A second, non‐canonical RNA‐dependent RNA polymerase in SARS Coronavirus
Identifieur interne : 001680 ( Ncbi/Checkpoint ); précédent : 001679; suivant : 001681A second, non‐canonical RNA‐dependent RNA polymerase in SARS Coronavirus
Auteurs : Isabelle Imbert ; Jean-Claude Guillemot ; Jean-Marie Bourhis ; Cécile Bussetta ; Bruno Coutard ; Marie-Pierre Egloff ; François Ferron ; Alexander E. Gorbalenya ; Bruno CanardSource :
- The EMBO Journal [ 0261-4189 ] ; 2006.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (biosynthèse), ARN viral (génétique), Domaine catalytique (génétique), Données de séquences moléculaires, Modèles moléculaires, Protéines virales (), Protéines virales (génétique), Protéines virales (métabolisme), RNA replicase (), RNA replicase (génétique), RNA replicase (métabolisme), Structure tertiaire des protéines (génétique), Séquence d'acides aminés, Virus du SRAS (), Virus du SRAS (enzymologie), Virus du SRAS (génétique).
- MESH :
- biosynthèse : ARN viral.
- enzymologie : Virus du SRAS.
- génétique : ARN viral, Domaine catalytique, Protéines virales, RNA replicase, Structure tertiaire des protéines, Virus du SRAS.
- métabolisme : Protéines virales, RNA replicase.
- ARN viral, Données de séquences moléculaires, Modèles moléculaires, Protéines virales, RNA replicase, Séquence d'acides aminés, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Catalytic Domain (genetics), Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary (genetics), RNA Replicase (chemistry), RNA Replicase (genetics), RNA Replicase (metabolism), RNA, Viral (biosynthesis), RNA, Viral (chemistry), RNA, Viral (genetics), SARS Virus (chemistry), SARS Virus (enzymology), SARS Virus (genetics), Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , chemistry : RNA Replicase, RNA, Viral, Viral Proteins.
- chemistry : SARS Virus.
- enzymology : SARS Virus.
- genetics : Catalytic Domain, Protein Structure, Tertiary, RNA Replicase, RNA, Viral, SARS Virus, Viral Proteins.
- chemical , metabolism : RNA Replicase, Viral Proteins.
- Amino Acid Sequence, Models, Molecular, Molecular Sequence Data.
Abstract
In (+) RNA coronaviruses, replication and transcription of the giant ∼30 kb genome to produce genome‐ and subgenome‐size RNAs of both polarities are mediated by a cognate membrane‐bound enzymatic complex. Its RNA‐dependent RNA polymerase (RdRp) activity appears to be supplied by non‐structural protein 12 (nsp12) that includes an RdRp domain conserved in all RNA viruses. Using SARS coronavirus, we now show that coronaviruses uniquely encode a second RdRp residing in nsp8. This protein strongly prefers the internal 5′‐(G/U)CC‐3′ trinucleotides on RNA templates to initiate the synthesis of complementary oligonucleotides of <6 residues in a reaction whose fidelity is relatively low. Distant structural homology between the C‐terminal domain of nsp8 and the catalytic palm subdomain of RdRps of RNA viruses suggests a common origin of the two coronavirus RdRps, which however may have evolved different sets of catalytic residues. A parallel between the nsp8 RdRp and cellular DNA‐dependent RNA primases is drawn to propose that the nsp8 RdRp produces primers utilized by the primer‐dependent nsp12 RdRp.
Url:
DOI: 10.1038/sj.emboj.7601368
PubMed: 17024178
PubMed Central: 1618104
Affiliations:
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Its RNA‐dependent RNA polymerase (RdRp) activity appears to be supplied by non‐structural protein 12 (nsp12) that includes an RdRp domain conserved in all RNA viruses. Using SARS coronavirus, we now show that coronaviruses uniquely encode a second RdRp residing in nsp8. This protein strongly prefers the internal 5′‐(G/U)CC‐3′ trinucleotides on RNA templates to initiate the synthesis of complementary oligonucleotides of <6 residues in a reaction whose fidelity is relatively low. Distant structural homology between the C‐terminal domain of nsp8 and the catalytic palm subdomain of RdRps of RNA viruses suggests a common origin of the two coronavirus RdRps, which however may have evolved different sets of catalytic residues. A parallel between the nsp8 RdRp and cellular DNA‐dependent RNA primases is drawn to propose that the nsp8 RdRp produces primers utilized by the primer‐dependent nsp12 RdRp.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bourhis, Jean Arie" sort="Bourhis, Jean Arie" uniqKey="Bourhis J" first="Jean-Marie" last="Bourhis">Jean-Marie Bourhis</name><name sortKey="Bussetta, Cecile" sort="Bussetta, Cecile" uniqKey="Bussetta C" first="Cécile" last="Bussetta">Cécile Bussetta</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><name sortKey="Egloff, Marie Ierre" sort="Egloff, Marie Ierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E" last="Gorbalenya">Alexander E. Gorbalenya</name><name sortKey="Guillemot, Jean Laude" sort="Guillemot, Jean Laude" uniqKey="Guillemot J" first="Jean-Claude" last="Guillemot">Jean-Claude Guillemot</name><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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