Amotosalen photochemical inactivation of severe acute respiratory syndrome coronavirus in human platelet concentrates.
Identifieur interne : 001112 ( Ncbi/Checkpoint ); précédent : 001111; suivant : 001113Amotosalen photochemical inactivation of severe acute respiratory syndrome coronavirus in human platelet concentrates.
Auteurs : D. Pinna [Italie] ; A. Sampson-Johannes ; M. Clementi ; G. Poli ; S. Rossini ; L. Lin ; E. VicenziSource :
- Transfusion medicine (Oxford, England) [ 0958-7578 ] ; 2005.
Descripteurs français
- KwdFr :
- Conservation de sang (), Furocoumarines (pharmacologie), Humains, Inactivation virale (), Inactivation virale (effets des radiations), Photolyse (), Photosensibilisants (pharmacologie), Plaquettes (virologie), Stérilisation (), Thrombocytaphérèse (), Transfusion de plaquettes (), Virus du SRAS (), Virus du SRAS (effets des radiations).
- MESH :
- effets des radiations : Inactivation virale, Virus du SRAS.
- pharmacologie : Furocoumarines, Photosensibilisants.
- virologie : Plaquettes.
- Conservation de sang, Humains, Inactivation virale, Photolyse, Stérilisation, Thrombocytaphérèse, Transfusion de plaquettes, Virus du SRAS.
English descriptors
- KwdEn :
- Blood Platelets (virology), Blood Preservation (methods), Furocoumarins (pharmacology), Humans, Photolysis (drug effects), Photosensitizing Agents (pharmacology), Platelet Transfusion (methods), Plateletpheresis (methods), SARS Virus (drug effects), SARS Virus (radiation effects), Sterilization (methods), Virus Inactivation (drug effects), Virus Inactivation (radiation effects).
- MESH :
- chemical , pharmacology : Furocoumarins, Photosensitizing Agents.
- drug effects : Photolysis, SARS Virus, Virus Inactivation.
- methods : Blood Preservation, Platelet Transfusion, Plateletpheresis, Sterilization.
- radiation effects : SARS Virus, Virus Inactivation.
- virology : Blood Platelets.
- Humans.
Abstract
A novel human coronavirus causing severe acute respiratory syndrome (SARS) emerged in epidemic form in early 2003 in China and spread worldwide in a few months. Every newly emerging human pathogen is of concern for the safety of the blood supply during and after an epidemic crisis. For this purpose, we have evaluated the inactivation of SARS-coronavirus (CoV) in platelet concentrates using an approved pathogen inactivation device, the INTERCEPT Blood System. Apheresis platelet concentrates (APCs) were inoculated with approximately 10(6) pfu mL(-1) of either Urbani or HSR1 isolates of SARS-CoV. The inoculated units were mixed with 150 microm amotosalen and illuminated with 3 J cm(-2) UV-A light. The viral titres were determined by plaque formation in Vero E6 cells. Mixing SARS-CoV with APC in the absence of any treatment decreased viral infectivity by approximately 0.5-1 log10. Following photochemical treatment, SARS-CoV was consistently inactivated to the limit of detection in seven independent APC units. No infectious virus was detected after treatment when up to one-third of the APC unit was assayed, demonstrating a mean log10-reduction of >6.2. Potent inactivation of SARS-CoV therefore extends the capability of the INTERCEPT Blood System in inactivating a broad spectrum of human pathogens including recently emerging respiratory viruses.
DOI: 10.1111/j.0958-7578.2005.00588.x
PubMed: 16101804
Affiliations:
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pubmed:16101804Le document en format XML
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<front><div type="abstract" xml:lang="en">A novel human coronavirus causing severe acute respiratory syndrome (SARS) emerged in epidemic form in early 2003 in China and spread worldwide in a few months. Every newly emerging human pathogen is of concern for the safety of the blood supply during and after an epidemic crisis. For this purpose, we have evaluated the inactivation of SARS-coronavirus (CoV) in platelet concentrates using an approved pathogen inactivation device, the INTERCEPT Blood System. Apheresis platelet concentrates (APCs) were inoculated with approximately 10(6) pfu mL(-1) of either Urbani or HSR1 isolates of SARS-CoV. The inoculated units were mixed with 150 microm amotosalen and illuminated with 3 J cm(-2) UV-A light. The viral titres were determined by plaque formation in Vero E6 cells. Mixing SARS-CoV with APC in the absence of any treatment decreased viral infectivity by approximately 0.5-1 log10. Following photochemical treatment, SARS-CoV was consistently inactivated to the limit of detection in seven independent APC units. No infectious virus was detected after treatment when up to one-third of the APC unit was assayed, demonstrating a mean log10-reduction of >6.2. Potent inactivation of SARS-CoV therefore extends the capability of the INTERCEPT Blood System in inactivating a broad spectrum of human pathogens including recently emerging respiratory viruses.</div>
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