Susceptibility of different eukaryotic cell lines to SARS-coronavirus.
Identifieur interne : 002451 ( PubMed/Checkpoint ); précédent : 002450; suivant : 002452Susceptibility of different eukaryotic cell lines to SARS-coronavirus.
Auteurs : K. Hattermann [Allemagne] ; M A Müller ; A. Nitsche ; S. Wendt ; O. Donoso Mantke ; M. NiedrigSource :
- Archives of virology [ 0304-8608 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (métabolisme), Cellules Vero, Cellules eucaryotes (virologie), Chats, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, RT-PCR, Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Souris, Suidae, Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
- MESH :
- génétique : Carboxypeptidases, Récepteurs viraux, Virus du SRAS.
- métabolisme : Carboxypeptidases, Récepteurs viraux.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Cellules eucaryotes.
- Animaux, Cellules Vero, Chats, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, RT-PCR, Souris, Suidae.
English descriptors
- KwdEn :
- Animals, Carboxypeptidases (genetics), Carboxypeptidases (metabolism), Cats, Cell Line, Chlorocebus aethiops, Eukaryotic Cells (virology), Humans, Mice, Peptidyl-Dipeptidase A, Receptors, Virus (genetics), Receptors, Virus (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Swine, Vero Cells.
- MESH :
- chemical , genetics : Carboxypeptidases, Receptors, Virus.
- chemical , metabolism : Carboxypeptidases, Receptors, Virus.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- virology : Eukaryotic Cells.
- Animals, Cats, Cell Line, Chlorocebus aethiops, Humans, Mice, Peptidyl-Dipeptidase A, Reverse Transcriptase Polymerase Chain Reaction, Swine, Vero Cells.
Abstract
In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.
DOI: 10.1007/s00705-004-0461-1
PubMed: 15645376
Affiliations:
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Virol.</ISOAbbreviation></Journal><ArticleTitle>Susceptibility of different eukaryotic cell lines to SARS-coronavirus.</ArticleTitle><Pagination><MedlinePgn>1023-31</MedlinePgn></Pagination><Abstract><AbstractText>In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hattermann</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Robert Koch-Institut, Center for Biological Safety 1, Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>M A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Nitsche</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Wendt</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Donoso Mantke</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Niedrig</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType 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IdType="pmc">PMC7086824</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><noCountry><name sortKey="Donoso Mantke, O" sort="Donoso Mantke, O" uniqKey="Donoso Mantke O" first="O" last="Donoso Mantke">O. Donoso Mantke</name><name sortKey="Muller, M A" sort="Muller, M A" uniqKey="Muller M" first="M A" last="Müller">M A Müller</name><name sortKey="Niedrig, M" sort="Niedrig, M" uniqKey="Niedrig M" first="M" last="Niedrig">M. Niedrig</name><name sortKey="Nitsche, A" sort="Nitsche, A" uniqKey="Nitsche A" first="A" last="Nitsche">A. Nitsche</name><name sortKey="Wendt, S" sort="Wendt, S" uniqKey="Wendt S" first="S" last="Wendt">S. Wendt</name></noCountry><country name="Allemagne"><region name="Berlin"><name sortKey="Hattermann, K" sort="Hattermann, K" uniqKey="Hattermann K" first="K" last="Hattermann">K. Hattermann</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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