Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
Identifieur interne : 000548 ( Ncbi/Curation ); précédent : 000547; suivant : 000549Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
Auteurs : Lisa F P. Ng ; Michelle Wong ; Susie Koh ; Eng-Eong Ooi ; King-Fai Tang ; Hoe-Nam Leong ; Ai-Ee Ling ; Lora V. Agathe ; Jenny Tan ; Edison T. Liu ; Ee-Chee Ren ; Lee-Ching Ng ; Martin L. HibberdSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Virus du SRAS.
- sang : ARN viral.
- virologie : Virémie.
- Humains, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : RNA, Viral.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- virology : Viremia.
- Humans.
Abstract
Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
DOI: 10.1128/jcm.42.1.347-350.2004
PubMed: 14715775
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Lisa F P. Ng<affiliation><nlm:affiliation>Genome Institute of Singapore, Singapore, Republic of Singapore.</nlm:affiliation><wicri:noCountry code="subField">Republic of Singapore</wicri:noCountry></affiliation>Le document en format XML
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Hibberd</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Viral (blood)</term><term>SARS Virus (isolation & purification)</term><term>Viremia (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (sang)</term><term>Humains</term><term>Réaction de polymérisation en chaîne ()</term><term>Virus du SRAS (isolement et purification)</term><term>Virémie (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Virémie</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Viremia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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