Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with Pneumonia
Identifieur interne : 000C70 ( Ncbi/Curation ); précédent : 000C69; suivant : 000C71Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with Pneumonia
Auteurs : Patrick C. Y. Woo ; Susanna K. P. Lau ; Chung-Ming Chu ; Kwok-Hung Chan ; Hoi-Wah Tsoi ; Yi Huang ; Beatrice H. L. Wong ; Rosana W. S. Poon ; James J. Cai ; Wei-Kwang Luk ; Leo L. M. Poon ; Samson S. Y. Wong ; Yi Guan ; J. S. Malik Peiris ; Kwok-Yung YuenSource :
- Journal of Virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Cadres ouverts de lecture, Coronavirus (), Coronavirus (génétique), Données de séquences moléculaires, Gènes pol, Génome viral, Humains, Mâle, Partie nasale du pharynx (virologie), Phylogénie, Pneumopathie virale (virologie), RT-PCR, Sujet âgé, Séquence d'acides aminés, Séquence nucléotidique, Technique de Western, Test ELISA.
- MESH :
- génétique : Coronavirus.
- virologie : Partie nasale du pharynx, Pneumopathie virale.
- Analyse de séquence d'ADN, Cadres ouverts de lecture, Coronavirus, Données de séquences moléculaires, Gènes pol, Génome viral, Humains, Mâle, Phylogénie, RT-PCR, Sujet âgé, Séquence d'acides aminés, Séquence nucléotidique, Technique de Western, Test ELISA.
English descriptors
- KwdEn :
- Aged, Amino Acid Sequence, Base Sequence, Blotting, Western, Coronavirus (classification), Coronavirus (genetics), Enzyme-Linked Immunosorbent Assay, Genes, pol, Genome, Viral, Humans, Male, Molecular Sequence Data, Nasopharynx (virology), Open Reading Frames, Phylogeny, Pneumonia, Viral (virology), Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
- MESH :
- classification : Coronavirus.
- genetics : Coronavirus.
- virology : Nasopharynx, Pneumonia, Viral.
- Aged, Amino Acid Sequence, Base Sequence, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Genes, pol, Genome, Viral, Humans, Male, Molecular Sequence Data, Open Reading Frames, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Despite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 106 copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 106 copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.
Url:
DOI: 10.1128/JVI.79.2.884-895.2005
PubMed: 15613317
PubMed Central: 538593
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PMC:538593Le document en format XML
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Y." last="Woo">Patrick C. Y. Woo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chu, Chung Ming" sort="Chu, Chung Ming" uniqKey="Chu C" first="Chung-Ming" last="Chu">Chung-Ming Chu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. Poon</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Cai, James J" sort="Cai, James J" uniqKey="Cai J" first="James J." last="Cai">James J. Cai</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Luk, Wei Kwang" sort="Luk, Wei Kwang" uniqKey="Luk W" first="Wei-Kwang" last="Luk">Wei-Kwang Luk</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wong, Samson S Y" sort="Wong, Samson S Y" uniqKey="Wong S" first="Samson S. Y." last="Wong">Samson S. Y. Wong</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Blotting, Western</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Genes, pol</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Molecular Sequence Data</term><term>Nasopharynx (virology)</term><term>Open Reading Frames</term><term>Phylogeny</term><term>Pneumonia, Viral (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cadres ouverts de lecture</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Données de séquences moléculaires</term><term>Gènes pol</term><term>Génome viral</term><term>Humains</term><term>Mâle</term><term>Partie nasale du pharynx (virologie)</term><term>Phylogénie</term><term>Pneumopathie virale (virologie)</term><term>RT-PCR</term><term>Sujet âgé</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Western</term><term>Test ELISA</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Partie nasale du pharynx</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Nasopharynx</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Genes, pol</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>Phylogeny</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cadres ouverts de lecture</term><term>Coronavirus</term><term>Données de séquences moléculaires</term><term>Gènes pol</term><term>Génome viral</term><term>Humains</term><term>Mâle</term><term>Phylogénie</term><term>RT-PCR</term><term>Sujet âgé</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Western</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Despite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 10<sup>6</sup> copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 10<sup>6</sup> copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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