SrasV1, Ncbi, Curation, bibRecord, 000808

Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.

Identifieur interne : 000808 ( Ncbi/Curation ); précédent : 000807; suivant : 000809

Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.

Auteurs : Erik Lontok [États-Unis] ; Emily Corse ; Carolyn E. Machamer

Source :

Journal of virology [ 0022-538X ] ; 2004.

RBID : pubmed:15140989

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Motifs, Amino Acid Sequence, Dipeptides, Endoplasmic Reticulum (physiology), Golgi Apparatus (physiology), HeLa Cells, Humans, Infectious bronchitis virus (physiology), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (physiology), Molecular Sequence Data, Protein Transport, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (physiology), Virus Assembly.
MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , physiology : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical : Dipeptides, Spike Glycoprotein, Coronavirus.
- physiology : Endoplasmic Reticulum, Golgi Apparatus, Infectious bronchitis virus.
- Amino Acid Motifs, Amino Acid Sequence, HeLa Cells, Humans, Molecular Sequence Data, Protein Transport, Virus Assembly.

Abstract

Coronavirus budding at the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) requires accumulation of the viral envelope proteins at this point in the secretory pathway. Here we demonstrate that the spike (S) protein from the group 3 coronavirus infectious bronchitis virus (IBV) contains a canonical dilysine endoplasmic reticulum retrieval signal (-KKXX-COOH) in its cytoplasmic tail. This signal can retain a chimeric reporter protein in the ERGIC and when mutated allows transport of the full-length S protein as well as the chimera to the plasma membrane. Interestingly, the IBV S protein also contains a tyrosine-based endocytosis signal in its cytoplasmic tail, suggesting that any S protein that escapes the ERGIC will be rapidly endocytosed when it reaches the plasma membrane. We also identified a novel dibasic motif (-KXHXX-COOH) in the cytoplasmic tails of S proteins from group 1 coronaviruses and from the newly identified coronavirus implicated in severe acute respiratory syndrome. This dibasic motif also retained a reporter protein in the ERGIC, similar to the dilysine motif in IBV S. The cytoplasmic tails of S proteins from group 2 coronaviruses lack an intracellular localization signal. The inherent differences in S-protein trafficking could point to interesting variations in pathogenesis of coronaviruses, since increased levels of surface S protein could promote syncytium formation and direct cell-to-cell spread of the infection.

DOI: 10.1128/JVI.78.11.5913-5922.2004
PubMed: 15140989

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002E00
to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002E00
to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002C22
to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000808

Links to Exploration step

pubmed:15140989

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.</title>
<author><name sortKey="Lontok, Erik" sort="Lontok, Erik" uniqKey="Lontok E" first="Erik" last="Lontok">Erik Lontok</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Corse, Emily" sort="Corse, Emily" uniqKey="Corse E" first="Emily" last="Corse">Emily Corse</name>
</author>
<author><name sortKey="Machamer, Carolyn E" sort="Machamer, Carolyn E" uniqKey="Machamer C" first="Carolyn E" last="Machamer">Carolyn E. Machamer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15140989</idno>
<idno type="pmid">15140989</idno>
<idno type="doi">10.1128/JVI.78.11.5913-5922.2004</idno>
<idno type="wicri:Area/PubMed/Corpus">002E00</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002E00</idno>
<idno type="wicri:Area/PubMed/Curation">002E00</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002E00</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002C22</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002C22</idno>
<idno type="wicri:Area/Ncbi/Merge">000808</idno>
<idno type="wicri:Area/Ncbi/Curation">000808</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.</title>
<author><name sortKey="Lontok, Erik" sort="Lontok, Erik" uniqKey="Lontok E" first="Erik" last="Lontok">Erik Lontok</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Corse, Emily" sort="Corse, Emily" uniqKey="Corse E" first="Emily" last="Corse">Emily Corse</name>
</author>
<author><name sortKey="Machamer, Carolyn E" sort="Machamer, Carolyn E" uniqKey="Machamer C" first="Carolyn E" last="Machamer">Carolyn E. Machamer</name>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Amino Acid Sequence</term>
<term>Dipeptides</term>
<term>Endoplasmic Reticulum (physiology)</term>
<term>Golgi Apparatus (physiology)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Infectious bronchitis virus (physiology)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Molecular Sequence Data</term>
<term>Protein Transport</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (physiology)</term>
<term>Virus Assembly</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Appareil de Golgi (physiologie)</term>
<term>Assemblage viral</term>
<term>Cellules HeLa</term>
<term>Dipeptides</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Humains</term>
<term>Motifs d'acides aminés</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Réticulum endoplasmique (physiologie)</term>
<term>Séquence d'acides aminés</term>
<term>Transport de protéines</term>
<term>Virus de la bronchite infectieuse (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Dipeptides</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Appareil de Golgi</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Réticulum endoplasmique</term>
<term>Virus de la bronchite infectieuse</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Endoplasmic Reticulum</term>
<term>Golgi Apparatus</term>
<term>Infectious bronchitis virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Amino Acid Sequence</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Protein Transport</term>
<term>Virus Assembly</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Assemblage viral</term>
<term>Cellules HeLa</term>
<term>Dipeptides</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Humains</term>
<term>Motifs d'acides aminés</term>
<term>Protéines de l'enveloppe virale</term>
<term>Séquence d'acides aminés</term>
<term>Transport de protéines</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Coronavirus budding at the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) requires accumulation of the viral envelope proteins at this point in the secretory pathway. Here we demonstrate that the spike (S) protein from the group 3 coronavirus infectious bronchitis virus (IBV) contains a canonical dilysine endoplasmic reticulum retrieval signal (-KKXX-COOH) in its cytoplasmic tail. This signal can retain a chimeric reporter protein in the ERGIC and when mutated allows transport of the full-length S protein as well as the chimera to the plasma membrane. Interestingly, the IBV S protein also contains a tyrosine-based endocytosis signal in its cytoplasmic tail, suggesting that any S protein that escapes the ERGIC will be rapidly endocytosed when it reaches the plasma membrane. We also identified a novel dibasic motif (-KXHXX-COOH) in the cytoplasmic tails of S proteins from group 1 coronaviruses and from the newly identified coronavirus implicated in severe acute respiratory syndrome. This dibasic motif also retained a reporter protein in the ERGIC, similar to the dilysine motif in IBV S. The cytoplasmic tails of S proteins from group 2 coronaviruses lack an intracellular localization signal. The inherent differences in S-protein trafficking could point to interesting variations in pathogenesis of coronaviruses, since increased levels of surface S protein could promote syncytium formation and direct cell-to-cell spread of the infection.</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000808 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000808 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Ncbi
   |étape=   Curation
   |type=    RBID
   |clé=     pubmed:15140989
   |texte=   Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i   -Sk "pubmed:15140989" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.

Intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki