Serveur d'exploration SRAS

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False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide.

Identifieur interne : 002A13 ( PubMed/Curation ); précédent : 002A12; suivant : 002A14

False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide.

Auteurs : Patrick C Y. Woo [Hong Kong] ; Susanna K P. Lau ; Beatrice H L. Wong ; Kwok-Hung Chan ; Wai-Ting Hui ; Grace S W. Kwan ; J S Malik Peiris ; Robert B. Couch ; Kwok-Yung Yuen

Source :

RBID : pubmed:15583332

Descripteurs français

English descriptors

Abstract

Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.

DOI: 10.1128/JCM.42.12.5885-5888.2004
PubMed: 15583332

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pubmed:15583332

Le document en format XML

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