False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide.
Identifieur interne : 000C51 ( Ncbi/Curation ); précédent : 000C50; suivant : 000C52False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide.
Auteurs : Patrick C Y. Woo [Hong Kong] ; Susanna K P. Lau ; Beatrice H L. Wong ; Kwok-Hung Chan ; Wai-Ting Hui ; Grace S W. Kwan ; J S Malik Peiris ; Robert B. Couch ; Kwok-Yung YuenSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Faux positifs, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Peptides (génétique), Peptides (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Réactions croisées, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Test ELISA, Virus du SRAS (immunologie).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines nucléocapside.
- immunologie : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines recombinantes, Virus du SRAS.
- sang : Anticorps antiviraux.
- virologie : Syndrome respiratoire aigu sévère.
- Faux positifs, Glycoprotéine de spicule des coronavirus, Humains, Réactions croisées, Technique de Western, Test ELISA.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Blotting, Western, Cross Reactions, Enzyme-Linked Immunosorbent Assay, False Positive Reactions, Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Peptides (genetics), Peptides (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Membrane Glycoproteins, Nucleocapsid Proteins, Peptides, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Peptides, Recombinant Proteins, Viral Envelope Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Blotting, Western, Cross Reactions, Enzyme-Linked Immunosorbent Assay, False Positive Reactions, Humans, Spike Glycoprotein, Coronavirus.
Abstract
Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.
DOI: 10.1128/JCM.42.12.5885-5888.2004
PubMed: 15583332
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pubmed:15583332Le document en format XML
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Woo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong. hkumicro@hkucc.hku.hk.</nlm:affiliation><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea><wicri:noRegion>Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H L" last="Wong">Beatrice H L. 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Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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