Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients
Identifieur interne : 001545 ( Pmc/Checkpoint ); précédent : 001544; suivant : 001546Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients
Auteurs : Ming Guan ; Hsiao Ying Chen ; Shen Yun Foo ; Yee-Joo Tan ; Phuay-Yee Goh ; Shock Hwa WeeSource :
- Clinical and Diagnostic Laboratory Immunology [ 1071-412X ] ; 2004.
Abstract
An enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic test for detection of immunoglobulin G (IgG) antibodies in severe acute respiratory syndrome (SARS) patients were developed by utilizing the well-characterized recombinant proteins Gst-N and Gst-U274. The ELISA detected IgG antibodies to SARS-CoV in all 74 convalescent-phase samples from SARS patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. This finding thus led to a kit sensitivity, specificity, and accuracy of 100, 99.5, and 99.6%, respectively. The test thus provided a positive predictive value (PPV) of 98.7% and a negative predictive value (NPV) of 100%. In addition, the ELISA gave a positive delta of 5.4 and a negative delta of 3.6, indicating an excellent differentiation between positives and negatives. The same recombinant proteins were also applied to a newly developed platform for the development of a 15-min rapid test. The resulting rapid test has an excellent agreement of 99.6%, with a kappa value of 1.00, with the ELISA. Again, this rapid test was able to detect 100% of the samples tested (
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DOI: 10.1128/CDLI.11.2.287-291.2004
PubMed: 15013977
PubMed Central: 371224
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients</title><author><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Yun Foo, Shen" sort="Yun Foo, Shen" uniqKey="Yun Foo S" first="Shen" last="Yun Foo">Shen Yun Foo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wee, Shock Hwa" sort="Wee, Shock Hwa" uniqKey="Wee S" first="Shock Hwa" last="Wee">Shock Hwa Wee</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15013977</idno><idno type="pmc">371224</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC371224</idno><idno type="RBID">PMC:371224</idno><idno type="doi">10.1128/CDLI.11.2.287-291.2004</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000625</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000625</idno><idno type="wicri:Area/Pmc/Curation">000625</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000625</idno><idno type="wicri:Area/Pmc/Checkpoint">001545</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001545</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients</title><author><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Yun Foo, Shen" sort="Yun Foo, Shen" uniqKey="Yun Foo S" first="Shen" last="Yun Foo">Shen Yun Foo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wee, Shock Hwa" sort="Wee, Shock Hwa" uniqKey="Wee S" first="Shock Hwa" last="Wee">Shock Hwa Wee</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Clinical and Diagnostic Laboratory Immunology</title><idno type="ISSN">1071-412X</idno><idno type="eISSN">1098-6588</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic test for detection of immunoglobulin G (IgG) antibodies in severe acute respiratory syndrome (SARS) patients were developed by utilizing the well-characterized recombinant proteins Gst-N and Gst-U274. The ELISA detected IgG antibodies to SARS-CoV in all 74 convalescent-phase samples from SARS patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. This finding thus led to a kit sensitivity, specificity, and accuracy of 100, 99.5, and 99.6%, respectively. The test thus provided a positive predictive value (PPV) of 98.7% and a negative predictive value (NPV) of 100%. In addition, the ELISA gave a positive delta of 5.4 and a negative delta of 3.6, indicating an excellent differentiation between positives and negatives. The same recombinant proteins were also applied to a newly developed platform for the development of a 15-min rapid test. The resulting rapid test has an excellent agreement of 99.6%, with a kappa value of 1.00, with the ELISA. Again, this rapid test was able to detect 100% of the samples tested (<italic>n</italic> = 42) while maintaining a specificity of 99.0% (<italic>n</italic> = 210). The PPV and NPV for the rapid test thus reached 95.3 and 100%, respectively.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Diagn Lab Immunol</journal-id><journal-id journal-id-type="publisher-id">cdli</journal-id><journal-title>Clinical and Diagnostic Laboratory Immunology</journal-title><issn pub-type="ppub">1071-412X</issn><issn pub-type="epub">1098-6588</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15013977</article-id><article-id pub-id-type="pmc">371224</article-id><article-id pub-id-type="publisher-id">0270</article-id><article-id pub-id-type="doi">10.1128/CDLI.11.2.287-291.2004</article-id><article-categories><subj-group subj-group-type="heading"><subject>Microbial Immunology</subject></subj-group></article-categories><title-group><article-title>Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Guan</surname><given-names>Ming</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Hsiao Ying</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Yun Foo</surname><given-names>Shen</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tan</surname><given-names>Yee-Joo</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Goh</surname><given-names>Phuay-Yee</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Wee</surname><given-names>Shock Hwa</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib></contrib-group><aff id="aff1">Genelabs Diagnostics Pte, Ltd.,<label>1</label> Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore, Republic of Singapore<label>2</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Genelabs Diagnostics Pte Ltd., 85 Science Park Dr. 04-01, Singapore Science Park, Singapore 118259, Republic of Singapore. Phone: (65) 67750008. Fax: (65) 67754536. E-mail: <email>guanming@mail.genelabs.com.sg</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>3</month><year>2004</year></pub-date><volume>11</volume><issue>2</issue><fpage>287</fpage><lpage>291</lpage><history><date date-type="received"><day>10</day><month>11</month><year>2003</year></date><date date-type="rev-recd"><day>11</day><month>12</month><year>2003</year></date><date date-type="accepted"><day>29</day><month>12</month><year>2003</year></date></history><copyright-statement>Copyright © 2004, American Society for Microbiology</copyright-statement><copyright-year>2004</copyright-year><abstract><p>An enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic test for detection of immunoglobulin G (IgG) antibodies in severe acute respiratory syndrome (SARS) patients were developed by utilizing the well-characterized recombinant proteins Gst-N and Gst-U274. The ELISA detected IgG antibodies to SARS-CoV in all 74 convalescent-phase samples from SARS patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. This finding thus led to a kit sensitivity, specificity, and accuracy of 100, 99.5, and 99.6%, respectively. The test thus provided a positive predictive value (PPV) of 98.7% and a negative predictive value (NPV) of 100%. In addition, the ELISA gave a positive delta of 5.4 and a negative delta of 3.6, indicating an excellent differentiation between positives and negatives. The same recombinant proteins were also applied to a newly developed platform for the development of a 15-min rapid test. The resulting rapid test has an excellent agreement of 99.6%, with a kappa value of 1.00, with the ELISA. Again, this rapid test was able to detect 100% of the samples tested (<italic>n</italic> = 42) while maintaining a specificity of 99.0% (<italic>n</italic> = 210). The PPV and NPV for the rapid test thus reached 95.3 and 100%, respectively.</p></abstract></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chen, Hsiao Ying" sort="Chen, Hsiao Ying" uniqKey="Chen H" first="Hsiao Ying" last="Chen">Hsiao Ying Chen</name><name sortKey="Goh, Phuay Yee" sort="Goh, Phuay Yee" uniqKey="Goh P" first="Phuay-Yee" last="Goh">Phuay-Yee Goh</name><name sortKey="Guan, Ming" sort="Guan, Ming" uniqKey="Guan M" first="Ming" last="Guan">Ming Guan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Wee, Shock Hwa" sort="Wee, Shock Hwa" uniqKey="Wee S" first="Shock Hwa" last="Wee">Shock Hwa Wee</name><name sortKey="Yun Foo, Shen" sort="Yun Foo, Shen" uniqKey="Yun Foo S" first="Shen" last="Yun Foo">Shen Yun Foo</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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