SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.
Identifieur interne : 002436 ( Ncbi/Checkpoint ); précédent : 002435; suivant : 002437SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.
Auteurs : Wei-Yen Wei [Taïwan] ; Hui-Chun Li ; Chiung-Yao Chen ; Chee-Hing Yang ; Shen-Kao Lee ; Chia-Wen Wang ; Hsin-Chieh Ma ; Yue-Li Juang ; Shih-Yen LoSource :
- Archives of virology [ 1432-8798 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Cartographie d'interactions entre protéines, Humains, Immunoprécipitation, Interactions hôte-pathogène, Liaison aux protéines, Lignée cellulaire, Microscopie confocale, Motifs et domaines d'intéraction protéique, Protéines nucléocapside (métabolisme), Pyruvate kinase (antagonistes et inhibiteurs), Techniques de double hybride, Virus du SRAS (pathogénicité).
- MESH :
- antagonistes et inhibiteurs : Pyruvate kinase.
- métabolisme : Protéines nucléocapside.
- pathogénicité : Virus du SRAS.
- Animaux, Cartographie d'interactions entre protéines, Humains, Immunoprécipitation, Interactions hôte-pathogène, Liaison aux protéines, Lignée cellulaire, Microscopie confocale, Motifs et domaines d'intéraction protéique, Techniques de double hybride.
English descriptors
- KwdEn :
- Animals, Cell Line, Host-Pathogen Interactions, Humans, Immunoprecipitation, Microscopy, Confocal, Nucleocapsid Proteins (metabolism), Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Pyruvate Kinase (antagonists & inhibitors), SARS Virus (pathogenicity), Two-Hybrid System Techniques.
- MESH :
- chemical , antagonists & inhibitors : Pyruvate Kinase.
- chemical , metabolism : Nucleocapsid Proteins.
- pathogenicity : SARS Virus.
- Animals, Cell Line, Host-Pathogen Interactions, Humans, Immunoprecipitation, Microscopy, Confocal, Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Two-Hybrid System Techniques.
Abstract
The pathogenesis of SARS-CoV remains largely unknown. To study the function of the SARS-CoV nucleocapsid protein, we have conducted a yeast two-hybrid screening experiment to identify cellular proteins that may interact with the SARS-CoV nucleocapsid protein. Pyruvate kinase (liver) was found to interact with SARS-CoV nucleocapsid protein in this experiment. The binding domains of these two proteins were also determined using the yeast two-hybrid system. The physical interaction between the SARS-CoV nucleocapsid and cellular pyruvate kinase (liver) proteins was further confirmed by GST pull-down assay, co-immunoprecipitation assay and confocal microscopy. Cellular pyruvate kinase activity in hepatoma cells was repressed by SARS-CoV nucleocapsid protein in either transiently transfected or stably transfected cells. PK deficiency in red blood cells is known to result in human hereditary non-spherocytic hemolytic anemia. It is reasonable to assume that an inhibition of PKL activity due to interaction with SARS-CoV N protein is likely to cause the death of the hepatocytes, which results in the elevation of serum alanine aminotransferase and liver dysfunction noted in most SARS patients. Thus, our results suggest that SARS-CoV could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease.
DOI: 10.1007/s00705-011-1221-7
PubMed: 22222284
Affiliations:
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pubmed:22222284Le document en format XML
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xml:lang="fr"><term>Pyruvate kinase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Immunoprecipitation</term><term>Microscopy, Confocal</term><term>Protein Binding</term><term>Protein Interaction Domains and Motifs</term><term>Protein Interaction Mapping</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartographie d'interactions entre protéines</term><term>Humains</term><term>Immunoprécipitation</term><term>Interactions hôte-pathogène</term><term>Liaison aux protéines</term><term>Lignée 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To study the function of the SARS-CoV nucleocapsid protein, we have conducted a yeast two-hybrid screening experiment to identify cellular proteins that may interact with the SARS-CoV nucleocapsid protein. Pyruvate kinase (liver) was found to interact with SARS-CoV nucleocapsid protein in this experiment. The binding domains of these two proteins were also determined using the yeast two-hybrid system. The physical interaction between the SARS-CoV nucleocapsid and cellular pyruvate kinase (liver) proteins was further confirmed by GST pull-down assay, co-immunoprecipitation assay and confocal microscopy. Cellular pyruvate kinase activity in hepatoma cells was repressed by SARS-CoV nucleocapsid protein in either transiently transfected or stably transfected cells. PK deficiency in red blood cells is known to result in human hereditary non-spherocytic hemolytic anemia. It is reasonable to assume that an inhibition of PKL activity due to interaction with SARS-CoV N protein is likely to cause the death of the hepatocytes, which results in the elevation of serum alanine aminotransferase and liver dysfunction noted in most SARS patients. Thus, our results suggest that SARS-CoV could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chen, Chiung Yao" sort="Chen, Chiung Yao" uniqKey="Chen C" first="Chiung-Yao" last="Chen">Chiung-Yao Chen</name><name sortKey="Juang, Yue Li" sort="Juang, Yue Li" uniqKey="Juang Y" first="Yue-Li" last="Juang">Yue-Li Juang</name><name sortKey="Lee, Shen Kao" sort="Lee, Shen Kao" uniqKey="Lee S" first="Shen-Kao" last="Lee">Shen-Kao Lee</name><name sortKey="Li, Hui Chun" sort="Li, Hui Chun" uniqKey="Li H" first="Hui-Chun" last="Li">Hui-Chun Li</name><name sortKey="Lo, Shih Yen" sort="Lo, Shih Yen" uniqKey="Lo S" first="Shih-Yen" last="Lo">Shih-Yen Lo</name><name sortKey="Ma, Hsin Chieh" sort="Ma, Hsin Chieh" uniqKey="Ma H" first="Hsin-Chieh" last="Ma">Hsin-Chieh Ma</name><name sortKey="Wang, Chia Wen" sort="Wang, Chia Wen" uniqKey="Wang C" first="Chia-Wen" last="Wang">Chia-Wen Wang</name><name sortKey="Yang, Chee Hing" sort="Yang, Chee Hing" uniqKey="Yang C" first="Chee-Hing" last="Yang">Chee-Hing Yang</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Wei, Wei Yen" sort="Wei, Wei Yen" uniqKey="Wei W" first="Wei-Yen" last="Wei">Wei-Yen Wei</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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