Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients
Identifieur interne : 000646 ( Ncbi/Curation ); précédent : 000645; suivant : 000647Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients
Auteurs : Ming Guan ; Hsiao Ying Chen ; Shen Yun Foo ; Yee-Joo Tan ; Phuay-Yee Goh ; Shock Hwa WeeSource :
- Clinical and Diagnostic Laboratory Immunology [ 1071-412X ] ; 2004.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (analyse), Anticorps antiviraux (immunologie), Humains, Immunoglobuline G (analyse), Immunoglobuline G (immunologie), Protéines recombinantes (immunologie), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Test ELISA (), Valeur prédictive des tests, Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : Anticorps antiviraux, Immunoglobuline G.
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Anticorps antiviraux, Immunoglobuline G, Protéines recombinantes, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Humains, Sensibilité et spécificité, Test ELISA, Valeur prédictive des tests.
English descriptors
- KwdEn :
- Antibodies, Viral (analysis), Antibodies, Viral (immunology), Enzyme-Linked Immunosorbent Assay (methods), Humans, Immunoglobulin G (analysis), Immunoglobulin G (immunology), Predictive Value of Tests, Recombinant Proteins (immunology), SARS Virus (immunology), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , analysis : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Antibodies, Viral, Immunoglobulin G, Recombinant Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Humans, Predictive Value of Tests, Sensitivity and Specificity.
Abstract
An enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic test for detection of immunoglobulin G (IgG) antibodies in severe acute respiratory syndrome (SARS) patients were developed by utilizing the well-characterized recombinant proteins Gst-N and Gst-U274. The ELISA detected IgG antibodies to SARS-CoV in all 74 convalescent-phase samples from SARS patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. This finding thus led to a kit sensitivity, specificity, and accuracy of 100, 99.5, and 99.6%, respectively. The test thus provided a positive predictive value (PPV) of 98.7% and a negative predictive value (NPV) of 100%. In addition, the ELISA gave a positive delta of 5.4 and a negative delta of 3.6, indicating an excellent differentiation between positives and negatives. The same recombinant proteins were also applied to a newly developed platform for the development of a 15-min rapid test. The resulting rapid test has an excellent agreement of 99.6%, with a kappa value of 1.00, with the ELISA. Again, this rapid test was able to detect 100% of the samples tested (
Url:
DOI: 10.1128/CDLI.11.2.287-291.2004
PubMed: 15013977
PubMed Central: 371224
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PMC:371224Le document en format XML
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Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients</title><author><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Yun Foo, Shen" sort="Yun Foo, Shen" uniqKey="Yun Foo S" first="Shen" last="Yun Foo">Shen Yun Foo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wee, Shock Hwa" sort="Wee, Shock Hwa" uniqKey="Wee S" first="Shock Hwa" last="Wee">Shock Hwa Wee</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Clinical and Diagnostic Laboratory Immunology</title><idno type="ISSN">1071-412X</idno><idno type="eISSN">1098-6588</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (analysis)</term><term>Antibodies, Viral (immunology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Immunoglobulin G (analysis)</term><term>Immunoglobulin G (immunology)</term><term>Predictive Value of Tests</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & 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qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term><term>Protéines recombinantes</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Predictive Value of Tests</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Sensibilité et spécificité</term><term>Test ELISA</term><term>Valeur prédictive des tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic test for detection of immunoglobulin G (IgG) antibodies in severe acute respiratory syndrome (SARS) patients were developed by utilizing the well-characterized recombinant proteins Gst-N and Gst-U274. The ELISA detected IgG antibodies to SARS-CoV in all 74 convalescent-phase samples from SARS patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. This finding thus led to a kit sensitivity, specificity, and accuracy of 100, 99.5, and 99.6%, respectively. The test thus provided a positive predictive value (PPV) of 98.7% and a negative predictive value (NPV) of 100%. In addition, the ELISA gave a positive delta of 5.4 and a negative delta of 3.6, indicating an excellent differentiation between positives and negatives. The same recombinant proteins were also applied to a newly developed platform for the development of a 15-min rapid test. The resulting rapid test has an excellent agreement of 99.6%, with a kappa value of 1.00, with the ELISA. Again, this rapid test was able to detect 100% of the samples tested (<italic>n</italic> = 42) while maintaining a specificity of 99.0% (<italic>n</italic> = 210). The PPV and NPV for the rapid test thus reached 95.3 and 100%, respectively.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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