Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.
Identifieur interne : 002B14 ( PubMed/Corpus ); précédent : 002B13; suivant : 002B15Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.
Auteurs : Paul K S. Chan ; W K To ; Esther Y M. Liu ; T K Ng ; John S. Tam ; Joseph J Y. Sung ; Jean-Michel Lacroix ; Michel HoudeSource :
- Journal of medical virology [ 0146-6615 ] ; 2004.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Child, Child, Preschool, Coronavirus (genetics), Coronavirus (isolation & purification), Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, Female, Humans, Immunoglobulin G (analysis), Immunoglobulin G (immunology), Infant, Male, Middle Aged, Nucleocapsid (analysis), SARS Virus (immunology), Sensitivity and Specificity, Serologic Tests (methods), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , analysis : Immunoglobulin G.
- analysis : Nucleocapsid.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : Coronavirus.
- chemical , immunology : Immunoglobulin G, SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : Coronavirus.
- methods : Serologic Tests.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Aged, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, Female, Humans, Infant, Male, Middle Aged, Sensitivity and Specificity.
Abstract
High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.
DOI: 10.1002/jmv.20207
PubMed: 15484283
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pubmed:15484283Le document en format XML
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Tam</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author><author><name sortKey="Lacroix, Jean Michel" sort="Lacroix, Jean Michel" uniqKey="Lacroix J" first="Jean-Michel" last="Lacroix">Jean-Michel Lacroix</name></author><author><name sortKey="Houde, Michel" sort="Houde, Michel" uniqKey="Houde M" first="Michel" last="Houde">Michel Houde</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15484283</idno><idno type="pmid">15484283</idno><idno type="doi">10.1002/jmv.20207</idno><idno type="wicri:Area/PubMed/Corpus">002B14</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.</title><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name><affiliation><nlm:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk</nlm:affiliation></affiliation></author><author><name sortKey="To, W K" sort="To, W K" uniqKey="To W" first="W K" last="To">W K To</name></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name></author><author><name sortKey="Ng, T K" sort="Ng, T K" uniqKey="Ng T" first="T K" last="Ng">T K Ng</name></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author><author><name sortKey="Lacroix, Jean Michel" sort="Lacroix, Jean Michel" uniqKey="Lacroix J" first="Jean-Michel" last="Lacroix">Jean-Michel Lacroix</name></author><author><name sortKey="Houde, Michel" sort="Houde, Michel" uniqKey="Houde M" first="Michel" last="Houde">Michel Houde</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="ISSN">0146-6615</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Child</term><term>Child, Preschool</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Evaluation Studies as Topic</term><term>Female</term><term>Humans</term><term>Immunoglobulin G (analysis)</term><term>Immunoglobulin G (immunology)</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Nucleocapsid (analysis)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Serologic Tests (methods)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Nucleocapsid</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Immunoglobulin G</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Serologic Tests</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Child</term><term>Child, Preschool</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Evaluation Studies as Topic</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15484283</PMID><DateCompleted><Year>2005</Year><Month>01</Month><Day>10</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0146-6615</ISSN><JournalIssue CitedMedium="Print"><Volume>74</Volume><Issue>4</Issue><PubDate><Year>2004</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Journal of medical virology</Title><ISOAbbreviation>J. Med. Virol.</ISOAbbreviation></Journal><ArticleTitle>Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody.</ArticleTitle><Pagination><MedlinePgn>517-20</MedlinePgn></Pagination><Abstract><AbstractText>High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.</AbstractText><CopyrightInformation>2004 Wiley-Liss, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Paul K S</ForeName><Initials>PK</Initials><AffiliationInfo><Affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>To</LastName><ForeName>W K</ForeName><Initials>WK</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Esther Y M</ForeName><Initials>EY</Initials></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>T K</ForeName><Initials>TK</Initials></Author><Author ValidYN="Y"><LastName>Tam</LastName><ForeName>John S</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Sung</LastName><ForeName>Joseph J Y</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Lacroix</LastName><ForeName>Jean-Michel</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Houde</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Med Virol</MedlineTA><NlmUniqueID>7705876</NlmUniqueID><ISSNLinking>0146-6615</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin 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