Assembly of human severe acute respiratory syndrome coronavirus-like particles.
Identifieur interne : 000815 ( Ncbi/Curation ); précédent : 000814; suivant : 000816Assembly of human severe acute respiratory syndrome coronavirus-like particles.
Auteurs : Yu Ho [République populaire de Chine] ; Pi-Hsiu Lin ; Catherine Y Y. Liu ; Su-Ping Lee ; Yu-Chan ChaoSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Baculoviridae (métabolisme), Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (ultrastructure), Humains, Lignée cellulaire, Microscopie électronique, Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (ultrastructure), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Protéines recombinantes (ultrastructure), Spodoptera (cytologie), Virus du SRAS (), Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- biosynthèse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes.
- cytologie : Spodoptera.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes.
- métabolisme : Baculoviridae, Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Microscopie électronique, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Baculoviridae (metabolism), Cell Line, Gene Expression, Humans, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (ultrastructure), Microscopy, Electron, Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (ultrastructure), SARS Virus (chemistry), SARS Virus (metabolism), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Spodoptera (cytology), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Envelope Proteins (ultrastructure).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemistry : SARS Virus.
- cytology : Spodoptera.
- metabolism : Baculoviridae, SARS Virus.
- physiology : SARS Virus.
- chemical , ultrastructure : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- Animals, Cell Line, Gene Expression, Humans, Microscopy, Electron, Spike Glycoprotein, Coronavirus.
Abstract
Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.
DOI: 10.1016/j.bbrc.2004.04.111
PubMed: 15147946
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pubmed:15147946Le document en format XML
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Liu</name></author><author><name sortKey="Lee, Su Ping" sort="Lee, Su Ping" uniqKey="Lee S" first="Su-Ping" last="Lee">Su-Ping Lee</name></author><author><name sortKey="Chao, Yu Chan" sort="Chao, Yu Chan" uniqKey="Chao Y" first="Yu-Chan" last="Chao">Yu-Chan Chao</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Baculoviridae (metabolism)</term><term>Cell Line</term><term>Gene Expression</term><term>Humans</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (ultrastructure)</term><term>Microscopy, Electron</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (ultrastructure)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spodoptera (cytology)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Baculoviridae (métabolisme)</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (ultrastructure)</term><term>Humains</term><term>Lignée cellulaire</term><term>Microscopie électronique</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (ultrastructure)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (ultrastructure)</term><term>Spodoptera (cytologie)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Baculoviridae</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Baculoviridae</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Gene Expression</term><term>Humans</term><term>Microscopy, Electron</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Microscopie électronique</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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