR) targeted for a two loci and a modified nested real-time RT-PCR as to improving the reliability and sensitivity of tests.</div>
</front>
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