SrasV1, Ncbi, Curation, bibRecord, 000D35

Development and Characterization of a Severe Acute Respiratory Syndrome—Associated Coronavirus—Neutralizing Human Monoclonal Antibody That Provides Effective Immunoprophylaxis in Mice

Identifieur interne : 000D35 ( Ncbi/Curation ); précédent : 000D34; suivant : 000D36

Development and Characterization of a Severe Acute Respiratory Syndrome—Associated Coronavirus—Neutralizing Human Monoclonal Antibody That Provides Effective Immunoprophylaxis in Mice

Auteurs : Thomas C. Greenough ; Gregory J. Babcock ; Anjeanette Roberts [États-Unis] ; Hector J. Hernandez ; William D. Thomas ; Jennifer A. Coccia ; Robert F. Graziano [États-Unis] ; Mohan Srinivasan [États-Unis] ; Israel Lowy [États-Unis] ; Robert W. Finberg ; Kanta Subbarao [États-Unis] ; Leatrice Vogel [États-Unis] ; Mohan Somasundaran ; Katherine Luzuriaga ; John L. Sullivan ; Donna M. Ambrosino

Source :

The Journal of Infectious Diseases [ 0022-1899 ] ; 2005.

RBID : PMC:7110081

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (therapeutic use), Antibodies, Viral (therapeutic use), Cells, Cultured, Disease Models, Animal, Epitope Mapping, Epitopes (immunology), Female, Immunization, Passive, Lung (virology), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Mice, Transgenic, Neutralization Tests, Protein Binding, SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology).
MESH :
- chemical , immunology : Epitopes, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , therapeutic use : Antibodies, Monoclonal, Antibodies, Viral.
- immunology : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Lung.
- Animals, Cells, Cultured, Disease Models, Animal, Epitope Mapping, Female, Immunization, Passive, Mice, Mice, Inbred BALB C, Mice, Transgenic, Neutralization Tests, Protein Binding, Spike Glycoprotein, Coronavirus.

Abstract

Background. Severe acute respiratory syndrome (SARS) remains a significant public health concern after the epidemic in 2003. Human monoclonal antibodies (MAbs) that neutralize SARS-associated coronavirus (SARSCoV) could provide protection for exposed individuals.

Methods. Transgenic mice with human immunoglobulin genes were immunized with the recombinant major surface (S) glycoprotein ectodomain of SARS-CoV. Epitopes of 2 neutralizing MAbs derived from these mice were mapped and evaluated in a murine model of SARS-CoV infection.

Results. Both MAbs bound to S glycoprotein expressed on transfected cells but differed in their ability to block binding of S glycoprotein to Vero E6 cells. Immunoprecipitation analysis revealed 2 antibody-binding epitopes: one MAb (201) bound within the receptor-binding domain at aa 490–510, and the other MAb (68) bound externally to the domain at aa 130–150. Mice that received 40 mg/kg of either MAb prior to challenge with SARS-CoV were completely protected from virus replication in the lungs, and doses as low as 1.6 mg/kg offered significant protection.

Conclusions. Two neutralizing epitopes were defined for MAbs to SARS-CoV S glycoprotein. Antibodies to both epitopes protected mice against SARS-CoV challenge. Clinical trials are planned to test MAb 201, a fully human MAb specific for the epitope within the receptor-binding region.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110081

DOI: 10.1086/427242
PubMed: 15655773
PubMed Central: 7110081

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PMC:7110081

Le document en format XML

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,<addr-line>Bloomsbury, New Jersey</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">New Jersey</region>
</placeName>
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,<addr-line>Worcester</addr-line>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<affiliation wicri:level="2"><nlm:aff id="aff3"><institution>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health</institution>
,<addr-line>Bethesda, Maryland</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Maryland</region>
</placeName>
<wicri:cityArea>Bethesda</wicri:cityArea>
</affiliation>
</author>
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<affiliation wicri:level="2"><nlm:aff id="aff3"><institution>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health</institution>
,<addr-line>Bethesda, Maryland</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Maryland</region>
</placeName>
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,<addr-line>Worcester</addr-line>
</nlm:aff>
</affiliation>
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<author><name sortKey="Luzuriaga, Katherine" sort="Luzuriaga, Katherine" uniqKey="Luzuriaga K" first="Katherine" last="Luzuriaga">Katherine Luzuriaga</name>
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,<addr-line>Worcester</addr-line>
</nlm:aff>
</affiliation>
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,<addr-line>Worcester</addr-line>
</nlm:aff>
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<author><name sortKey="Ambrosino, Donna M" sort="Ambrosino, Donna M" uniqKey="Ambrosino D" first="Donna M." last="Ambrosino">Donna M. Ambrosino</name>
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,<addr-line>Jamaica Plain</addr-line>
</nlm:aff>
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<term>Antibodies, Monoclonal (therapeutic use)</term>
<term>Antibodies, Viral (therapeutic use)</term>
<term>Cells, Cultured</term>
<term>Disease Models, Animal</term>
<term>Epitope Mapping</term>
<term>Epitopes (immunology)</term>
<term>Female</term>
<term>Immunization, Passive</term>
<term>Lung (virology)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Mice, Transgenic</term>
<term>Neutralization Tests</term>
<term>Protein Binding</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (usage thérapeutique)</term>
<term>Anticorps monoclonaux (usage thérapeutique)</term>
<term>Cartographie épitopique</term>
<term>Cellules cultivées</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Immunisation passive</term>
<term>Liaison aux protéines</term>
<term>Modèles animaux de maladie humaine</term>
<term>Poumon (virologie)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Souris transgéniques</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Tests de neutralisation</term>
<term>Virus du SRAS (immunologie)</term>
<term>Épitopes (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
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<term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
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<term>Anticorps monoclonaux</term>
</keywords>
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</keywords>
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</keywords>
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<term>Cells, Cultured</term>
<term>Disease Models, Animal</term>
<term>Epitope Mapping</term>
<term>Female</term>
<term>Immunization, Passive</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Mice, Transgenic</term>
<term>Neutralization Tests</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cartographie épitopique</term>
<term>Cellules cultivées</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Immunisation passive</term>
<term>Liaison aux protéines</term>
<term>Modèles animaux de maladie humaine</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Souris transgéniques</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Tests de neutralisation</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p><bold><italic>Background.</italic>
</bold>
 Severe acute respiratory syndrome (SARS) remains a significant public health concern after the epidemic in 2003. Human monoclonal antibodies (MAbs) that neutralize SARS-associated coronavirus (SARSCoV) could provide protection for exposed individuals.</p>
<p><bold><italic>Methods.</italic>
</bold>
 Transgenic mice with human immunoglobulin genes were immunized with the recombinant major surface (S) glycoprotein ectodomain of SARS-CoV. Epitopes of 2 neutralizing MAbs derived from these mice were mapped and evaluated in a murine model of SARS-CoV infection.</p>
<p><bold><italic>Results.</italic>
</bold>
 Both MAbs bound to S glycoprotein expressed on transfected cells but differed in their ability to block binding of S glycoprotein to Vero E6 cells. Immunoprecipitation analysis revealed 2 antibody-binding epitopes: one MAb (201) bound within the receptor-binding domain at aa 490–510, and the other MAb (68) bound externally to the domain at aa 130–150. Mice that received 40 mg/kg of either MAb prior to challenge with SARS-CoV were completely protected from virus replication in the lungs, and doses as low as 1.6 mg/kg offered significant protection.</p>
<p><bold><italic>Conclusions.</italic>
</bold>
 Two neutralizing epitopes were defined for MAbs to SARS-CoV S glycoprotein. Antibodies to both epitopes protected mice against SARS-CoV challenge. Clinical trials are planned to test MAb 201, a fully human MAb specific for the epitope within the receptor-binding region.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name>
</author>
<author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name>
</author>
<author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Bisht, H" uniqKey="Bisht H">H Bisht</name>
</author>
<author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name>
</author>
<author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bukreyev, A" uniqKey="Bukreyev A">A Bukreyev</name>
</author>
<author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name>
</author>
<author><name sortKey="Buchholz, Uj" uniqKey="Buchholz U">UJ Buchholz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name>
</author>
<author><name sortKey="Kong, Wp" uniqKey="Kong W">WP Kong</name>
</author>
<author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sawyer, La" uniqKey="Sawyer L">LA Sawyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Popova, R" uniqKey="Popova R">R Popova</name>
</author>
<author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bonavia, A" uniqKey="Bonavia A">A Bonavia</name>
</author>
<author><name sortKey="Zelus, Bd" uniqKey="Zelus B">BD Zelus</name>
</author>
<author><name sortKey="Wentworth, De" uniqKey="Wentworth D">DE Wentworth</name>
</author>
<author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ Talbot</name>
</author>
<author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuo, L" uniqKey="Kuo L">L Kuo</name>
</author>
<author><name sortKey="Godeke, Gj" uniqKey="Godeke G">GJ Godeke</name>
</author>
<author><name sortKey="Raamsman, Mj" uniqKey="Raamsman M">MJ Raamsman</name>
</author>
<author><name sortKey="Masters, Ps" uniqKey="Masters P">PS Masters</name>
</author>
<author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gallagher, Tm" uniqKey="Gallagher T">TM Gallagher</name>
</author>
<author><name sortKey="Buchmeier, Mj" uniqKey="Buchmeier M">MJ Buchmeier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
<author><name sortKey="Moore, Mj" uniqKey="Moore M">MJ Moore</name>
</author>
<author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N Vasilieva</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Babcock, Gj" uniqKey="Babcock G">GJ Babcock</name>
</author>
<author><name sortKey="Esshaki, Dj" uniqKey="Esshaki D">DJ Esshaki</name>
</author>
<author><name sortKey="Thomas, Wd" uniqKey="Thomas W">WD Thomas</name>
</author>
<author><name sortKey="Ambrosino, Dm" uniqKey="Ambrosino D">DM Ambrosino</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wong, Sk" uniqKey="Wong S">SK Wong</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
<author><name sortKey="Moore, Mj" uniqKey="Moore M">MJ Moore</name>
</author>
<author><name sortKey="Choe, H" uniqKey="Choe H">H Choe</name>
</author>
<author><name sortKey="Farzan, M" uniqKey="Farzan M">M Farzan</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Witte, Kh" uniqKey="Witte K">KH Witte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reed, Lv" uniqKey="Reed L">LV Reed</name>
</author>
<author><name sortKey="Muench, H" uniqKey="Muench H">H Muench</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name>
</author>
<author><name sortKey="Mcauliffe, J" uniqKey="Mcauliffe J">J McAuliffe</name>
</author>
<author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rich, Rl" uniqKey="Rich R">RL Rich</name>
</author>
<author><name sortKey="Myszka, Dg" uniqKey="Myszka D">DG Myszka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Johnson, S" uniqKey="Johnson S">S Johnson</name>
</author>
<author><name sortKey="Griego, Sd" uniqKey="Griego S">SD Griego</name>
</author>
<author><name sortKey="Pfarr, Ds" uniqKey="Pfarr D">DS Pfarr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meissner, Hc" uniqKey="Meissner H">HC Meissner</name>
</author>
<author><name sortKey="Long, Ss" uniqKey="Long S">SS Long</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J Sui</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
<author><name sortKey="Murakami, A" uniqKey="Murakami A">A Murakami</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yeh, Sh" uniqKey="Yeh S">SH Yeh</name>
</author>
<author><name sortKey="Wang, Hy" uniqKey="Wang H">HY Wang</name>
</author>
<author><name sortKey="Tsai, Cy" uniqKey="Tsai C">CY Tsai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Zheng, B" uniqKey="Zheng B">B Zheng</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>

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Development and Characterization of a Severe Acute Respiratory Syndrome—Associated Coronavirus—Neutralizing Human Monoclonal Antibody That Provides Effective Immunoprophylaxis in Mice

Development and Characterization of a Severe Acute Respiratory Syndrome—Associated Coronavirus—Neutralizing Human Monoclonal Antibody That Provides Effective Immunoprophylaxis in Mice

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