Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation.
Identifieur interne : 002390 ( Ncbi/Checkpoint ); précédent : 002389; suivant : 002391Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation.
Auteurs : Xiaoqing Chen [République populaire de Chine] ; Zhongxia Wang ; Ziying Yang ; Jingjing Wang ; Yunxia Xu ; Ren-Xiang Tan ; Erguang LiSource :
- Antiviral research [ 1872-9096 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (isolement et purification), Antiviraux (pharmacologie), Cellules HeLa, Cellules Vero, Charge virale, Concentration inhibitrice 50, Extraits de plantes (isolement et purification), Extraits de plantes (pharmacologie), Facteur de transcription NF-kappa B (antagonistes et inhibiteurs), Herpèsvirus humain de type 2 (croissance et développement), Houttuynia (), Humains, Méthode des plages virales, Réplication virale ().
- MESH :
- antagonistes et inhibiteurs : Facteur de transcription NF-kappa B.
- croissance et développement : Herpèsvirus humain de type 2.
- isolement et purification : Antiviraux, Extraits de plantes.
- pharmacologie : Antiviraux, Extraits de plantes.
- Animaux, Cellules HeLa, Cellules Vero, Charge virale, Concentration inhibitrice 50, Houttuynia, Humains, Méthode des plages virales, Réplication virale.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (isolation & purification), Antiviral Agents (pharmacology), Chlorocebus aethiops, HeLa Cells, Herpesvirus 2, Human (growth & development), Houttuynia (chemistry), Humans, Inhibitory Concentration 50, NF-kappa B (antagonists & inhibitors), Plant Extracts (isolation & purification), Plant Extracts (pharmacology), Vero Cells, Viral Load, Viral Plaque Assay, Virus Replication (drug effects).
- MESH :
- chemical , antagonists & inhibitors : NF-kappa B.
- chemical , isolation & purification : Antiviral Agents, Plant Extracts.
- chemical , pharmacology : Antiviral Agents, Plant Extracts.
- chemistry : Houttuynia.
- drug effects : Virus Replication.
- growth & development : Herpesvirus 2, Human.
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Inhibitory Concentration 50, Vero Cells, Viral Load, Viral Plaque Assay.
Abstract
Houttuynia cordata Thunb. is a medicinal plant widely used in folk medicine in several Asian countries. It has been reported that a water extract of H. cordata exhibits activity against herpes simplex virus (HSV) and the virus of severe acute respiratory syndrome (SARS), although the mechanisms are not fully understood yet. Previous studies have demonstrated absolute requirement of NF-κB activation for efficient replication of HSV-1 and HSV-2 and inhibition of NF-κB activation has been shown to suppress HSV infection. Here we show that a hot water extract of H. cordata (HCWE) inhibits HSV-2 infection through inhibition of NF-κB activation. The IC(50) was estimated at 50 μg/ml of lyophilized HCWE powder. At 150 and 450 μg/ml, HCWE blocked infectious HSV-2 production by more than 3 and 4 logs, respectively. The inhibitory activity was concomitant with an inhibition of NF-κB activation by HSV-2 infection. Although activation of NF-κB and Erk MAPK has been implicated for HSV replication and growth, HCWE showed no effect on HSV-2-induced Erk activation. Furthermore, we show that treatment with quercetin, quercitrin or isoquercitrin, major water extractable flavonoids from H. cordata, significantly blocked HSV-2 infection. These results together demonstrated that H. cordata blocks HSV-2 infection through inhibition of NF-κB activation.
DOI: 10.1016/j.antiviral.2011.09.005
PubMed: 21951655
Affiliations:
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pubmed:21951655Le document en format XML
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Vero</term><term>Charge virale</term><term>Concentration inhibitrice 50</term><term>Extraits de plantes (isolement et purification)</term><term>Extraits de plantes (pharmacologie)</term><term>Facteur de transcription NF-kappa B (antagonistes et inhibiteurs)</term><term>Herpèsvirus humain de type 2 (croissance et développement)</term><term>Houttuynia ()</term><term>Humains</term><term>Méthode des plages virales</term><term>Réplication virale ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>NF-kappa B</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiviral Agents</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Facteur de transcription NF-kappa B</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Houttuynia</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Herpèsvirus humain de type 2</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Herpesvirus 2, Human</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antiviraux</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>HeLa Cells</term><term>Humans</term><term>Inhibitory Concentration 50</term><term>Vero Cells</term><term>Viral Load</term><term>Viral Plaque Assay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Charge virale</term><term>Concentration inhibitrice 50</term><term>Houttuynia</term><term>Humains</term><term>Méthode des plages virales</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Houttuynia cordata Thunb. is a medicinal plant widely used in folk medicine in several Asian countries. It has been reported that a water extract of H. cordata exhibits activity against herpes simplex virus (HSV) and the virus of severe acute respiratory syndrome (SARS), although the mechanisms are not fully understood yet. Previous studies have demonstrated absolute requirement of NF-κB activation for efficient replication of HSV-1 and HSV-2 and inhibition of NF-κB activation has been shown to suppress HSV infection. Here we show that a hot water extract of H. cordata (HCWE) inhibits HSV-2 infection through inhibition of NF-κB activation. The IC(50) was estimated at 50 μg/ml of lyophilized HCWE powder. At 150 and 450 μg/ml, HCWE blocked infectious HSV-2 production by more than 3 and 4 logs, respectively. The inhibitory activity was concomitant with an inhibition of NF-κB activation by HSV-2 infection. Although activation of NF-κB and Erk MAPK has been implicated for HSV replication and growth, HCWE showed no effect on HSV-2-induced Erk activation. Furthermore, we show that treatment with quercetin, quercitrin or isoquercitrin, major water extractable flavonoids from H. cordata, significantly blocked HSV-2 infection. These results together demonstrated that H. cordata blocks HSV-2 infection through inhibition of NF-κB activation.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Li, Erguang" sort="Li, Erguang" uniqKey="Li E" first="Erguang" last="Li">Erguang Li</name><name sortKey="Tan, Ren Xiang" sort="Tan, Ren Xiang" uniqKey="Tan R" first="Ren-Xiang" last="Tan">Ren-Xiang Tan</name><name sortKey="Wang, Jingjing" sort="Wang, Jingjing" uniqKey="Wang J" first="Jingjing" last="Wang">Jingjing Wang</name><name sortKey="Wang, Zhongxia" sort="Wang, Zhongxia" uniqKey="Wang Z" first="Zhongxia" last="Wang">Zhongxia Wang</name><name sortKey="Xu, Yunxia" sort="Xu, Yunxia" uniqKey="Xu Y" first="Yunxia" last="Xu">Yunxia Xu</name><name sortKey="Yang, Ziying" sort="Yang, Ziying" uniqKey="Yang Z" first="Ziying" last="Yang">Ziying Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Xiaoqing" sort="Chen, Xiaoqing" uniqKey="Chen X" first="Xiaoqing" last="Chen">Xiaoqing Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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