Assembly of human severe acute respiratory syndrome coronavirus-like particles.
Identifieur interne : 002D94 ( PubMed/Corpus ); précédent : 002D93; suivant : 002D95Assembly of human severe acute respiratory syndrome coronavirus-like particles.
Auteurs : Yu Ho ; Pi-Hsiu Lin ; Catherine Y Y. Liu ; Su-Ping Lee ; Yu-Chan ChaoSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
English descriptors
- KwdEn :
- Animals, Baculoviridae (metabolism), Cell Line, Gene Expression, Humans, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (ultrastructure), Microscopy, Electron, Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (ultrastructure), SARS Virus (chemistry), SARS Virus (metabolism), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Spodoptera (cytology), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Envelope Proteins (ultrastructure).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemistry : SARS Virus.
- cytology : Spodoptera.
- metabolism : Baculoviridae, SARS Virus.
- physiology : SARS Virus.
- chemical , ultrastructure : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- Animals, Cell Line, Gene Expression, Humans, Microscopy, Electron, Spike Glycoprotein, Coronavirus.
Abstract
Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.
DOI: 10.1016/j.bbrc.2004.04.111
PubMed: 15147946
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pubmed:15147946Le document en format XML
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<author><name sortKey="Ho, Yu" sort="Ho, Yu" uniqKey="Ho Y" first="Yu" last="Ho">Yu Ho</name>
<affiliation><nlm:affiliation>Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, ROC.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Lin, Pi Hsiu" sort="Lin, Pi Hsiu" uniqKey="Lin P" first="Pi-Hsiu" last="Lin">Pi-Hsiu Lin</name>
</author>
<author><name sortKey="Liu, Catherine Y Y" sort="Liu, Catherine Y Y" uniqKey="Liu C" first="Catherine Y Y" last="Liu">Catherine Y Y. Liu</name>
</author>
<author><name sortKey="Lee, Su Ping" sort="Lee, Su Ping" uniqKey="Lee S" first="Su-Ping" last="Lee">Su-Ping Lee</name>
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<author><name sortKey="Chao, Yu Chan" sort="Chao, Yu Chan" uniqKey="Chao Y" first="Yu-Chan" last="Chao">Yu-Chan Chao</name>
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<author><name sortKey="Ho, Yu" sort="Ho, Yu" uniqKey="Ho Y" first="Yu" last="Ho">Yu Ho</name>
<affiliation><nlm:affiliation>Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, ROC.</nlm:affiliation>
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<author><name sortKey="Lin, Pi Hsiu" sort="Lin, Pi Hsiu" uniqKey="Lin P" first="Pi-Hsiu" last="Lin">Pi-Hsiu Lin</name>
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<author><name sortKey="Liu, Catherine Y Y" sort="Liu, Catherine Y Y" uniqKey="Liu C" first="Catherine Y Y" last="Liu">Catherine Y Y. Liu</name>
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<author><name sortKey="Lee, Su Ping" sort="Lee, Su Ping" uniqKey="Lee S" first="Su-Ping" last="Lee">Su-Ping Lee</name>
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<author><name sortKey="Chao, Yu Chan" sort="Chao, Yu Chan" uniqKey="Chao Y" first="Yu-Chan" last="Chao">Yu-Chan Chao</name>
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<term>Baculoviridae (metabolism)</term>
<term>Cell Line</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Membrane Glycoproteins (biosynthesis)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (ultrastructure)</term>
<term>Microscopy, Electron</term>
<term>Recombinant Proteins (biosynthesis)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (ultrastructure)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (metabolism)</term>
<term>SARS Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Spodoptera (cytology)</term>
<term>Viral Envelope Proteins (biosynthesis)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (ultrastructure)</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
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<term>Recombinant Proteins</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
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<front><div type="abstract" xml:lang="en">Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.</div>
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<Abstract><AbstractText>Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.</AbstractText>
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