Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
Identifieur interne : 000548 ( Ncbi/Merge ); précédent : 000547; suivant : 000549Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
Auteurs : Lisa F P. Ng ; Michelle Wong ; Susie Koh ; Eng-Eong Ooi ; King-Fai Tang ; Hoe-Nam Leong ; Ai-Ee Ling ; Lora V. Agathe ; Jenny Tan ; Edison T. Liu ; Ee-Chee Ren ; Lee-Ching Ng ; Martin L. HibberdSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Virus du SRAS.
- sang : ARN viral.
- virologie : Virémie.
- Humains, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : RNA, Viral.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- virology : Viremia.
- Humans.
Abstract
Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
DOI: 10.1128/jcm.42.1.347-350.2004
PubMed: 14715775
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pubmed:14715775Le document en format XML
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Ng</name><affiliation><nlm:affiliation>Genome Institute of Singapore, Singapore, Republic of Singapore.</nlm:affiliation><wicri:noCountry code="subField">Republic of Singapore</wicri:noCountry></affiliation></author><author><name sortKey="Wong, Michelle" sort="Wong, Michelle" uniqKey="Wong M" first="Michelle" last="Wong">Michelle Wong</name></author><author><name sortKey="Koh, Susie" sort="Koh, Susie" uniqKey="Koh S" first="Susie" last="Koh">Susie Koh</name></author><author><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng-Eong" last="Ooi">Eng-Eong Ooi</name></author><author><name sortKey="Tang, King Fai" sort="Tang, King Fai" uniqKey="Tang K" first="King-Fai" last="Tang">King-Fai Tang</name></author><author><name sortKey="Leong, Hoe Nam" sort="Leong, Hoe Nam" uniqKey="Leong H" first="Hoe-Nam" last="Leong">Hoe-Nam Leong</name></author><author><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai-Ee" last="Ling">Ai-Ee Ling</name></author><author><name sortKey="Agathe, Lora V" sort="Agathe, Lora V" uniqKey="Agathe L" first="Lora V" last="Agathe">Lora V. 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Ng</name><affiliation><nlm:affiliation>Genome Institute of Singapore, Singapore, Republic of Singapore.</nlm:affiliation><wicri:noCountry code="subField">Republic of Singapore</wicri:noCountry></affiliation></author><author><name sortKey="Wong, Michelle" sort="Wong, Michelle" uniqKey="Wong M" first="Michelle" last="Wong">Michelle Wong</name></author><author><name sortKey="Koh, Susie" sort="Koh, Susie" uniqKey="Koh S" first="Susie" last="Koh">Susie Koh</name></author><author><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng-Eong" last="Ooi">Eng-Eong Ooi</name></author><author><name sortKey="Tang, King Fai" sort="Tang, King Fai" uniqKey="Tang K" first="King-Fai" last="Tang">King-Fai Tang</name></author><author><name sortKey="Leong, Hoe Nam" sort="Leong, Hoe Nam" uniqKey="Leong H" first="Hoe-Nam" last="Leong">Hoe-Nam Leong</name></author><author><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai-Ee" last="Ling">Ai-Ee Ling</name></author><author><name sortKey="Agathe, Lora V" sort="Agathe, Lora V" uniqKey="Agathe L" first="Lora V" last="Agathe">Lora V. Agathe</name></author><author><name sortKey="Tan, Jenny" sort="Tan, Jenny" uniqKey="Tan J" first="Jenny" last="Tan">Jenny Tan</name></author><author><name sortKey="Liu, Edison T" sort="Liu, Edison T" uniqKey="Liu E" first="Edison T" last="Liu">Edison T. Liu</name></author><author><name sortKey="Ren, Ee Chee" sort="Ren, Ee Chee" uniqKey="Ren E" first="Ee-Chee" last="Ren">Ee-Chee Ren</name></author><author><name sortKey="Ng, Lee Ching" sort="Ng, Lee Ching" uniqKey="Ng L" first="Lee-Ching" last="Ng">Lee-Ching Ng</name></author><author><name sortKey="Hibberd, Martin L" sort="Hibberd, Martin L" uniqKey="Hibberd M" first="Martin L" last="Hibberd">Martin L. Hibberd</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Viral (blood)</term><term>SARS Virus (isolation & purification)</term><term>Viremia (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (sang)</term><term>Humains</term><term>Réaction de polymérisation en chaîne ()</term><term>Virus du SRAS (isolement et purification)</term><term>Virémie (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Virémie</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Viremia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14715775</PMID><DateCompleted><Year>2004</Year><Month>03</Month><Day>05</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.</ArticleTitle><Pagination><MedlinePgn>347-50</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>Lisa F P</ForeName><Initials>LF</Initials><AffiliationInfo><Affiliation>Genome Institute of Singapore, Singapore, Republic of Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wong</LastName><ForeName>Michelle</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Koh</LastName><ForeName>Susie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ooi</LastName><ForeName>Eng-Eong</ForeName><Initials>EE</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>King-Fai</ForeName><Initials>KF</Initials></Author><Author ValidYN="Y"><LastName>Leong</LastName><ForeName>Hoe-Nam</ForeName><Initials>HN</Initials></Author><Author ValidYN="Y"><LastName>Ling</LastName><ForeName>Ai-Ee</ForeName><Initials>AE</Initials></Author><Author ValidYN="Y"><LastName>Agathe</LastName><ForeName>Lora V</ForeName><Initials>LV</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Jenny</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Edison T</ForeName><Initials>ET</Initials></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Ee-Chee</ForeName><Initials>EC</Initials></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>Lee-Ching</ForeName><Initials>LC</Initials></Author><Author ValidYN="Y"><LastName>Hibberd</LastName><ForeName>Martin L</ForeName><Initials>ML</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName 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Agathe</name><name sortKey="Hibberd, Martin L" sort="Hibberd, Martin L" uniqKey="Hibberd M" first="Martin L" last="Hibberd">Martin L. Hibberd</name><name sortKey="Koh, Susie" sort="Koh, Susie" uniqKey="Koh S" first="Susie" last="Koh">Susie Koh</name><name sortKey="Leong, Hoe Nam" sort="Leong, Hoe Nam" uniqKey="Leong H" first="Hoe-Nam" last="Leong">Hoe-Nam Leong</name><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai-Ee" last="Ling">Ai-Ee Ling</name><name sortKey="Liu, Edison T" sort="Liu, Edison T" uniqKey="Liu E" first="Edison T" last="Liu">Edison T. Liu</name><name sortKey="Ng, Lee Ching" sort="Ng, Lee Ching" uniqKey="Ng L" first="Lee-Ching" last="Ng">Lee-Ching Ng</name><name sortKey="Ng, Lisa F P" sort="Ng, Lisa F P" uniqKey="Ng L" first="Lisa F P" last="Ng">Lisa F P. Ng</name><name sortKey="Ooi, Eng Eong" sort="Ooi, Eng Eong" uniqKey="Ooi E" first="Eng-Eong" last="Ooi">Eng-Eong Ooi</name><name sortKey="Ren, Ee Chee" sort="Ren, Ee Chee" uniqKey="Ren E" first="Ee-Chee" last="Ren">Ee-Chee Ren</name><name sortKey="Tan, Jenny" sort="Tan, Jenny" uniqKey="Tan J" first="Jenny" last="Tan">Jenny Tan</name><name sortKey="Tang, King Fai" sort="Tang, King Fai" uniqKey="Tang K" first="King-Fai" last="Tang">King-Fai Tang</name><name sortKey="Wong, Michelle" sort="Wong, Michelle" uniqKey="Wong M" first="Michelle" last="Wong">Michelle Wong</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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