Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.
Identifieur interne : 000B19 ( Ncbi/Curation ); précédent : 000B18; suivant : 000B20Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.
Auteurs : Paul K S. Chan [République populaire de Chine] ; W K To ; Esther Y M. Liu ; T K Ng ; John S. Tam ; Joseph J Y. Sung ; Jean-Michel Lacroix ; Michel HoudeSource :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Coronavirus (génétique), Coronavirus (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Immunoglobuline G (analyse), Immunoglobuline G (immunologie), Mâle, Nourrisson, Nucléocapside (analyse), Sensibilité et spécificité, Sujet âgé, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Test ELISA, Tests sérologiques (), Virus du SRAS (immunologie), Études d'évaluation comme sujet.
- MESH :
- analyse : Immunoglobuline G, Nucléocapside.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Coronavirus.
- immunologie : Immunoglobuline G, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Coronavirus.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Adolescent, Adulte, Adulte d'âge moyen, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Mâle, Nourrisson, Sensibilité et spécificité, Sujet âgé, Test ELISA, Tests sérologiques, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Child, Child, Preschool, Coronavirus (genetics), Coronavirus (isolation & purification), Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, Female, Humans, Immunoglobulin G (analysis), Immunoglobulin G (immunology), Infant, Male, Middle Aged, Nucleocapsid (analysis), SARS Virus (immunology), Sensitivity and Specificity, Serologic Tests (methods), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , analysis : Immunoglobulin G.
- analysis : Nucleocapsid.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : Coronavirus.
- chemical , immunology : Immunoglobulin G, SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : Coronavirus.
- methods : Serologic Tests.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Aged, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, Female, Humans, Infant, Male, Middle Aged, Sensitivity and Specificity.
Abstract
High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.
DOI: 10.1002/jmv.20207
PubMed: 15484283
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pubmed:15484283Le document en format XML
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Chan</name><affiliation wicri:level="4"><nlm:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="To, W K" sort="To, W K" uniqKey="To W" first="W K" last="To">W K To</name></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name></author><author><name sortKey="Ng, T K" sort="Ng, T K" uniqKey="Ng T" first="T K" last="Ng">T K Ng</name></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author><author><name sortKey="Lacroix, Jean Michel" sort="Lacroix, Jean Michel" uniqKey="Lacroix J" first="Jean-Michel" last="Lacroix">Jean-Michel Lacroix</name></author><author><name sortKey="Houde, Michel" sort="Houde, Michel" uniqKey="Houde M" first="Michel" last="Houde">Michel Houde</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15484283</idno><idno type="pmid">15484283</idno><idno type="doi">10.1002/jmv.20207</idno><idno type="wicri:Area/PubMed/Corpus">002B14</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B14</idno><idno type="wicri:Area/PubMed/Curation">002B14</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B14</idno><idno type="wicri:Area/PubMed/Checkpoint">002D18</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D18</idno><idno type="wicri:Area/Ncbi/Merge">000B19</idno><idno type="wicri:Area/Ncbi/Curation">000B19</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.</title><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. 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Sung</name></author><author><name sortKey="Lacroix, Jean Michel" sort="Lacroix, Jean Michel" uniqKey="Lacroix J" first="Jean-Michel" last="Lacroix">Jean-Michel Lacroix</name></author><author><name sortKey="Houde, Michel" sort="Houde, Michel" uniqKey="Houde M" first="Michel" last="Houde">Michel Houde</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="ISSN">0146-6615</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Child</term><term>Child, Preschool</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Evaluation Studies as Topic</term><term>Female</term><term>Humans</term><term>Immunoglobulin G (analysis)</term><term>Immunoglobulin G (immunology)</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Nucleocapsid (analysis)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Serologic Tests (methods)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Immunoglobuline G (analyse)</term><term>Immunoglobuline G (immunologie)</term><term>Mâle</term><term>Nourrisson</term><term>Nucléocapside (analyse)</term><term>Sensibilité et spécificité</term><term>Sujet 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qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Child</term><term>Child, Preschool</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Evaluation Studies as Topic</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Nourrisson</term><term>Sensibilité et spécificité</term><term>Sujet âgé</term><term>Test ELISA</term><term>Tests sérologiques</term><term>Études d'évaluation comme sujet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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