Susceptibility of different eukaryotic cell lines to SARS-coronavirus.
Identifieur interne : 000D21 ( Ncbi/Curation ); précédent : 000D20; suivant : 000D22Susceptibility of different eukaryotic cell lines to SARS-coronavirus.
Auteurs : K. Hattermann [Allemagne] ; M A Müller ; A. Nitsche ; S. Wendt ; O. Donoso Mantke ; M. NiedrigSource :
- Archives of virology [ 0304-8608 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (métabolisme), Cellules Vero, Cellules eucaryotes (virologie), Chats, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, RT-PCR, Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Souris, Suidae, Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
- MESH :
- génétique : Carboxypeptidases, Récepteurs viraux, Virus du SRAS.
- métabolisme : Carboxypeptidases, Récepteurs viraux.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Cellules eucaryotes.
- Animaux, Cellules Vero, Chats, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, RT-PCR, Souris, Suidae.
English descriptors
- KwdEn :
- Animals, Carboxypeptidases (genetics), Carboxypeptidases (metabolism), Cats, Cell Line, Chlorocebus aethiops, Eukaryotic Cells (virology), Humans, Mice, Peptidyl-Dipeptidase A, Receptors, Virus (genetics), Receptors, Virus (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Swine, Vero Cells.
- MESH :
- chemical , genetics : Carboxypeptidases, Receptors, Virus.
- chemical , metabolism : Carboxypeptidases, Receptors, Virus.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- virology : Eukaryotic Cells.
- Animals, Cats, Cell Line, Chlorocebus aethiops, Humans, Mice, Peptidyl-Dipeptidase A, Reverse Transcriptase Polymerase Chain Reaction, Swine, Vero Cells.
Abstract
In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.
DOI: 10.1007/s00705-004-0461-1
PubMed: 15645376
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