SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.
Identifieur interne : 001421 ( PubMed/Curation ); précédent : 001420; suivant : 001422SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.
Auteurs : Wei-Yen Wei [Taïwan] ; Hui-Chun Li ; Chiung-Yao Chen ; Chee-Hing Yang ; Shen-Kao Lee ; Chia-Wen Wang ; Hsin-Chieh Ma ; Yue-Li Juang ; Shih-Yen LoSource :
- Archives of virology [ 1432-8798 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Cartographie d'interactions entre protéines, Humains, Immunoprécipitation, Interactions hôte-pathogène, Liaison aux protéines, Lignée cellulaire, Microscopie confocale, Motifs et domaines d'intéraction protéique, Protéines nucléocapside (métabolisme), Pyruvate kinase (antagonistes et inhibiteurs), Techniques de double hybride, Virus du SRAS (pathogénicité).
- MESH :
- antagonistes et inhibiteurs : Pyruvate kinase.
- métabolisme : Protéines nucléocapside.
- pathogénicité : Virus du SRAS.
- Animaux, Cartographie d'interactions entre protéines, Humains, Immunoprécipitation, Interactions hôte-pathogène, Liaison aux protéines, Lignée cellulaire, Microscopie confocale, Motifs et domaines d'intéraction protéique, Techniques de double hybride.
English descriptors
- KwdEn :
- Animals, Cell Line, Host-Pathogen Interactions, Humans, Immunoprecipitation, Microscopy, Confocal, Nucleocapsid Proteins (metabolism), Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Pyruvate Kinase (antagonists & inhibitors), SARS Virus (pathogenicity), Two-Hybrid System Techniques.
- MESH :
- chemical , antagonists & inhibitors : Pyruvate Kinase.
- chemical , metabolism : Nucleocapsid Proteins.
- pathogenicity : SARS Virus.
- Animals, Cell Line, Host-Pathogen Interactions, Humans, Immunoprecipitation, Microscopy, Confocal, Protein Binding, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Two-Hybrid System Techniques.
Abstract
The pathogenesis of SARS-CoV remains largely unknown. To study the function of the SARS-CoV nucleocapsid protein, we have conducted a yeast two-hybrid screening experiment to identify cellular proteins that may interact with the SARS-CoV nucleocapsid protein. Pyruvate kinase (liver) was found to interact with SARS-CoV nucleocapsid protein in this experiment. The binding domains of these two proteins were also determined using the yeast two-hybrid system. The physical interaction between the SARS-CoV nucleocapsid and cellular pyruvate kinase (liver) proteins was further confirmed by GST pull-down assay, co-immunoprecipitation assay and confocal microscopy. Cellular pyruvate kinase activity in hepatoma cells was repressed by SARS-CoV nucleocapsid protein in either transiently transfected or stably transfected cells. PK deficiency in red blood cells is known to result in human hereditary non-spherocytic hemolytic anemia. It is reasonable to assume that an inhibition of PKL activity due to interaction with SARS-CoV N protein is likely to cause the death of the hepatocytes, which results in the elevation of serum alanine aminotransferase and liver dysfunction noted in most SARS patients. Thus, our results suggest that SARS-CoV could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease.
DOI: 10.1007/s00705-011-1221-7
PubMed: 22222284
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domaines d'intéraction protéique</term><term>Protéines nucléocapside (métabolisme)</term><term>Pyruvate kinase (antagonistes et inhibiteurs)</term><term>Techniques de double hybride</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Pyruvate Kinase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Pyruvate kinase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Immunoprecipitation</term><term>Microscopy, Confocal</term><term>Protein Binding</term><term>Protein Interaction Domains and Motifs</term><term>Protein Interaction Mapping</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartographie d'interactions entre protéines</term><term>Humains</term><term>Immunoprécipitation</term><term>Interactions hôte-pathogène</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Microscopie confocale</term><term>Motifs et domaines d'intéraction protéique</term><term>Techniques de double hybride</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathogenesis of SARS-CoV remains largely unknown. To study the function of the SARS-CoV nucleocapsid protein, we have conducted a yeast two-hybrid screening experiment to identify cellular proteins that may interact with the SARS-CoV nucleocapsid protein. Pyruvate kinase (liver) was found to interact with SARS-CoV nucleocapsid protein in this experiment. The binding domains of these two proteins were also determined using the yeast two-hybrid system. The physical interaction between the SARS-CoV nucleocapsid and cellular pyruvate kinase (liver) proteins was further confirmed by GST pull-down assay, co-immunoprecipitation assay and confocal microscopy. Cellular pyruvate kinase activity in hepatoma cells was repressed by SARS-CoV nucleocapsid protein in either transiently transfected or stably transfected cells. PK deficiency in red blood cells is known to result in human hereditary non-spherocytic hemolytic anemia. It is reasonable to assume that an inhibition of PKL activity due to interaction with SARS-CoV N protein is likely to cause the death of the hepatocytes, which results in the elevation of serum alanine aminotransferase and liver dysfunction noted in most SARS patients. Thus, our results suggest that SARS-CoV could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22222284</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-8798</ISSN><JournalIssue CitedMedium="Internet"><Volume>157</Volume><Issue>4</Issue><PubDate><Year>2012</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Archives of virology</Title><ISOAbbreviation>Arch. Virol.</ISOAbbreviation></Journal><ArticleTitle>SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.</ArticleTitle><Pagination><MedlinePgn>635-45</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00705-011-1221-7</ELocationID><Abstract><AbstractText>The pathogenesis of SARS-CoV remains largely unknown. To study the function of the SARS-CoV nucleocapsid protein, we have conducted a yeast two-hybrid screening experiment to identify cellular proteins that may interact with the SARS-CoV nucleocapsid protein. Pyruvate kinase (liver) was found to interact with SARS-CoV nucleocapsid protein in this experiment. The binding domains of these two proteins were also determined using the yeast two-hybrid system. The physical interaction between the SARS-CoV nucleocapsid and cellular pyruvate kinase (liver) proteins was further confirmed by GST pull-down assay, co-immunoprecipitation assay and confocal microscopy. Cellular pyruvate kinase activity in hepatoma cells was repressed by SARS-CoV nucleocapsid protein in either transiently transfected or stably transfected cells. PK deficiency in red blood cells is known to result in human hereditary non-spherocytic hemolytic anemia. It is reasonable to assume that an inhibition of PKL activity due to interaction with SARS-CoV N protein is likely to cause the death of the hepatocytes, which results in the elevation of serum alanine aminotransferase and liver dysfunction noted in most SARS patients. Thus, our results suggest that SARS-CoV could reduce pyruvate kinase activity via its nucleocapsid protein, and this may in turn cause disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Wei-Yen</ForeName><Initials>WY</Initials><AffiliationInfo><Affiliation>Graduate Institute of Molecular and Cellular Biology, Tzu Chi University, Hualien, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hui-Chun</ForeName><Initials>HC</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Chiung-Yao</ForeName><Initials>CY</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chee-Hing</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Shen-Kao</ForeName><Initials>SK</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Chia-Wen</ForeName><Initials>CW</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Hsin-Chieh</ForeName><Initials>HC</Initials></Author><Author ValidYN="Y"><LastName>Juang</LastName><ForeName>Yue-Li</ForeName><Initials>YL</Initials></Author><Author ValidYN="Y"><LastName>Lo</LastName><ForeName>Shih-Yen</ForeName><Initials>SY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>01</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Arch Virol</MedlineTA><NlmUniqueID>7506870</NlmUniqueID><ISSNLinking>0304-8608</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 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IdType="pubmed">12702616</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1291-6</Citation><ArticleIdList><ArticleId IdType="pubmed">9990017</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Gastroenterol. 2005 Jan;100(1):169-76</Citation><ArticleIdList><ArticleId IdType="pubmed">15654797</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Microbes Infect. 2007 Jan;9(1):119-26</Citation><ArticleIdList><ArticleId IdType="pubmed">17142081</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Hepatology. 2004 Feb;39(2):302-10</Citation><ArticleIdList><ArticleId IdType="pubmed">14767982</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Biol Cell. 2010 Nov 15;21(22):3838-52</Citation><ArticleIdList><ArticleId IdType="pubmed">20861307</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 1999 Apr 9;274(15):9930-6</Citation><ArticleIdList><ArticleId IdType="pubmed">10187767</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1351</Citation><ArticleIdList><ArticleId IdType="pubmed">12775803</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2010 May 17;7:99</Citation><ArticleIdList><ArticleId IdType="pubmed">20478047</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2008 Feb 8;283(6):3272-80</Citation><ArticleIdList><ArticleId IdType="pubmed">18055455</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol Sin. 2010 Oct;25(5):369-74</Citation><ArticleIdList><ArticleId IdType="pubmed">20960183</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>World J Gastroenterol. 2005 Apr 14;11(14):2148-53</Citation><ArticleIdList><ArticleId IdType="pubmed">15810082</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2005 Jan 15;191(2):193-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15609228</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Genet Evol. 2008 Jul;8(4):397-405</Citation><ArticleIdList><ArticleId IdType="pubmed">17881296</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biomed Sci. 2003 Nov-Dec;10(6 Pt 2):664-75</Citation><ArticleIdList><ArticleId IdType="pubmed">14631105</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biomed Sci. 2010 Aug 04;17:65</Citation><ArticleIdList><ArticleId IdType="pubmed">20682079</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Trop Med Hyg. 2004 Aug;71(2):125-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15306699</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet Infect Dis. 2004 Nov;4(11):663-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15522678</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2005 Feb;65(2):69-78</Citation><ArticleIdList><ArticleId IdType="pubmed">15708633</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Comp Biochem Physiol B Biochem Mol Biol. 2003 Jun;135(2):197-218</Citation><ArticleIdList><ArticleId IdType="pubmed">12798932</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2005 Jun 17;280(24):23280-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15849181</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochemistry. 2005 Nov 22;44(46):15351-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16285739</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2009 May 25;388(1):112-20</Citation><ArticleIdList><ArticleId IdType="pubmed">19345973</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Pathol. 2003 Jul;200(3):282-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12845623</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 May 14;317(4):1030-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15094372</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Exp Med. 2005 Aug 1;202(3):415-24</Citation><ArticleIdList><ArticleId IdType="pubmed">16043521</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol Methods. 2005 Nov;129(2):152-61</Citation><ArticleIdList><ArticleId IdType="pubmed">16023741</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Jun;78(12):6134-42</Citation><ArticleIdList><ArticleId IdType="pubmed">15163706</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Hepatobiliary Pancreat Dis Int. 2005 Feb;4(1):60-3</Citation><ArticleIdList><ArticleId IdType="pubmed">15730921</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Brain Res. 2004 Jun 25;1012(1-2):93-100</Citation><ArticleIdList><ArticleId IdType="pubmed">15158165</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2003 Nov 28;311(4):870-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14623261</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2008 Apr;133(1):4-12</Citation><ArticleIdList><ArticleId IdType="pubmed">17825937</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Pathology. 2003 Dec;35(6):526-31</Citation><ArticleIdList><ArticleId IdType="pubmed">14660106</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Genes. 2009 Jun;38(3):365-71</Citation><ArticleIdList><ArticleId IdType="pubmed">19322648</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biomed Sci. 2008 Jul;15(4):417-25</Citation><ArticleIdList><ArticleId IdType="pubmed">18398700</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Ann N Y Acad Sci. 2007 Apr;1102:26-38</Citation><ArticleIdList><ArticleId IdType="pubmed">17470909</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biomed Sci. 2008 May;15(3):301-10</Citation><ArticleIdList><ArticleId IdType="pubmed">18398701</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2002 Jun 28;277(26):23807-14</Citation><ArticleIdList><ArticleId IdType="pubmed">11960989</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Structure. 1998 Feb 15;6(2):195-210</Citation><ArticleIdList><ArticleId IdType="pubmed">9519410</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2008 Jun 25;582(15):2155-60</Citation><ArticleIdList><ArticleId IdType="pubmed">18519040</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2005 Dec;86(Pt 12):3303-3310</Citation><ArticleIdList><ArticleId IdType="pubmed">16298975</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2008 Jul;82(14):6962-71</Citation><ArticleIdList><ArticleId IdType="pubmed">18448518</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem J. 2004 Oct 1;383(Pt 1):13-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15294014</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Disord Drug Targets. 2007 Mar;7(1):29-41</Citation><ArticleIdList><ArticleId IdType="pubmed">17346209</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Blood. 2001 Nov 15;98(10):3113-20</Citation><ArticleIdList><ArticleId IdType="pubmed">11698298</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 1988 Dec 19;242(1):194-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2462512</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>World J Gastroenterol. 2004 Jun 1;10(11):1652-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15162543</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2010 Apr 08;6(4):e1000849</Citation><ArticleIdList><ArticleId IdType="pubmed">20386712</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2007 Sep 1;365(2):324-35</Citation><ArticleIdList><ArticleId IdType="pubmed">17490702</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2010 Jul 5;402(2):281-91</Citation><ArticleIdList><ArticleId IdType="pubmed">20409569</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Comp Pathol. 2008 May;138(4):171-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18343398</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biomed Sci. 2008 Nov;15(6):707-17</Citation><ArticleIdList><ArticleId IdType="pubmed">18792806</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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