[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].
Identifieur interne : 002778 ( PubMed/Corpus ); précédent : 002777; suivant : 002779[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].
Auteurs : Rong Ye ; Jin-Cao Pan ; Zhi-Cheng Huang ; Heng Wang ; Hao-Qiu Wang ; Dong-Fang Wei ; Ke Xu ; Hong-Gen Wen ; Kang-Kai ChenSource :
- Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [ 0253-9624 ] ; 2005.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Base Sequence, Humans, Middle Aged, RNA, Viral (genetics), RNA, Viral (metabolism), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (genetics), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Young Adult.
- MESH :
- chemical , genetics : RNA, Viral.
- chemical , metabolism : RNA, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Aged, Aged, 80 and over, Base Sequence, Humans, Middle Aged, Reproducibility of Results, Sensitivity and Specificity, Young Adult.
Abstract
To detect the RNA of severe acute respiratory syndrome virus (SARS-CoV) by using reverse transcription polymerase chain reaction (RT-PCR) targeted for a two loci and a modified nested real-time RT-PCR as to improving the reliability and sensitivity of tests.
PubMed: 15842838
Links to Exploration step
pubmed:15842838Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].</title><author><name sortKey="Ye, Rong" sort="Ye, Rong" uniqKey="Ye R" first="Rong" last="Ye">Rong Ye</name><affiliation><nlm:affiliation>Department of Microbiology Test, Hangzhou Center for Disease Control and Prevention, Hangzhou 310006, China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Jin Cao" sort="Pan, Jin Cao" uniqKey="Pan J" first="Jin-Cao" last="Pan">Jin-Cao Pan</name></author><author><name sortKey="Huang, Zhi Cheng" sort="Huang, Zhi Cheng" uniqKey="Huang Z" first="Zhi-Cheng" last="Huang">Zhi-Cheng Huang</name></author><author><name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name></author><author><name sortKey="Wang, Hao Qiu" sort="Wang, Hao Qiu" uniqKey="Wang H" first="Hao-Qiu" last="Wang">Hao-Qiu Wang</name></author><author><name sortKey="Wei, Dong Fang" sort="Wei, Dong Fang" uniqKey="Wei D" first="Dong-Fang" last="Wei">Dong-Fang Wei</name></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name></author><author><name sortKey="Wen, Hong Gen" sort="Wen, Hong Gen" uniqKey="Wen H" first="Hong-Gen" last="Wen">Hong-Gen Wen</name></author><author><name sortKey="Chen, Kang Kai" sort="Chen, Kang Kai" uniqKey="Chen K" first="Kang-Kai" last="Chen">Kang-Kai Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15842838</idno><idno type="pmid">15842838</idno><idno type="wicri:Area/PubMed/Corpus">002778</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002778</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].</title><author><name sortKey="Ye, Rong" sort="Ye, Rong" uniqKey="Ye R" first="Rong" last="Ye">Rong Ye</name><affiliation><nlm:affiliation>Department of Microbiology Test, Hangzhou Center for Disease Control and Prevention, Hangzhou 310006, China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Jin Cao" sort="Pan, Jin Cao" uniqKey="Pan J" first="Jin-Cao" last="Pan">Jin-Cao Pan</name></author><author><name sortKey="Huang, Zhi Cheng" sort="Huang, Zhi Cheng" uniqKey="Huang Z" first="Zhi-Cheng" last="Huang">Zhi-Cheng Huang</name></author><author><name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name></author><author><name sortKey="Wang, Hao Qiu" sort="Wang, Hao Qiu" uniqKey="Wang H" first="Hao-Qiu" last="Wang">Hao-Qiu Wang</name></author><author><name sortKey="Wei, Dong Fang" sort="Wei, Dong Fang" uniqKey="Wei D" first="Dong-Fang" last="Wei">Dong-Fang Wei</name></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name></author><author><name sortKey="Wen, Hong Gen" sort="Wen, Hong Gen" uniqKey="Wen H" first="Hong-Gen" last="Wen">Hong-Gen Wen</name></author><author><name sortKey="Chen, Kang Kai" sort="Chen, Kang Kai" uniqKey="Chen K" first="Kang-Kai" last="Chen">Kang-Kai Chen</name></author></analytic><series><title level="j">Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</title><idno type="ISSN">0253-9624</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Base Sequence</term><term>Humans</term><term>Middle Aged</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Reproducibility of Results</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Base Sequence</term><term>Humans</term><term>Middle Aged</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To detect the RNA of severe acute respiratory syndrome virus (SARS-CoV) by using reverse transcription polymerase chain reaction (RT-PCR) targeted for a two loci and a modified nested real-time RT-PCR as to improving the reliability and sensitivity of tests.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15842838</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>03</Day></DateCompleted><DateRevised><Year>2005</Year><Month>04</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0253-9624</ISSN><JournalIssue CitedMedium="Print"><Volume>39</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</Title><ISOAbbreviation>Zhonghua Yu Fang Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction].</ArticleTitle><Pagination><MedlinePgn>129-32</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To detect the RNA of severe acute respiratory syndrome virus (SARS-CoV) by using reverse transcription polymerase chain reaction (RT-PCR) targeted for a two loci and a modified nested real-time RT-PCR as to improving the reliability and sensitivity of tests.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients, 4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced. Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi-nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT-PCR and a modified nested real-time RT-PCR, were employed to detect SARS-CoV RNA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Two positives were found in the 3 SARS probable patients, and none positive in 4 SARS suspect patients and other 27 patients with fever, using the nested RT-PCR. The sequence of the nested RT-PCR product from one SARS probable patient was identified with the counterpart of SARS-CoV genomes published in public database. The results of the hemi-nested RT-PCR, the real-time RT-PCR and the modified nested real-time RT-PCR in the 3 SARS patients were consistent with the one of the nested RT-PCR. During detecting specimen with low copies of RNA, a weak positive signal was produced after about 35 cycles in the real-time RT-PCR, but a strong positive signal was found only after 10 cycles in the modified nested real-time RT-PCR.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">It might improve the reliability of test by employing RT-PCR targeted for two or more fragments in SARS-CoV genome. The modified nested real-time RT-PCR might have higher sensitivity than the routine real-time RT-PCR.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Rong</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Microbiology Test, Hangzhou Center for Disease Control and Prevention, Hangzhou 310006, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Jin-cao</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Zhi-cheng</ForeName><Initials>ZC</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Heng</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hao-qiu</ForeName><Initials>HQ</Initials></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Dong-fang</ForeName><Initials>DF</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Ke</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Hong-gen</ForeName><Initials>HG</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Kang-kai</ForeName><Initials>KK</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Yu Fang Yi Xue Za Zhi</MedlineTA><NlmUniqueID>7904962</NlmUniqueID><ISSNLinking>0253-9624</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>4</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>4</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15842838</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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